Cargando…

Agua Salud alphavirus defines a novel lineage of insect-specific alphaviruses discovered in the New World

The genus Alphavirus harbours mostly insect-transmitted viruses that cause severe disease in humans, livestock and wildlife. Thus far, only three alphaviruses with a host range restricted to insects have been found in mosquitoes from the Old World, namely Eilat virus (EILV), Taï Forest alphavirus (T...

Descripción completa

Detalles Bibliográficos
Autores principales: Hermanns, Kyra, Marklewitz, Marco, Zirkel, Florian, Overheul, Gijs J., Page, Rachel A., Loaiza, Jose R., Drosten, Christian, van Rij, Ronald P., Junglen, Sandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414432/
https://www.ncbi.nlm.nih.gov/pubmed/31674898
http://dx.doi.org/10.1099/jgv.0.001344
_version_ 1783568978183979008
author Hermanns, Kyra
Marklewitz, Marco
Zirkel, Florian
Overheul, Gijs J.
Page, Rachel A.
Loaiza, Jose R.
Drosten, Christian
van Rij, Ronald P.
Junglen, Sandra
author_facet Hermanns, Kyra
Marklewitz, Marco
Zirkel, Florian
Overheul, Gijs J.
Page, Rachel A.
Loaiza, Jose R.
Drosten, Christian
van Rij, Ronald P.
Junglen, Sandra
author_sort Hermanns, Kyra
collection PubMed
description The genus Alphavirus harbours mostly insect-transmitted viruses that cause severe disease in humans, livestock and wildlife. Thus far, only three alphaviruses with a host range restricted to insects have been found in mosquitoes from the Old World, namely Eilat virus (EILV), Taï Forest alphavirus (TALV) and Mwinilunga alphavirus (MWAV). In this study, we found a novel alphavirus in one Culex declarator mosquito sampled in Panama. The virus was isolated in C6/36 mosquito cells, and full genome sequencing revealed an 11 468 nt long genome with maximum pairwise nucleotide identity of 62.7 % to Sindbis virus. Phylogenetic analyses placed the virus as a solitary deep rooting lineage in a basal relationship to the Western equine encephalitis antigenic complex and to the clade comprising EILV, TALV and MWAV, indicating the detection of a novel alphavirus, tentatively named Agua Salud alphavirus (ASALV). No growth of ASALV was detected in vertebrate cell lines, including cell lines derived from ectothermic animals, and replication of ASALV was strongly impaired above 31 °C, suggesting that ASALV represents the first insect-restricted alphavirus of the New World.
format Online
Article
Text
id pubmed-7414432
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Microbiology Society
record_format MEDLINE/PubMed
spelling pubmed-74144322020-08-10 Agua Salud alphavirus defines a novel lineage of insect-specific alphaviruses discovered in the New World Hermanns, Kyra Marklewitz, Marco Zirkel, Florian Overheul, Gijs J. Page, Rachel A. Loaiza, Jose R. Drosten, Christian van Rij, Ronald P. Junglen, Sandra J Gen Virol Research Article The genus Alphavirus harbours mostly insect-transmitted viruses that cause severe disease in humans, livestock and wildlife. Thus far, only three alphaviruses with a host range restricted to insects have been found in mosquitoes from the Old World, namely Eilat virus (EILV), Taï Forest alphavirus (TALV) and Mwinilunga alphavirus (MWAV). In this study, we found a novel alphavirus in one Culex declarator mosquito sampled in Panama. The virus was isolated in C6/36 mosquito cells, and full genome sequencing revealed an 11 468 nt long genome with maximum pairwise nucleotide identity of 62.7 % to Sindbis virus. Phylogenetic analyses placed the virus as a solitary deep rooting lineage in a basal relationship to the Western equine encephalitis antigenic complex and to the clade comprising EILV, TALV and MWAV, indicating the detection of a novel alphavirus, tentatively named Agua Salud alphavirus (ASALV). No growth of ASALV was detected in vertebrate cell lines, including cell lines derived from ectothermic animals, and replication of ASALV was strongly impaired above 31 °C, suggesting that ASALV represents the first insect-restricted alphavirus of the New World. Microbiology Society 2020-01 2019-11-01 /pmc/articles/PMC7414432/ /pubmed/31674898 http://dx.doi.org/10.1099/jgv.0.001344 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
spellingShingle Research Article
Hermanns, Kyra
Marklewitz, Marco
Zirkel, Florian
Overheul, Gijs J.
Page, Rachel A.
Loaiza, Jose R.
Drosten, Christian
van Rij, Ronald P.
Junglen, Sandra
Agua Salud alphavirus defines a novel lineage of insect-specific alphaviruses discovered in the New World
title Agua Salud alphavirus defines a novel lineage of insect-specific alphaviruses discovered in the New World
title_full Agua Salud alphavirus defines a novel lineage of insect-specific alphaviruses discovered in the New World
title_fullStr Agua Salud alphavirus defines a novel lineage of insect-specific alphaviruses discovered in the New World
title_full_unstemmed Agua Salud alphavirus defines a novel lineage of insect-specific alphaviruses discovered in the New World
title_short Agua Salud alphavirus defines a novel lineage of insect-specific alphaviruses discovered in the New World
title_sort agua salud alphavirus defines a novel lineage of insect-specific alphaviruses discovered in the new world
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414432/
https://www.ncbi.nlm.nih.gov/pubmed/31674898
http://dx.doi.org/10.1099/jgv.0.001344
work_keys_str_mv AT hermannskyra aguasaludalphavirusdefinesanovellineageofinsectspecificalphavirusesdiscoveredinthenewworld
AT marklewitzmarco aguasaludalphavirusdefinesanovellineageofinsectspecificalphavirusesdiscoveredinthenewworld
AT zirkelflorian aguasaludalphavirusdefinesanovellineageofinsectspecificalphavirusesdiscoveredinthenewworld
AT overheulgijsj aguasaludalphavirusdefinesanovellineageofinsectspecificalphavirusesdiscoveredinthenewworld
AT pagerachela aguasaludalphavirusdefinesanovellineageofinsectspecificalphavirusesdiscoveredinthenewworld
AT loaizajoser aguasaludalphavirusdefinesanovellineageofinsectspecificalphavirusesdiscoveredinthenewworld
AT drostenchristian aguasaludalphavirusdefinesanovellineageofinsectspecificalphavirusesdiscoveredinthenewworld
AT vanrijronaldp aguasaludalphavirusdefinesanovellineageofinsectspecificalphavirusesdiscoveredinthenewworld
AT junglensandra aguasaludalphavirusdefinesanovellineageofinsectspecificalphavirusesdiscoveredinthenewworld