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Biospeckle optical coherence tomography in speedy visualizing effects of foliar application of plant growth hormone to Chinese chives leaves

OBJECTIVE: The aim of this study is to demonstrate the potential of applying the contrast of the speckles obtained as noise in optical coherence tomography (OCT) images to monitor short term activity changes during foliar application of phytohormones to a plant leaf. Plant growth hormone, gibberelli...

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Autores principales: Rajagopalan, Uma Maheswari, Kabir, Mahjabin, Lim, Yiheng, Kadono, Hirofumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414531/
https://www.ncbi.nlm.nih.gov/pubmed/32771056
http://dx.doi.org/10.1186/s13104-020-05219-7
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author Rajagopalan, Uma Maheswari
Kabir, Mahjabin
Lim, Yiheng
Kadono, Hirofumi
author_facet Rajagopalan, Uma Maheswari
Kabir, Mahjabin
Lim, Yiheng
Kadono, Hirofumi
author_sort Rajagopalan, Uma Maheswari
collection PubMed
description OBJECTIVE: The aim of this study is to demonstrate the potential of applying the contrast of the speckles obtained as noise in optical coherence tomography (OCT) images to monitor short term activity changes during foliar application of phytohormones to a plant leaf. Plant growth hormone, gibberellic acid (GA(3)) was sprayed onto the leaf of Chinese chives and after 60 min, OCT images (1 frame: 512 × 2048 pixels) were recorded at ten frames per second for a few tens of seconds. RESULTS: Contrast across the temporal axis was calculated for each pixel of the structural images and biospeckle OCT contrast images were obtained under the conditions of before and after application of GA(3) for different concentrations 0, 40, and 100 μM. Application of 40 μM GA(3) failed to show any differences in the OCT structural images. However, bOCT contrast image was clearly different. Changes were found to be statistically significant. Although the mechanism for the contrast difference is not clear, it can be said there is a large change across the temporal scale with the application of GA(3). Demonstration of OCT utilizing the speckle contrast is believed to have the potential as a promising tool in plant physiology.
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spelling pubmed-74145312020-08-10 Biospeckle optical coherence tomography in speedy visualizing effects of foliar application of plant growth hormone to Chinese chives leaves Rajagopalan, Uma Maheswari Kabir, Mahjabin Lim, Yiheng Kadono, Hirofumi BMC Res Notes Research Note OBJECTIVE: The aim of this study is to demonstrate the potential of applying the contrast of the speckles obtained as noise in optical coherence tomography (OCT) images to monitor short term activity changes during foliar application of phytohormones to a plant leaf. Plant growth hormone, gibberellic acid (GA(3)) was sprayed onto the leaf of Chinese chives and after 60 min, OCT images (1 frame: 512 × 2048 pixels) were recorded at ten frames per second for a few tens of seconds. RESULTS: Contrast across the temporal axis was calculated for each pixel of the structural images and biospeckle OCT contrast images were obtained under the conditions of before and after application of GA(3) for different concentrations 0, 40, and 100 μM. Application of 40 μM GA(3) failed to show any differences in the OCT structural images. However, bOCT contrast image was clearly different. Changes were found to be statistically significant. Although the mechanism for the contrast difference is not clear, it can be said there is a large change across the temporal scale with the application of GA(3). Demonstration of OCT utilizing the speckle contrast is believed to have the potential as a promising tool in plant physiology. BioMed Central 2020-08-08 /pmc/articles/PMC7414531/ /pubmed/32771056 http://dx.doi.org/10.1186/s13104-020-05219-7 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Rajagopalan, Uma Maheswari
Kabir, Mahjabin
Lim, Yiheng
Kadono, Hirofumi
Biospeckle optical coherence tomography in speedy visualizing effects of foliar application of plant growth hormone to Chinese chives leaves
title Biospeckle optical coherence tomography in speedy visualizing effects of foliar application of plant growth hormone to Chinese chives leaves
title_full Biospeckle optical coherence tomography in speedy visualizing effects of foliar application of plant growth hormone to Chinese chives leaves
title_fullStr Biospeckle optical coherence tomography in speedy visualizing effects of foliar application of plant growth hormone to Chinese chives leaves
title_full_unstemmed Biospeckle optical coherence tomography in speedy visualizing effects of foliar application of plant growth hormone to Chinese chives leaves
title_short Biospeckle optical coherence tomography in speedy visualizing effects of foliar application of plant growth hormone to Chinese chives leaves
title_sort biospeckle optical coherence tomography in speedy visualizing effects of foliar application of plant growth hormone to chinese chives leaves
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414531/
https://www.ncbi.nlm.nih.gov/pubmed/32771056
http://dx.doi.org/10.1186/s13104-020-05219-7
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