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Effects of HPV16 E6 protein on Daxx-induced apoptosis in C33A cells

AIMS: Daxx is a highly conserved nuclear protein with an important role in transcription, apoptosis and other cell processes. We investigated the role of HPV16 E6 in Daxx-induced apoptosis through their interactions in C33A cells. METHODS: The binding of HPV16 E6 and Daxx was confirmed in C33A cells...

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Autores principales: Tang, Shuangyang, Ding, Shuang, Yu, Lan, Shen, Haiyan, Wan, Yanping, Wu, Yimou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414724/
https://www.ncbi.nlm.nih.gov/pubmed/32782452
http://dx.doi.org/10.1186/s11658-020-00230-z
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author Tang, Shuangyang
Ding, Shuang
Yu, Lan
Shen, Haiyan
Wan, Yanping
Wu, Yimou
author_facet Tang, Shuangyang
Ding, Shuang
Yu, Lan
Shen, Haiyan
Wan, Yanping
Wu, Yimou
author_sort Tang, Shuangyang
collection PubMed
description AIMS: Daxx is a highly conserved nuclear protein with an important role in transcription, apoptosis and other cell processes. We investigated the role of HPV16 E6 in Daxx-induced apoptosis through their interactions in C33A cells. METHODS: The binding of HPV16 E6 and Daxx was confirmed in C33A cells using co-immunoprecipitation and indirect immunofluorescence assays. Quantitative PCR and western blotting were performed to determine the RNA and protein expressions of Daxx, respectively. Automatic cell count and MTT assays were performed to investigate the proliferation of C33A cells. The apoptosis rate of C33A cells was determined via flow cytometry using Annexin V-FITC/PI staining. The relative activity of caspase-8 was tested using ELISA. RESULTS: HPV16 E6 can bind with Daxx and cause its translocation in C33A cells. The transfected HPV16 E6 can cause a decrease in relative quantification for Daxx in Daxx-overexpressing cells. After Daxx transfection, cell proliferation was found to decrease sharply and cell apoptosis to increase sharply. However, when HPV16 E6 was co-transfected with Daxx, this decrease and increase both became gentle. Similarly, HPV16 E6 made the Daxx-induced increase in caspase-8 activity milder. CONCLUSIONS: HPV16 E6 is involved in inhibiting apoptosis through deregulation of Daxx-induced caspase-8 activities.
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spelling pubmed-74147242020-08-10 Effects of HPV16 E6 protein on Daxx-induced apoptosis in C33A cells Tang, Shuangyang Ding, Shuang Yu, Lan Shen, Haiyan Wan, Yanping Wu, Yimou Cell Mol Biol Lett Research Letter AIMS: Daxx is a highly conserved nuclear protein with an important role in transcription, apoptosis and other cell processes. We investigated the role of HPV16 E6 in Daxx-induced apoptosis through their interactions in C33A cells. METHODS: The binding of HPV16 E6 and Daxx was confirmed in C33A cells using co-immunoprecipitation and indirect immunofluorescence assays. Quantitative PCR and western blotting were performed to determine the RNA and protein expressions of Daxx, respectively. Automatic cell count and MTT assays were performed to investigate the proliferation of C33A cells. The apoptosis rate of C33A cells was determined via flow cytometry using Annexin V-FITC/PI staining. The relative activity of caspase-8 was tested using ELISA. RESULTS: HPV16 E6 can bind with Daxx and cause its translocation in C33A cells. The transfected HPV16 E6 can cause a decrease in relative quantification for Daxx in Daxx-overexpressing cells. After Daxx transfection, cell proliferation was found to decrease sharply and cell apoptosis to increase sharply. However, when HPV16 E6 was co-transfected with Daxx, this decrease and increase both became gentle. Similarly, HPV16 E6 made the Daxx-induced increase in caspase-8 activity milder. CONCLUSIONS: HPV16 E6 is involved in inhibiting apoptosis through deregulation of Daxx-induced caspase-8 activities. BioMed Central 2020-08-08 /pmc/articles/PMC7414724/ /pubmed/32782452 http://dx.doi.org/10.1186/s11658-020-00230-z Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Letter
Tang, Shuangyang
Ding, Shuang
Yu, Lan
Shen, Haiyan
Wan, Yanping
Wu, Yimou
Effects of HPV16 E6 protein on Daxx-induced apoptosis in C33A cells
title Effects of HPV16 E6 protein on Daxx-induced apoptosis in C33A cells
title_full Effects of HPV16 E6 protein on Daxx-induced apoptosis in C33A cells
title_fullStr Effects of HPV16 E6 protein on Daxx-induced apoptosis in C33A cells
title_full_unstemmed Effects of HPV16 E6 protein on Daxx-induced apoptosis in C33A cells
title_short Effects of HPV16 E6 protein on Daxx-induced apoptosis in C33A cells
title_sort effects of hpv16 e6 protein on daxx-induced apoptosis in c33a cells
topic Research Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414724/
https://www.ncbi.nlm.nih.gov/pubmed/32782452
http://dx.doi.org/10.1186/s11658-020-00230-z
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