Cargando…

Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties

BACKGROUND: Diesel exhaust is carcinogenic and exposure to diesel particles cause health effects. We investigated the toxicity of diesel exhaust particles designed to have varying physicochemical properties in order to attribute health effects to specific particle characteristics. Particles from thr...

Descripción completa

Detalles Bibliográficos
Autores principales: Bendtsen, Katja Maria, Gren, Louise, Malmborg, Vilhelm Berg, Shukla, Pravesh Chandra, Tunér, Martin, Essig, Yona J., Krais, Annette M., Clausen, Per Axel, Berthing, Trine, Loeschner, Katrin, Jacobsen, Nicklas Raun, Wolff, Henrik, Pagels, Joakim, Vogel, Ulla Birgitte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414762/
https://www.ncbi.nlm.nih.gov/pubmed/32771016
http://dx.doi.org/10.1186/s12989-020-00369-9
_version_ 1783569036107317248
author Bendtsen, Katja Maria
Gren, Louise
Malmborg, Vilhelm Berg
Shukla, Pravesh Chandra
Tunér, Martin
Essig, Yona J.
Krais, Annette M.
Clausen, Per Axel
Berthing, Trine
Loeschner, Katrin
Jacobsen, Nicklas Raun
Wolff, Henrik
Pagels, Joakim
Vogel, Ulla Birgitte
author_facet Bendtsen, Katja Maria
Gren, Louise
Malmborg, Vilhelm Berg
Shukla, Pravesh Chandra
Tunér, Martin
Essig, Yona J.
Krais, Annette M.
Clausen, Per Axel
Berthing, Trine
Loeschner, Katrin
Jacobsen, Nicklas Raun
Wolff, Henrik
Pagels, Joakim
Vogel, Ulla Birgitte
author_sort Bendtsen, Katja Maria
collection PubMed
description BACKGROUND: Diesel exhaust is carcinogenic and exposure to diesel particles cause health effects. We investigated the toxicity of diesel exhaust particles designed to have varying physicochemical properties in order to attribute health effects to specific particle characteristics. Particles from three fuel types were compared at 13% engine intake O(2) concentration: MK1 ultra low sulfur diesel (DEP13) and the two renewable diesel fuels hydrotreated vegetable oil (HVO13) and rapeseed methyl ester (RME13). Additionally, diesel particles from MK1 ultra low sulfur diesel were generated at 9.7% (DEP9.7) and 17% (DEP17) intake O(2) concentration. We evaluated physicochemical properties and histopathological, inflammatory and genotoxic responses on day 1, 28, and 90 after single intratracheal instillation in mice compared to reference diesel particles and carbon black. RESULTS: Moderate variations were seen in physical properties for the five particles: primary particle diameter: 15–22 nm, specific surface area: 152–222 m(2)/g, and count median mobility diameter: 55–103 nm. Larger differences were found in chemical composition: organic carbon/total carbon ratio (0.12–0.60), polycyclic aromatic hydrocarbon content (1–27 μg/mg) and acid-extractable metal content (0.9–16 μg/mg). Intratracheal exposure to all five particles induced similar toxicological responses, with different potency. Lung particle retention was observed in DEP13 and HVO13 exposed mice on day 28 post-exposure, with less retention for the other fuel types. RME exposure induced limited response whereas the remaining particles induced dose-dependent inflammation and acute phase response on day 1. DEP13 induced acute phase response on day 28 and inflammation on day 90. DNA strand break levels were not increased as compared to vehicle, but were increased in lung and liver compared to blank filter extraction control. Neutrophil influx on day 1 correlated best with estimated deposited surface area, but also with elemental carbon, organic carbon and PAHs. DNA strand break levels in lung on day 28 and in liver on day 90 correlated with acellular particle-induced ROS. CONCLUSIONS: We studied diesel exhaust particles designed to differ in physicochemical properties. Our study highlights specific surface area, elemental carbon content, PAHs and ROS-generating potential as physicochemical predictors of diesel particle toxicity.
format Online
Article
Text
id pubmed-7414762
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-74147622020-08-10 Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties Bendtsen, Katja Maria Gren, Louise Malmborg, Vilhelm Berg Shukla, Pravesh Chandra Tunér, Martin Essig, Yona J. Krais, Annette M. Clausen, Per Axel Berthing, Trine Loeschner, Katrin Jacobsen, Nicklas Raun Wolff, Henrik Pagels, Joakim Vogel, Ulla Birgitte Part Fibre Toxicol Research BACKGROUND: Diesel exhaust is carcinogenic and exposure to diesel particles cause health effects. We investigated the toxicity of diesel exhaust particles designed to have varying physicochemical properties in order to attribute health effects to specific particle characteristics. Particles from three fuel types were compared at 13% engine intake O(2) concentration: MK1 ultra low sulfur diesel (DEP13) and the two renewable diesel fuels hydrotreated vegetable oil (HVO13) and rapeseed methyl ester (RME13). Additionally, diesel particles from MK1 ultra low sulfur diesel were generated at 9.7% (DEP9.7) and 17% (DEP17) intake O(2) concentration. We evaluated physicochemical properties and histopathological, inflammatory and genotoxic responses on day 1, 28, and 90 after single intratracheal instillation in mice compared to reference diesel particles and carbon black. RESULTS: Moderate variations were seen in physical properties for the five particles: primary particle diameter: 15–22 nm, specific surface area: 152–222 m(2)/g, and count median mobility diameter: 55–103 nm. Larger differences were found in chemical composition: organic carbon/total carbon ratio (0.12–0.60), polycyclic aromatic hydrocarbon content (1–27 μg/mg) and acid-extractable metal content (0.9–16 μg/mg). Intratracheal exposure to all five particles induced similar toxicological responses, with different potency. Lung particle retention was observed in DEP13 and HVO13 exposed mice on day 28 post-exposure, with less retention for the other fuel types. RME exposure induced limited response whereas the remaining particles induced dose-dependent inflammation and acute phase response on day 1. DEP13 induced acute phase response on day 28 and inflammation on day 90. DNA strand break levels were not increased as compared to vehicle, but were increased in lung and liver compared to blank filter extraction control. Neutrophil influx on day 1 correlated best with estimated deposited surface area, but also with elemental carbon, organic carbon and PAHs. DNA strand break levels in lung on day 28 and in liver on day 90 correlated with acellular particle-induced ROS. CONCLUSIONS: We studied diesel exhaust particles designed to differ in physicochemical properties. Our study highlights specific surface area, elemental carbon content, PAHs and ROS-generating potential as physicochemical predictors of diesel particle toxicity. BioMed Central 2020-08-08 /pmc/articles/PMC7414762/ /pubmed/32771016 http://dx.doi.org/10.1186/s12989-020-00369-9 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Bendtsen, Katja Maria
Gren, Louise
Malmborg, Vilhelm Berg
Shukla, Pravesh Chandra
Tunér, Martin
Essig, Yona J.
Krais, Annette M.
Clausen, Per Axel
Berthing, Trine
Loeschner, Katrin
Jacobsen, Nicklas Raun
Wolff, Henrik
Pagels, Joakim
Vogel, Ulla Birgitte
Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties
title Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties
title_full Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties
title_fullStr Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties
title_full_unstemmed Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties
title_short Particle characterization and toxicity in C57BL/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties
title_sort particle characterization and toxicity in c57bl/6 mice following instillation of five different diesel exhaust particles designed to differ in physicochemical properties
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414762/
https://www.ncbi.nlm.nih.gov/pubmed/32771016
http://dx.doi.org/10.1186/s12989-020-00369-9
work_keys_str_mv AT bendtsenkatjamaria particlecharacterizationandtoxicityinc57bl6micefollowinginstillationoffivedifferentdieselexhaustparticlesdesignedtodifferinphysicochemicalproperties
AT grenlouise particlecharacterizationandtoxicityinc57bl6micefollowinginstillationoffivedifferentdieselexhaustparticlesdesignedtodifferinphysicochemicalproperties
AT malmborgvilhelmberg particlecharacterizationandtoxicityinc57bl6micefollowinginstillationoffivedifferentdieselexhaustparticlesdesignedtodifferinphysicochemicalproperties
AT shuklapraveshchandra particlecharacterizationandtoxicityinc57bl6micefollowinginstillationoffivedifferentdieselexhaustparticlesdesignedtodifferinphysicochemicalproperties
AT tunermartin particlecharacterizationandtoxicityinc57bl6micefollowinginstillationoffivedifferentdieselexhaustparticlesdesignedtodifferinphysicochemicalproperties
AT essigyonaj particlecharacterizationandtoxicityinc57bl6micefollowinginstillationoffivedifferentdieselexhaustparticlesdesignedtodifferinphysicochemicalproperties
AT kraisannettem particlecharacterizationandtoxicityinc57bl6micefollowinginstillationoffivedifferentdieselexhaustparticlesdesignedtodifferinphysicochemicalproperties
AT clausenperaxel particlecharacterizationandtoxicityinc57bl6micefollowinginstillationoffivedifferentdieselexhaustparticlesdesignedtodifferinphysicochemicalproperties
AT berthingtrine particlecharacterizationandtoxicityinc57bl6micefollowinginstillationoffivedifferentdieselexhaustparticlesdesignedtodifferinphysicochemicalproperties
AT loeschnerkatrin particlecharacterizationandtoxicityinc57bl6micefollowinginstillationoffivedifferentdieselexhaustparticlesdesignedtodifferinphysicochemicalproperties
AT jacobsennicklasraun particlecharacterizationandtoxicityinc57bl6micefollowinginstillationoffivedifferentdieselexhaustparticlesdesignedtodifferinphysicochemicalproperties
AT wolffhenrik particlecharacterizationandtoxicityinc57bl6micefollowinginstillationoffivedifferentdieselexhaustparticlesdesignedtodifferinphysicochemicalproperties
AT pagelsjoakim particlecharacterizationandtoxicityinc57bl6micefollowinginstillationoffivedifferentdieselexhaustparticlesdesignedtodifferinphysicochemicalproperties
AT vogelullabirgitte particlecharacterizationandtoxicityinc57bl6micefollowinginstillationoffivedifferentdieselexhaustparticlesdesignedtodifferinphysicochemicalproperties