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Spray dried VSV-vectored vaccine is thermally stable and immunologically active in vivo

Effective vaccine delivery and coverage to rural and resource-poor countries is hindered by the dependence on cold chain storage. As such, developments of cold chain-free technologies are highly sought. Although spray dried adenoviral vectors have shown long term stability at ambient temperatures an...

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Autores principales: Toniolo, Steven P., Afkhami, Sam, D’Agostino, Michael R., Lichty, Brian D., Cranston, Emily D., Xing, Zhou, Thompson, Michael R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414861/
https://www.ncbi.nlm.nih.gov/pubmed/32770018
http://dx.doi.org/10.1038/s41598-020-70325-2
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author Toniolo, Steven P.
Afkhami, Sam
D’Agostino, Michael R.
Lichty, Brian D.
Cranston, Emily D.
Xing, Zhou
Thompson, Michael R.
author_facet Toniolo, Steven P.
Afkhami, Sam
D’Agostino, Michael R.
Lichty, Brian D.
Cranston, Emily D.
Xing, Zhou
Thompson, Michael R.
author_sort Toniolo, Steven P.
collection PubMed
description Effective vaccine delivery and coverage to rural and resource-poor countries is hindered by the dependence on cold chain storage. As such, developments of cold chain-free technologies are highly sought. Although spray dried adenoviral vectors have shown long term stability at ambient temperatures and relatively low humidity, it remains to be determined whether similar excipient formulations are applicable to other viral vectors. To address this, we have spray dried vesicular stomatitis virus (VSV)-vectors with a panel of well-characterized sugar excipients to determine the optimal formulation for vector stabilization. Upon reconstitution, we show that trehalose conferred superior stability of VSV both in vitro and in vivo. Importantly, following cold chain-free storage at elevated temperatures at 37 °C for 15 days, we show that a VSV-vectored vaccine retains its in vivo immunogenicity, whereas a liquid control completely lost its immune-stimulating ability. Our results provide foundational evidence that spray drying with properly tested excipients can stabilize viral vectors such as VSV, allowing them to be stored long-term at elevated temperatures without dependency on cold chain conditions.
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spelling pubmed-74148612020-08-11 Spray dried VSV-vectored vaccine is thermally stable and immunologically active in vivo Toniolo, Steven P. Afkhami, Sam D’Agostino, Michael R. Lichty, Brian D. Cranston, Emily D. Xing, Zhou Thompson, Michael R. Sci Rep Article Effective vaccine delivery and coverage to rural and resource-poor countries is hindered by the dependence on cold chain storage. As such, developments of cold chain-free technologies are highly sought. Although spray dried adenoviral vectors have shown long term stability at ambient temperatures and relatively low humidity, it remains to be determined whether similar excipient formulations are applicable to other viral vectors. To address this, we have spray dried vesicular stomatitis virus (VSV)-vectors with a panel of well-characterized sugar excipients to determine the optimal formulation for vector stabilization. Upon reconstitution, we show that trehalose conferred superior stability of VSV both in vitro and in vivo. Importantly, following cold chain-free storage at elevated temperatures at 37 °C for 15 days, we show that a VSV-vectored vaccine retains its in vivo immunogenicity, whereas a liquid control completely lost its immune-stimulating ability. Our results provide foundational evidence that spray drying with properly tested excipients can stabilize viral vectors such as VSV, allowing them to be stored long-term at elevated temperatures without dependency on cold chain conditions. Nature Publishing Group UK 2020-08-07 /pmc/articles/PMC7414861/ /pubmed/32770018 http://dx.doi.org/10.1038/s41598-020-70325-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Toniolo, Steven P.
Afkhami, Sam
D’Agostino, Michael R.
Lichty, Brian D.
Cranston, Emily D.
Xing, Zhou
Thompson, Michael R.
Spray dried VSV-vectored vaccine is thermally stable and immunologically active in vivo
title Spray dried VSV-vectored vaccine is thermally stable and immunologically active in vivo
title_full Spray dried VSV-vectored vaccine is thermally stable and immunologically active in vivo
title_fullStr Spray dried VSV-vectored vaccine is thermally stable and immunologically active in vivo
title_full_unstemmed Spray dried VSV-vectored vaccine is thermally stable and immunologically active in vivo
title_short Spray dried VSV-vectored vaccine is thermally stable and immunologically active in vivo
title_sort spray dried vsv-vectored vaccine is thermally stable and immunologically active in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414861/
https://www.ncbi.nlm.nih.gov/pubmed/32770018
http://dx.doi.org/10.1038/s41598-020-70325-2
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