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The Rnf complex is a Na(+) coupled respiratory enzyme in a fermenting bacterium, Thermotoga maritima

rnf genes are widespread in bacteria and biochemical and genetic data are in line with the hypothesis that they encode a membrane-bound enzyme that oxidizes reduced ferredoxin and reduces NAD and vice versa, coupled to ion transport across the cytoplasmic membrane. The Rnf complex is of critical imp...

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Autores principales: Kuhns, Martin, Trifunović, Dragan, Huber, Harald, Müller, Volker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414866/
https://www.ncbi.nlm.nih.gov/pubmed/32770029
http://dx.doi.org/10.1038/s42003-020-01158-y
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author Kuhns, Martin
Trifunović, Dragan
Huber, Harald
Müller, Volker
author_facet Kuhns, Martin
Trifunović, Dragan
Huber, Harald
Müller, Volker
author_sort Kuhns, Martin
collection PubMed
description rnf genes are widespread in bacteria and biochemical and genetic data are in line with the hypothesis that they encode a membrane-bound enzyme that oxidizes reduced ferredoxin and reduces NAD and vice versa, coupled to ion transport across the cytoplasmic membrane. The Rnf complex is of critical importance in many bacteria for energy conservation but also for reverse electron transport to drive ferredoxin reduction. However, the enzyme has never been purified and thus, ion transport could not be demonstrated yet. Here, we have purified the Rnf complex from the anaerobic, fermenting thermophilic bacterium Thermotoga maritima and show that is a primary Na(+) pump. These studies provide the proof that the Rnf complex is indeed an ion (Na(+)) translocating, respiratory enzyme. Together with a Na(+)-F(1)F(O) ATP synthase it builds a simple, two-limb respiratory chain in T. maritima. The physiological role of electron transport phosphorylation in a fermenting bacterium is discussed.
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spelling pubmed-74148662020-08-17 The Rnf complex is a Na(+) coupled respiratory enzyme in a fermenting bacterium, Thermotoga maritima Kuhns, Martin Trifunović, Dragan Huber, Harald Müller, Volker Commun Biol Article rnf genes are widespread in bacteria and biochemical and genetic data are in line with the hypothesis that they encode a membrane-bound enzyme that oxidizes reduced ferredoxin and reduces NAD and vice versa, coupled to ion transport across the cytoplasmic membrane. The Rnf complex is of critical importance in many bacteria for energy conservation but also for reverse electron transport to drive ferredoxin reduction. However, the enzyme has never been purified and thus, ion transport could not be demonstrated yet. Here, we have purified the Rnf complex from the anaerobic, fermenting thermophilic bacterium Thermotoga maritima and show that is a primary Na(+) pump. These studies provide the proof that the Rnf complex is indeed an ion (Na(+)) translocating, respiratory enzyme. Together with a Na(+)-F(1)F(O) ATP synthase it builds a simple, two-limb respiratory chain in T. maritima. The physiological role of electron transport phosphorylation in a fermenting bacterium is discussed. Nature Publishing Group UK 2020-08-07 /pmc/articles/PMC7414866/ /pubmed/32770029 http://dx.doi.org/10.1038/s42003-020-01158-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kuhns, Martin
Trifunović, Dragan
Huber, Harald
Müller, Volker
The Rnf complex is a Na(+) coupled respiratory enzyme in a fermenting bacterium, Thermotoga maritima
title The Rnf complex is a Na(+) coupled respiratory enzyme in a fermenting bacterium, Thermotoga maritima
title_full The Rnf complex is a Na(+) coupled respiratory enzyme in a fermenting bacterium, Thermotoga maritima
title_fullStr The Rnf complex is a Na(+) coupled respiratory enzyme in a fermenting bacterium, Thermotoga maritima
title_full_unstemmed The Rnf complex is a Na(+) coupled respiratory enzyme in a fermenting bacterium, Thermotoga maritima
title_short The Rnf complex is a Na(+) coupled respiratory enzyme in a fermenting bacterium, Thermotoga maritima
title_sort rnf complex is a na(+) coupled respiratory enzyme in a fermenting bacterium, thermotoga maritima
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414866/
https://www.ncbi.nlm.nih.gov/pubmed/32770029
http://dx.doi.org/10.1038/s42003-020-01158-y
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