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NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells
Jagged1 activates Notch signaling and subsequently promotes osteogenic differentiation in human periodontal ligament cells (hPDLs). The present study investigated the participation of the Notch receptor, NOTCH2, in the Jagged1-induced osteogenic differentiation in hPDLs. NOTCH2 and NOTCH4 mRNA expre...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414879/ https://www.ncbi.nlm.nih.gov/pubmed/32770090 http://dx.doi.org/10.1038/s41598-020-70277-7 |
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author | Manokawinchoke, Jeeranan Sumrejkanchanakij, Piyamas Boonprakong, Lawan Pavasant, Prasit Egusa, Hiroshi Osathanon, Thanaphum |
author_facet | Manokawinchoke, Jeeranan Sumrejkanchanakij, Piyamas Boonprakong, Lawan Pavasant, Prasit Egusa, Hiroshi Osathanon, Thanaphum |
author_sort | Manokawinchoke, Jeeranan |
collection | PubMed |
description | Jagged1 activates Notch signaling and subsequently promotes osteogenic differentiation in human periodontal ligament cells (hPDLs). The present study investigated the participation of the Notch receptor, NOTCH2, in the Jagged1-induced osteogenic differentiation in hPDLs. NOTCH2 and NOTCH4 mRNA expression levels increased during hPDL osteogenic differentiation. However, the endogenous NOTCH2 expression levels were markedly higher compared with NOTCH4. NOTCH2 expression knockdown using shRNA in hPDLs did not dramatically alter their proliferation or osteogenic differentiation compared with the shRNA control. After seeding on Jagged1-immobilized surfaces and maintaining the hPDLs in osteogenic medium, HES1 and HEY1 mRNA levels were markedly reduced in the shNOTCH2-transduced cells compared with the shControl group. Further, shNOTCH2-transduced cells exhibited less alkaline phosphatase enzymatic activity and in vitro mineralization than the shControl cells when exposed to Jagged1. MSX2 and COL1A1 mRNA expression after Jagged1 activation were reduced in shNOTCH2-transduced cells. Endogenous Notch signaling inhibition using a γ-secretase inhibitor (DAPT) attenuated mineralization in hPDLs. DAPT treatment significantly promoted TWIST1, but decreased ALP, mRNA expression, compared with the control. In conclusion, Notch signaling is involved in hPDL osteogenic differentiation. Moreover, NOTCH2 participates in the mechanism by which Jagged1 induced osteogenic differentiation in hPDLs. |
format | Online Article Text |
id | pubmed-7414879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-74148792020-08-11 NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells Manokawinchoke, Jeeranan Sumrejkanchanakij, Piyamas Boonprakong, Lawan Pavasant, Prasit Egusa, Hiroshi Osathanon, Thanaphum Sci Rep Article Jagged1 activates Notch signaling and subsequently promotes osteogenic differentiation in human periodontal ligament cells (hPDLs). The present study investigated the participation of the Notch receptor, NOTCH2, in the Jagged1-induced osteogenic differentiation in hPDLs. NOTCH2 and NOTCH4 mRNA expression levels increased during hPDL osteogenic differentiation. However, the endogenous NOTCH2 expression levels were markedly higher compared with NOTCH4. NOTCH2 expression knockdown using shRNA in hPDLs did not dramatically alter their proliferation or osteogenic differentiation compared with the shRNA control. After seeding on Jagged1-immobilized surfaces and maintaining the hPDLs in osteogenic medium, HES1 and HEY1 mRNA levels were markedly reduced in the shNOTCH2-transduced cells compared with the shControl group. Further, shNOTCH2-transduced cells exhibited less alkaline phosphatase enzymatic activity and in vitro mineralization than the shControl cells when exposed to Jagged1. MSX2 and COL1A1 mRNA expression after Jagged1 activation were reduced in shNOTCH2-transduced cells. Endogenous Notch signaling inhibition using a γ-secretase inhibitor (DAPT) attenuated mineralization in hPDLs. DAPT treatment significantly promoted TWIST1, but decreased ALP, mRNA expression, compared with the control. In conclusion, Notch signaling is involved in hPDL osteogenic differentiation. Moreover, NOTCH2 participates in the mechanism by which Jagged1 induced osteogenic differentiation in hPDLs. Nature Publishing Group UK 2020-08-07 /pmc/articles/PMC7414879/ /pubmed/32770090 http://dx.doi.org/10.1038/s41598-020-70277-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Manokawinchoke, Jeeranan Sumrejkanchanakij, Piyamas Boonprakong, Lawan Pavasant, Prasit Egusa, Hiroshi Osathanon, Thanaphum NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells |
title | NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells |
title_full | NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells |
title_fullStr | NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells |
title_full_unstemmed | NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells |
title_short | NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells |
title_sort | notch2 participates in jagged1-induced osteogenic differentiation in human periodontal ligament cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414879/ https://www.ncbi.nlm.nih.gov/pubmed/32770090 http://dx.doi.org/10.1038/s41598-020-70277-7 |
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