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Kaempferol separated from Camellia oleifera meal by high-speed countercurrent chromatography for antibacterial application

Natural biologically active substances have received continuous attention for the potentially beneficial health properties against chronic diseases. In this study, bacteriostatic active substance from Camellia oleifera meal, which is a major by-product of the Camellia oil processing industry, were e...

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Autores principales: Qiu, Yuanxin, He, Di, Yang, Jingxian, Ma, Lukai, Zhu, Kaiqi, Cao, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7415335/
https://www.ncbi.nlm.nih.gov/pubmed/32837313
http://dx.doi.org/10.1007/s00217-020-03582-0
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author Qiu, Yuanxin
He, Di
Yang, Jingxian
Ma, Lukai
Zhu, Kaiqi
Cao, Yong
author_facet Qiu, Yuanxin
He, Di
Yang, Jingxian
Ma, Lukai
Zhu, Kaiqi
Cao, Yong
author_sort Qiu, Yuanxin
collection PubMed
description Natural biologically active substances have received continuous attention for the potentially beneficial health properties against chronic diseases. In this study, bacteriostatic active substance from Camellia oleifera meal, which is a major by-product of the Camellia oil processing industry, were extracted with continuous phase change extraction (CPCE) method and separated by HSCCC. Compared with traditional extraction methods, CPCE possessed higher extraction efficiency. Two main substances were separated and purified (above 90.0%). The structure of them were further identified by UV, LC–ESI–MS–MS, 1H-NMR, and 13C-NMR as flavonoids F2 kaempferol 3-O-[β-d-glucopyranosyl-(1 → 2)-α-l-rhamnopyranosyl-(1 → 6)]-β-d-glucopyranoside and J2 kaempferol 3-O-[β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranosyl-(1 → 6)]-β-d-glucopyranoside for the first time in C. Oleifera meal. The results of antibacterial activity measurement showed that both compounds have excellent antibacterial activity. And the antibacterial stability of F2 were finally confirmed: F2 showed broad spectrum antibacterial activity against Escherichia coli, Staphylococcus aureus, Salmonella enteriditis, Bacillus thuringiensis, Aspergillus niger and Rhizopus nigricans. Besides, F2 exhibited relatively high stable property even at high temperature, acid and metal ion solutions. The findings of this work suggest the possibility of employing C. oleifera meal as an attractive source of health-promoting compounds, and at the same time facilitate its high-value reuse and reduction of environmental burden.
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spelling pubmed-74153352020-08-10 Kaempferol separated from Camellia oleifera meal by high-speed countercurrent chromatography for antibacterial application Qiu, Yuanxin He, Di Yang, Jingxian Ma, Lukai Zhu, Kaiqi Cao, Yong Eur Food Res Technol Original Paper Natural biologically active substances have received continuous attention for the potentially beneficial health properties against chronic diseases. In this study, bacteriostatic active substance from Camellia oleifera meal, which is a major by-product of the Camellia oil processing industry, were extracted with continuous phase change extraction (CPCE) method and separated by HSCCC. Compared with traditional extraction methods, CPCE possessed higher extraction efficiency. Two main substances were separated and purified (above 90.0%). The structure of them were further identified by UV, LC–ESI–MS–MS, 1H-NMR, and 13C-NMR as flavonoids F2 kaempferol 3-O-[β-d-glucopyranosyl-(1 → 2)-α-l-rhamnopyranosyl-(1 → 6)]-β-d-glucopyranoside and J2 kaempferol 3-O-[β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranosyl-(1 → 6)]-β-d-glucopyranoside for the first time in C. Oleifera meal. The results of antibacterial activity measurement showed that both compounds have excellent antibacterial activity. And the antibacterial stability of F2 were finally confirmed: F2 showed broad spectrum antibacterial activity against Escherichia coli, Staphylococcus aureus, Salmonella enteriditis, Bacillus thuringiensis, Aspergillus niger and Rhizopus nigricans. Besides, F2 exhibited relatively high stable property even at high temperature, acid and metal ion solutions. The findings of this work suggest the possibility of employing C. oleifera meal as an attractive source of health-promoting compounds, and at the same time facilitate its high-value reuse and reduction of environmental burden. Springer Berlin Heidelberg 2020-08-09 2020 /pmc/articles/PMC7415335/ /pubmed/32837313 http://dx.doi.org/10.1007/s00217-020-03582-0 Text en © Springer-Verlag GmbH Germany, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Paper
Qiu, Yuanxin
He, Di
Yang, Jingxian
Ma, Lukai
Zhu, Kaiqi
Cao, Yong
Kaempferol separated from Camellia oleifera meal by high-speed countercurrent chromatography for antibacterial application
title Kaempferol separated from Camellia oleifera meal by high-speed countercurrent chromatography for antibacterial application
title_full Kaempferol separated from Camellia oleifera meal by high-speed countercurrent chromatography for antibacterial application
title_fullStr Kaempferol separated from Camellia oleifera meal by high-speed countercurrent chromatography for antibacterial application
title_full_unstemmed Kaempferol separated from Camellia oleifera meal by high-speed countercurrent chromatography for antibacterial application
title_short Kaempferol separated from Camellia oleifera meal by high-speed countercurrent chromatography for antibacterial application
title_sort kaempferol separated from camellia oleifera meal by high-speed countercurrent chromatography for antibacterial application
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7415335/
https://www.ncbi.nlm.nih.gov/pubmed/32837313
http://dx.doi.org/10.1007/s00217-020-03582-0
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