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Genome mining of a fungal endophyte of Taxus yunnanensis (Chinese yew) leads to the discovery of a novel azaphilone polyketide, lijiquinone
Genome mining of Ascomycete sp. F53 (F53), a fungal endophyte of the traditional Chinese medicinal plant Taxus yunnanensis (Chinese yew), revealed 35 putative specialized metabolite biosynthesis gene clusters, one of which encodes a rarely seen tandem polyketide synthase pathway with close homology...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7415360/ https://www.ncbi.nlm.nih.gov/pubmed/32281262 http://dx.doi.org/10.1111/1751-7915.13568 |
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author | Cain, Jesse W. Miller, Kristin I. Kalaitzis, John A. Chau, Rocky Neilan, Brett A. |
author_facet | Cain, Jesse W. Miller, Kristin I. Kalaitzis, John A. Chau, Rocky Neilan, Brett A. |
author_sort | Cain, Jesse W. |
collection | PubMed |
description | Genome mining of Ascomycete sp. F53 (F53), a fungal endophyte of the traditional Chinese medicinal plant Taxus yunnanensis (Chinese yew), revealed 35 putative specialized metabolite biosynthesis gene clusters, one of which encodes a rarely seen tandem polyketide synthase pathway with close homology to azaphilone biosynthesis pathways. A novel compound, lijiquinone 1, was subsequently isolated from F53 and structurally and functionally characterized. The m/z 385 [M + H(+)](+) compound, comprised of a cyclohexenone side group attached to a core bicyclic ring, displayed cytotoxicity against human myeloma cells (IC(50) = 129 μM), as well as antifungal activity against Candida albicans (IC(50) = 79 μM) and Cryptococcus albidus (IC(50) = 141 μM). Our results suggest that enzymes encoded on the lij gene cluster are responsible for the synthesis of 1 and that the medicinal properties of T. yunnanensis could be partially mediated by this novel azaphilone. This study highlights the utility of combining traditional knowledge with contemporary genomic approaches for the discovery of new bioactive compounds. |
format | Online Article Text |
id | pubmed-7415360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74153602020-08-10 Genome mining of a fungal endophyte of Taxus yunnanensis (Chinese yew) leads to the discovery of a novel azaphilone polyketide, lijiquinone Cain, Jesse W. Miller, Kristin I. Kalaitzis, John A. Chau, Rocky Neilan, Brett A. Microb Biotechnol Research Articles Genome mining of Ascomycete sp. F53 (F53), a fungal endophyte of the traditional Chinese medicinal plant Taxus yunnanensis (Chinese yew), revealed 35 putative specialized metabolite biosynthesis gene clusters, one of which encodes a rarely seen tandem polyketide synthase pathway with close homology to azaphilone biosynthesis pathways. A novel compound, lijiquinone 1, was subsequently isolated from F53 and structurally and functionally characterized. The m/z 385 [M + H(+)](+) compound, comprised of a cyclohexenone side group attached to a core bicyclic ring, displayed cytotoxicity against human myeloma cells (IC(50) = 129 μM), as well as antifungal activity against Candida albicans (IC(50) = 79 μM) and Cryptococcus albidus (IC(50) = 141 μM). Our results suggest that enzymes encoded on the lij gene cluster are responsible for the synthesis of 1 and that the medicinal properties of T. yunnanensis could be partially mediated by this novel azaphilone. This study highlights the utility of combining traditional knowledge with contemporary genomic approaches for the discovery of new bioactive compounds. John Wiley and Sons Inc. 2020-04-12 /pmc/articles/PMC7415360/ /pubmed/32281262 http://dx.doi.org/10.1111/1751-7915.13568 Text en © 2020 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Cain, Jesse W. Miller, Kristin I. Kalaitzis, John A. Chau, Rocky Neilan, Brett A. Genome mining of a fungal endophyte of Taxus yunnanensis (Chinese yew) leads to the discovery of a novel azaphilone polyketide, lijiquinone |
title | Genome mining of a fungal endophyte of Taxus yunnanensis (Chinese yew) leads to the discovery of a novel azaphilone polyketide, lijiquinone |
title_full | Genome mining of a fungal endophyte of Taxus yunnanensis (Chinese yew) leads to the discovery of a novel azaphilone polyketide, lijiquinone |
title_fullStr | Genome mining of a fungal endophyte of Taxus yunnanensis (Chinese yew) leads to the discovery of a novel azaphilone polyketide, lijiquinone |
title_full_unstemmed | Genome mining of a fungal endophyte of Taxus yunnanensis (Chinese yew) leads to the discovery of a novel azaphilone polyketide, lijiquinone |
title_short | Genome mining of a fungal endophyte of Taxus yunnanensis (Chinese yew) leads to the discovery of a novel azaphilone polyketide, lijiquinone |
title_sort | genome mining of a fungal endophyte of taxus yunnanensis (chinese yew) leads to the discovery of a novel azaphilone polyketide, lijiquinone |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7415360/ https://www.ncbi.nlm.nih.gov/pubmed/32281262 http://dx.doi.org/10.1111/1751-7915.13568 |
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