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Viral Load Kinetics of SARS-CoV-2 Infection in Saliva in Korean Patients: a Prospective Multi-center Comparative Study

BACKGROUND: This study was performed to compare the viral load and kinetics of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in saliva with those in standard nasopharyngeal/oropharyngeal (NP/OP) swabs. METHODS: Fifteen patients with SARS-CoV-2 infection from four hospitals were prospe...

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Autores principales: Kim, Seong Eun, Lee, Ji Yeon, Lee, Ahrang, Kim, Soosung, Park, Kyung-Hwa, Jung, Sook-In, Kang, Seung-Ji, Oh, Tae Hoon, Kim, Uh Jin, Lee, Seung Yeob, Kee, Seung-Jung, Jang, Hee-Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7415999/
https://www.ncbi.nlm.nih.gov/pubmed/32776725
http://dx.doi.org/10.3346/jkms.2020.35.e287
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author Kim, Seong Eun
Lee, Ji Yeon
Lee, Ahrang
Kim, Soosung
Park, Kyung-Hwa
Jung, Sook-In
Kang, Seung-Ji
Oh, Tae Hoon
Kim, Uh Jin
Lee, Seung Yeob
Kee, Seung-Jung
Jang, Hee-Chang
author_facet Kim, Seong Eun
Lee, Ji Yeon
Lee, Ahrang
Kim, Soosung
Park, Kyung-Hwa
Jung, Sook-In
Kang, Seung-Ji
Oh, Tae Hoon
Kim, Uh Jin
Lee, Seung Yeob
Kee, Seung-Jung
Jang, Hee-Chang
author_sort Kim, Seong Eun
collection PubMed
description BACKGROUND: This study was performed to compare the viral load and kinetics of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in saliva with those in standard nasopharyngeal/oropharyngeal (NP/OP) swabs. METHODS: Fifteen patients with SARS-CoV-2 infection from four hospitals were prospectively enrolled and matched samples of nasopharyngeal/oropharyngeal swabs and saliva were collected at Day 1 of admission and every other day till consequently negative for two times. Real-time reverse transcription polymerase chain reaction (rRT-PCR) was performed to detect the envelope (E) and RNA-dependent RNA polymerase (RdRP) genes. RESULTS: The cycle threshold values of saliva were comparable to those of NP/OP swabs overall (P = 0.720, Mann–Whitney U test). However, the overall sensitivity of rRT-PCR using saliva was 64% (34/53), which is lower than the 77% (41/53) using NP/OP swabs. The sensitivity of rRT-PCR using saliva was especially lower in early stage of symptom onset (1–5 days; 8/15; 53%) and in patients who did not have sputum (12/22; 55%). CONCLUSION: Saliva sample itself is not appropriate for initial diagnosis of coronavirus disease 2019 (COVID-19) to replace NP/OP swabs, especially for the person who does not produce sputum. COVID-19 cannot be excluded when the test using saliva is negative, and it is necessary to retest using NP/OP swabs.
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spelling pubmed-74159992020-08-17 Viral Load Kinetics of SARS-CoV-2 Infection in Saliva in Korean Patients: a Prospective Multi-center Comparative Study Kim, Seong Eun Lee, Ji Yeon Lee, Ahrang Kim, Soosung Park, Kyung-Hwa Jung, Sook-In Kang, Seung-Ji Oh, Tae Hoon Kim, Uh Jin Lee, Seung Yeob Kee, Seung-Jung Jang, Hee-Chang J Korean Med Sci Original Article BACKGROUND: This study was performed to compare the viral load and kinetics of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in saliva with those in standard nasopharyngeal/oropharyngeal (NP/OP) swabs. METHODS: Fifteen patients with SARS-CoV-2 infection from four hospitals were prospectively enrolled and matched samples of nasopharyngeal/oropharyngeal swabs and saliva were collected at Day 1 of admission and every other day till consequently negative for two times. Real-time reverse transcription polymerase chain reaction (rRT-PCR) was performed to detect the envelope (E) and RNA-dependent RNA polymerase (RdRP) genes. RESULTS: The cycle threshold values of saliva were comparable to those of NP/OP swabs overall (P = 0.720, Mann–Whitney U test). However, the overall sensitivity of rRT-PCR using saliva was 64% (34/53), which is lower than the 77% (41/53) using NP/OP swabs. The sensitivity of rRT-PCR using saliva was especially lower in early stage of symptom onset (1–5 days; 8/15; 53%) and in patients who did not have sputum (12/22; 55%). CONCLUSION: Saliva sample itself is not appropriate for initial diagnosis of coronavirus disease 2019 (COVID-19) to replace NP/OP swabs, especially for the person who does not produce sputum. COVID-19 cannot be excluded when the test using saliva is negative, and it is necessary to retest using NP/OP swabs. The Korean Academy of Medical Sciences 2020-08-05 /pmc/articles/PMC7415999/ /pubmed/32776725 http://dx.doi.org/10.3346/jkms.2020.35.e287 Text en © 2020 The Korean Academy of Medical Sciences. https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Seong Eun
Lee, Ji Yeon
Lee, Ahrang
Kim, Soosung
Park, Kyung-Hwa
Jung, Sook-In
Kang, Seung-Ji
Oh, Tae Hoon
Kim, Uh Jin
Lee, Seung Yeob
Kee, Seung-Jung
Jang, Hee-Chang
Viral Load Kinetics of SARS-CoV-2 Infection in Saliva in Korean Patients: a Prospective Multi-center Comparative Study
title Viral Load Kinetics of SARS-CoV-2 Infection in Saliva in Korean Patients: a Prospective Multi-center Comparative Study
title_full Viral Load Kinetics of SARS-CoV-2 Infection in Saliva in Korean Patients: a Prospective Multi-center Comparative Study
title_fullStr Viral Load Kinetics of SARS-CoV-2 Infection in Saliva in Korean Patients: a Prospective Multi-center Comparative Study
title_full_unstemmed Viral Load Kinetics of SARS-CoV-2 Infection in Saliva in Korean Patients: a Prospective Multi-center Comparative Study
title_short Viral Load Kinetics of SARS-CoV-2 Infection in Saliva in Korean Patients: a Prospective Multi-center Comparative Study
title_sort viral load kinetics of sars-cov-2 infection in saliva in korean patients: a prospective multi-center comparative study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7415999/
https://www.ncbi.nlm.nih.gov/pubmed/32776725
http://dx.doi.org/10.3346/jkms.2020.35.e287
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