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Mast Cell Deficiency Protects Mice from Surgery-Induced Neuroinflammation

Neuroinflammation plays a key role in the occurrence and development of neurodegenerative diseases. Microglia, the resident immune cells in the brain, have been recognized to contribute to neuroinflammation. Previous studies have shown that activated mast cells may be involved in surgery-induced neu...

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Autores principales: Zhang, Xiang, Dong, Hongquan, Wang, Fei, Zhang, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416247/
https://www.ncbi.nlm.nih.gov/pubmed/32801993
http://dx.doi.org/10.1155/2020/1921826
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author Zhang, Xiang
Dong, Hongquan
Wang, Fei
Zhang, Jun
author_facet Zhang, Xiang
Dong, Hongquan
Wang, Fei
Zhang, Jun
author_sort Zhang, Xiang
collection PubMed
description Neuroinflammation plays a key role in the occurrence and development of neurodegenerative diseases. Microglia, the resident immune cells in the brain, have been recognized to contribute to neuroinflammation. Previous studies have shown that activated mast cells may be involved in surgery-induced neuroinflammation and neuronal apoptosis by using pharmacological methods. This study is aimed at ascertaining the exactly role of mast cells on neuroinflammation with the mast cell-deficient mice. Adult male C57BL6/J wild-type (WT) and mast cell-deficient (C57BL6/J KitWsh/Wsh (Wsh)) mice underwent tibial fracture surgery. Blood-brain barrier (BBB) breakdown, microglial activation, and neuroinflammatory levels were examined at 1 day after surgery. Surgery-induced BBB breakdown, microglial activation, and neuroinflammatory levels were significantly, pharmacologically reduced using a mast cell stabilizer, cromolyn sodium in WT mice (P < 0.05). These results were reproduced with mast cell deficiency. WT mice administered intraventricularly with cromolyn exhibited reduced BBB breakdown, microglial activation, and neuroinflammatory levels versus vehicle (P < 0.05). But there was no effect of cromolyn versus vehicle in Wsh mice, clarifying the specificity of cromolyn on brain mast cells. These findings demonstrated that activated mast cells promote surgery-induced BBB breakdown and neuroinflammation in mice, and open up a new therapeutic target for neuroinflammation-related diseases.
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spelling pubmed-74162472020-08-14 Mast Cell Deficiency Protects Mice from Surgery-Induced Neuroinflammation Zhang, Xiang Dong, Hongquan Wang, Fei Zhang, Jun Mediators Inflamm Research Article Neuroinflammation plays a key role in the occurrence and development of neurodegenerative diseases. Microglia, the resident immune cells in the brain, have been recognized to contribute to neuroinflammation. Previous studies have shown that activated mast cells may be involved in surgery-induced neuroinflammation and neuronal apoptosis by using pharmacological methods. This study is aimed at ascertaining the exactly role of mast cells on neuroinflammation with the mast cell-deficient mice. Adult male C57BL6/J wild-type (WT) and mast cell-deficient (C57BL6/J KitWsh/Wsh (Wsh)) mice underwent tibial fracture surgery. Blood-brain barrier (BBB) breakdown, microglial activation, and neuroinflammatory levels were examined at 1 day after surgery. Surgery-induced BBB breakdown, microglial activation, and neuroinflammatory levels were significantly, pharmacologically reduced using a mast cell stabilizer, cromolyn sodium in WT mice (P < 0.05). These results were reproduced with mast cell deficiency. WT mice administered intraventricularly with cromolyn exhibited reduced BBB breakdown, microglial activation, and neuroinflammatory levels versus vehicle (P < 0.05). But there was no effect of cromolyn versus vehicle in Wsh mice, clarifying the specificity of cromolyn on brain mast cells. These findings demonstrated that activated mast cells promote surgery-induced BBB breakdown and neuroinflammation in mice, and open up a new therapeutic target for neuroinflammation-related diseases. Hindawi 2020-08-01 /pmc/articles/PMC7416247/ /pubmed/32801993 http://dx.doi.org/10.1155/2020/1921826 Text en Copyright © 2020 Xiang Zhang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Xiang
Dong, Hongquan
Wang, Fei
Zhang, Jun
Mast Cell Deficiency Protects Mice from Surgery-Induced Neuroinflammation
title Mast Cell Deficiency Protects Mice from Surgery-Induced Neuroinflammation
title_full Mast Cell Deficiency Protects Mice from Surgery-Induced Neuroinflammation
title_fullStr Mast Cell Deficiency Protects Mice from Surgery-Induced Neuroinflammation
title_full_unstemmed Mast Cell Deficiency Protects Mice from Surgery-Induced Neuroinflammation
title_short Mast Cell Deficiency Protects Mice from Surgery-Induced Neuroinflammation
title_sort mast cell deficiency protects mice from surgery-induced neuroinflammation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416247/
https://www.ncbi.nlm.nih.gov/pubmed/32801993
http://dx.doi.org/10.1155/2020/1921826
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