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The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats

BACKGROUND: The precise mechanisms of nerve regeneration remain unclear. The potential of facial nerve regeneration and probable mechanisms involved following chronic facial nerve injury should be further studied. METHODS: Adult male Wistar rats were used to model either (i) facial nerve injury (axo...

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Autores principales: Ni, Yusu, Chen, Diyan, Jiang, Yi, Qiu, Danhong, Li, Wen, Li, Huawei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416273/
https://www.ncbi.nlm.nih.gov/pubmed/32802043
http://dx.doi.org/10.1155/2020/8884511
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author Ni, Yusu
Chen, Diyan
Jiang, Yi
Qiu, Danhong
Li, Wen
Li, Huawei
author_facet Ni, Yusu
Chen, Diyan
Jiang, Yi
Qiu, Danhong
Li, Wen
Li, Huawei
author_sort Ni, Yusu
collection PubMed
description BACKGROUND: The precise mechanisms of nerve regeneration remain unclear. The potential of facial nerve regeneration and probable mechanisms involved following chronic facial nerve injury should be further studied. METHODS: Adult male Wistar rats were used to model either (i) facial nerve injury (axotomy) or (ii) reinjury (chronic axotomy followed by a second axotomy within 5 months). The rats were housed in the animal facility of the Eye and ENT Hospital of Shanghai Medical School, Fudan University (Shanghai, China). Expression of Shh (sonic hedgehog) and growth-associated protein 43 (GAP43, a neuronal marker) was detected in bilateral facial nuclei using reverse transcriptase PCR, western blotting analysis, and immunohistochemistry. The number of surviving motoneurons was quantified, and facial nerve regeneration was examined using transmission electron microscopy. RESULTS: Reinjury of the facial nerve 12 weeks after the first axotomy resulted in upregulation of GAP43 mRNA and protein expression in neurons ipsilateral to the axotomy; immunohistochemistry revealed that Shh expression was higher compared with control side facial nuclei at the same time point. GAP43 expression subsequently decreased. CONCLUSION: The greatest regeneration potential of the facial nerve occurred within 5 months following chronic axotomy in rats, and regeneration may involve the Shh signaling pathway.
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spelling pubmed-74162732020-08-14 The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats Ni, Yusu Chen, Diyan Jiang, Yi Qiu, Danhong Li, Wen Li, Huawei Neural Plast Research Article BACKGROUND: The precise mechanisms of nerve regeneration remain unclear. The potential of facial nerve regeneration and probable mechanisms involved following chronic facial nerve injury should be further studied. METHODS: Adult male Wistar rats were used to model either (i) facial nerve injury (axotomy) or (ii) reinjury (chronic axotomy followed by a second axotomy within 5 months). The rats were housed in the animal facility of the Eye and ENT Hospital of Shanghai Medical School, Fudan University (Shanghai, China). Expression of Shh (sonic hedgehog) and growth-associated protein 43 (GAP43, a neuronal marker) was detected in bilateral facial nuclei using reverse transcriptase PCR, western blotting analysis, and immunohistochemistry. The number of surviving motoneurons was quantified, and facial nerve regeneration was examined using transmission electron microscopy. RESULTS: Reinjury of the facial nerve 12 weeks after the first axotomy resulted in upregulation of GAP43 mRNA and protein expression in neurons ipsilateral to the axotomy; immunohistochemistry revealed that Shh expression was higher compared with control side facial nuclei at the same time point. GAP43 expression subsequently decreased. CONCLUSION: The greatest regeneration potential of the facial nerve occurred within 5 months following chronic axotomy in rats, and regeneration may involve the Shh signaling pathway. Hindawi 2020-08-01 /pmc/articles/PMC7416273/ /pubmed/32802043 http://dx.doi.org/10.1155/2020/8884511 Text en Copyright © 2020 Yusu Ni et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ni, Yusu
Chen, Diyan
Jiang, Yi
Qiu, Danhong
Li, Wen
Li, Huawei
The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats
title The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats
title_full The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats
title_fullStr The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats
title_full_unstemmed The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats
title_short The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats
title_sort regenerative potential of facial nerve motoneurons following chronic axotomy in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416273/
https://www.ncbi.nlm.nih.gov/pubmed/32802043
http://dx.doi.org/10.1155/2020/8884511
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