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Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model

BACKGROUND: Glioblastoma (GBM) are highly heterogeneous on the cellular and molecular basis. It has been proposed that glutamine metabolism of primary cells established from human tumors discriminates aggressive mesenchymal GBM subtype to other subtypes. METHODS: To study glutamine metabolism in viv...

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Autores principales: Oizel, Kristell, Yang, Chendong, Renoult, Ophelie, Gautier, Fabien, Do, Quyen N., Joalland, Noemie, Gao, Xiaofei, Ko, Bookyung, Vallette, François, Ge, Woo-Ping, Paris, François, DeBerardinis, Ralph J., Pecqueur, Claire
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416393/
https://www.ncbi.nlm.nih.gov/pubmed/32789014
http://dx.doi.org/10.1186/s40170-020-00215-8
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author Oizel, Kristell
Yang, Chendong
Renoult, Ophelie
Gautier, Fabien
Do, Quyen N.
Joalland, Noemie
Gao, Xiaofei
Ko, Bookyung
Vallette, François
Ge, Woo-Ping
Paris, François
DeBerardinis, Ralph J.
Pecqueur, Claire
author_facet Oizel, Kristell
Yang, Chendong
Renoult, Ophelie
Gautier, Fabien
Do, Quyen N.
Joalland, Noemie
Gao, Xiaofei
Ko, Bookyung
Vallette, François
Ge, Woo-Ping
Paris, François
DeBerardinis, Ralph J.
Pecqueur, Claire
author_sort Oizel, Kristell
collection PubMed
description BACKGROUND: Glioblastoma (GBM) are highly heterogeneous on the cellular and molecular basis. It has been proposed that glutamine metabolism of primary cells established from human tumors discriminates aggressive mesenchymal GBM subtype to other subtypes. METHODS: To study glutamine metabolism in vivo, we used a human orthotopic mouse model for GBM. Tumors evolving from the implanted primary GBM cells expressing different molecular signatures were analyzed using mass spectrometry for their metabolite pools and enrichment in carbon 13 ((13)C) after (13)C-glutamine infusion. RESULTS: Our results showed that mesenchymal GBM tumors displayed increased glutamine uptake and utilization compared to both control brain tissue and other GBM subtypes. Furthermore, both glutamine synthetase and transglutaminase-2 were expressed accordingly to GBM metabolic phenotypes. CONCLUSION: Thus, our results outline the specific enhanced glutamine flux in vivo of the aggressive mesenchymal GBM subtype.
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spelling pubmed-74163932020-08-11 Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model Oizel, Kristell Yang, Chendong Renoult, Ophelie Gautier, Fabien Do, Quyen N. Joalland, Noemie Gao, Xiaofei Ko, Bookyung Vallette, François Ge, Woo-Ping Paris, François DeBerardinis, Ralph J. Pecqueur, Claire Cancer Metab Research BACKGROUND: Glioblastoma (GBM) are highly heterogeneous on the cellular and molecular basis. It has been proposed that glutamine metabolism of primary cells established from human tumors discriminates aggressive mesenchymal GBM subtype to other subtypes. METHODS: To study glutamine metabolism in vivo, we used a human orthotopic mouse model for GBM. Tumors evolving from the implanted primary GBM cells expressing different molecular signatures were analyzed using mass spectrometry for their metabolite pools and enrichment in carbon 13 ((13)C) after (13)C-glutamine infusion. RESULTS: Our results showed that mesenchymal GBM tumors displayed increased glutamine uptake and utilization compared to both control brain tissue and other GBM subtypes. Furthermore, both glutamine synthetase and transglutaminase-2 were expressed accordingly to GBM metabolic phenotypes. CONCLUSION: Thus, our results outline the specific enhanced glutamine flux in vivo of the aggressive mesenchymal GBM subtype. BioMed Central 2020-08-10 /pmc/articles/PMC7416393/ /pubmed/32789014 http://dx.doi.org/10.1186/s40170-020-00215-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Oizel, Kristell
Yang, Chendong
Renoult, Ophelie
Gautier, Fabien
Do, Quyen N.
Joalland, Noemie
Gao, Xiaofei
Ko, Bookyung
Vallette, François
Ge, Woo-Ping
Paris, François
DeBerardinis, Ralph J.
Pecqueur, Claire
Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model
title Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model
title_full Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model
title_fullStr Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model
title_full_unstemmed Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model
title_short Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model
title_sort glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416393/
https://www.ncbi.nlm.nih.gov/pubmed/32789014
http://dx.doi.org/10.1186/s40170-020-00215-8
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