Isolation of Microglia from Mouse or Human Tissue

Microglia are the innate immune cells of the central nervous system. Although numerous methods have been developed to isolate microglia from the brain, the method of dissociation and isolation can have a profound effect on the function of these highly dynamic cells. Here, we present an optimized pro...

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Autores principales: Bordt, Evan A., Block, Carina L., Petrozziello, Tiziana, Sadri-Vakili, Ghazaleh, Smith, Caroline J., Edlow, Andrea G., Bilbo, Staci D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416840/
https://www.ncbi.nlm.nih.gov/pubmed/32783030
http://dx.doi.org/10.1016/j.xpro.2020.100035
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author Bordt, Evan A.
Block, Carina L.
Petrozziello, Tiziana
Sadri-Vakili, Ghazaleh
Smith, Caroline J.
Edlow, Andrea G.
Bilbo, Staci D.
author_facet Bordt, Evan A.
Block, Carina L.
Petrozziello, Tiziana
Sadri-Vakili, Ghazaleh
Smith, Caroline J.
Edlow, Andrea G.
Bilbo, Staci D.
author_sort Bordt, Evan A.
collection PubMed
description Microglia are the innate immune cells of the central nervous system. Although numerous methods have been developed to isolate microglia from the brain, the method of dissociation and isolation can have a profound effect on the function of these highly dynamic cells. Here, we present an optimized protocol to isolate CD11b+ cells (microglia) from mouse or human brain tissue using magnetic bead columns. Isolated microglia can be used to model diseases with neuroinflammatory components for potential therapeutic discoveries. For complete details on the use and execution of this protocol, please refer to Hanamsagar et al., 2017, Rivera et al., 2019, and Edlow et al. (2019)
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spelling pubmed-74168402020-08-10 Isolation of Microglia from Mouse or Human Tissue Bordt, Evan A. Block, Carina L. Petrozziello, Tiziana Sadri-Vakili, Ghazaleh Smith, Caroline J. Edlow, Andrea G. Bilbo, Staci D. STAR Protoc Protocol Microglia are the innate immune cells of the central nervous system. Although numerous methods have been developed to isolate microglia from the brain, the method of dissociation and isolation can have a profound effect on the function of these highly dynamic cells. Here, we present an optimized protocol to isolate CD11b+ cells (microglia) from mouse or human brain tissue using magnetic bead columns. Isolated microglia can be used to model diseases with neuroinflammatory components for potential therapeutic discoveries. For complete details on the use and execution of this protocol, please refer to Hanamsagar et al., 2017, Rivera et al., 2019, and Edlow et al. (2019) Elsevier 2020-06-03 /pmc/articles/PMC7416840/ /pubmed/32783030 http://dx.doi.org/10.1016/j.xpro.2020.100035 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Bordt, Evan A.
Block, Carina L.
Petrozziello, Tiziana
Sadri-Vakili, Ghazaleh
Smith, Caroline J.
Edlow, Andrea G.
Bilbo, Staci D.
Isolation of Microglia from Mouse or Human Tissue
title Isolation of Microglia from Mouse or Human Tissue
title_full Isolation of Microglia from Mouse or Human Tissue
title_fullStr Isolation of Microglia from Mouse or Human Tissue
title_full_unstemmed Isolation of Microglia from Mouse or Human Tissue
title_short Isolation of Microglia from Mouse or Human Tissue
title_sort isolation of microglia from mouse or human tissue
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416840/
https://www.ncbi.nlm.nih.gov/pubmed/32783030
http://dx.doi.org/10.1016/j.xpro.2020.100035
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