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Diagnosis of acute graft-versus-host disease in the gastrointestinal tract of patients undergoing allogeneic hematopoietic stem cell transplantation. A descriptive and critical study of diagnostic tests()
INTRODUCTION: Acute graft-versus-host disease (GVHD) is one of the major causes of morbidity and mortality in patients undergoing allogeneic hematopoietic stem cell transplantation (AHSCT) and has become the subject of several studies to understand and treat it. OBJECTIVE: This study does a descript...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Sociedade Brasileira de Hematologia e Hemoterapia
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417442/ https://www.ncbi.nlm.nih.gov/pubmed/31780391 http://dx.doi.org/10.1016/j.htct.2019.07.002 |
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author | Borges, Leticia Rocha Vilela, Eduardo E. Ferrari, Maria de Lourdes A. Cunha, Aloisio S. Vasconcelos, Anilton C. Torres, Henrique O.G. |
author_facet | Borges, Leticia Rocha Vilela, Eduardo E. Ferrari, Maria de Lourdes A. Cunha, Aloisio S. Vasconcelos, Anilton C. Torres, Henrique O.G. |
author_sort | Borges, Leticia Rocha |
collection | PubMed |
description | INTRODUCTION: Acute graft-versus-host disease (GVHD) is one of the major causes of morbidity and mortality in patients undergoing allogeneic hematopoietic stem cell transplantation (AHSCT) and has become the subject of several studies to understand and treat it. OBJECTIVE: This study does a descriptive analysis of the apoptotic index (AI) evaluation and intestinal permeability (IP) alterations in association with the clinical, endoscopic and histopathological data on patients undergoing AHSCT, with emphasis on acute intestinal graft-versus-host disease (GVHD) diagnosis. METHODS: Thirty-one patients were divided into two groups—one of patients with a clinical GVHD diagnosis and one of those without GVHD diagnosis. RESULTS: Thirteen deaths (41.9%) occurred during the study period, thereby reaffirming the severity of the alterations found in the patients. Fifteen patients subjected to 21 esophagogastroduodenoscopy procedures prior to D + 90 post-transplant had visible endoscopic alterations and 19 biopsies revealed histological alterations to the stomach and duodenum. Higher apoptotic indices, not reaching statistical significance, were observed in patients who died of graft versus host disease (GVHD), in the more acute forms of GVHD and where clinical GVHD was present. The intestinal permeability evaluation was performed on nine patients able to undergo it in the three proposed study periods, which showed alterations, some of which were pronounced even during pre-transplant and, therefore, the pre-conditioning phase. CONCLUSION: Clinical judgment remains a fundamental tool in the diagnosis of GVHD. This study points to the known limitations of traditional diagnostic aids (endoscopy and histology) and points to new methods not usually employed in clinical practice. |
format | Online Article Text |
id | pubmed-7417442 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Sociedade Brasileira de Hematologia e Hemoterapia |
record_format | MEDLINE/PubMed |
spelling | pubmed-74174422020-08-14 Diagnosis of acute graft-versus-host disease in the gastrointestinal tract of patients undergoing allogeneic hematopoietic stem cell transplantation. A descriptive and critical study of diagnostic tests() Borges, Leticia Rocha Vilela, Eduardo E. Ferrari, Maria de Lourdes A. Cunha, Aloisio S. Vasconcelos, Anilton C. Torres, Henrique O.G. Hematol Transfus Cell Ther Original Article INTRODUCTION: Acute graft-versus-host disease (GVHD) is one of the major causes of morbidity and mortality in patients undergoing allogeneic hematopoietic stem cell transplantation (AHSCT) and has become the subject of several studies to understand and treat it. OBJECTIVE: This study does a descriptive analysis of the apoptotic index (AI) evaluation and intestinal permeability (IP) alterations in association with the clinical, endoscopic and histopathological data on patients undergoing AHSCT, with emphasis on acute intestinal graft-versus-host disease (GVHD) diagnosis. METHODS: Thirty-one patients were divided into two groups—one of patients with a clinical GVHD diagnosis and one of those without GVHD diagnosis. RESULTS: Thirteen deaths (41.9%) occurred during the study period, thereby reaffirming the severity of the alterations found in the patients. Fifteen patients subjected to 21 esophagogastroduodenoscopy procedures prior to D + 90 post-transplant had visible endoscopic alterations and 19 biopsies revealed histological alterations to the stomach and duodenum. Higher apoptotic indices, not reaching statistical significance, were observed in patients who died of graft versus host disease (GVHD), in the more acute forms of GVHD and where clinical GVHD was present. The intestinal permeability evaluation was performed on nine patients able to undergo it in the three proposed study periods, which showed alterations, some of which were pronounced even during pre-transplant and, therefore, the pre-conditioning phase. CONCLUSION: Clinical judgment remains a fundamental tool in the diagnosis of GVHD. This study points to the known limitations of traditional diagnostic aids (endoscopy and histology) and points to new methods not usually employed in clinical practice. Sociedade Brasileira de Hematologia e Hemoterapia 2020 2019-09-10 /pmc/articles/PMC7417442/ /pubmed/31780391 http://dx.doi.org/10.1016/j.htct.2019.07.002 Text en © 2019 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Borges, Leticia Rocha Vilela, Eduardo E. Ferrari, Maria de Lourdes A. Cunha, Aloisio S. Vasconcelos, Anilton C. Torres, Henrique O.G. Diagnosis of acute graft-versus-host disease in the gastrointestinal tract of patients undergoing allogeneic hematopoietic stem cell transplantation. A descriptive and critical study of diagnostic tests() |
title | Diagnosis of acute graft-versus-host disease in the gastrointestinal tract of patients undergoing allogeneic hematopoietic stem cell transplantation. A descriptive and critical study of diagnostic tests() |
title_full | Diagnosis of acute graft-versus-host disease in the gastrointestinal tract of patients undergoing allogeneic hematopoietic stem cell transplantation. A descriptive and critical study of diagnostic tests() |
title_fullStr | Diagnosis of acute graft-versus-host disease in the gastrointestinal tract of patients undergoing allogeneic hematopoietic stem cell transplantation. A descriptive and critical study of diagnostic tests() |
title_full_unstemmed | Diagnosis of acute graft-versus-host disease in the gastrointestinal tract of patients undergoing allogeneic hematopoietic stem cell transplantation. A descriptive and critical study of diagnostic tests() |
title_short | Diagnosis of acute graft-versus-host disease in the gastrointestinal tract of patients undergoing allogeneic hematopoietic stem cell transplantation. A descriptive and critical study of diagnostic tests() |
title_sort | diagnosis of acute graft-versus-host disease in the gastrointestinal tract of patients undergoing allogeneic hematopoietic stem cell transplantation. a descriptive and critical study of diagnostic tests() |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417442/ https://www.ncbi.nlm.nih.gov/pubmed/31780391 http://dx.doi.org/10.1016/j.htct.2019.07.002 |
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