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In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate
BACKGROUND: Anthropogenic activity, climate change, pollution, and exploitation of natural resources are some reasons that cause threatening of plant diversity. Silene schimperiana is an endangered plant species in Egypt and is endemic to the high mountain of Saint Katherine Protected Area in southe...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417468/ https://www.ncbi.nlm.nih.gov/pubmed/32778978 http://dx.doi.org/10.1186/s43141-020-00052-8 |
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author | Ghareb, Heba El-Sayed Ibrahim, Shafik Darwish Hegazi, Ghada Abd El-Moneim |
author_facet | Ghareb, Heba El-Sayed Ibrahim, Shafik Darwish Hegazi, Ghada Abd El-Moneim |
author_sort | Ghareb, Heba El-Sayed |
collection | PubMed |
description | BACKGROUND: Anthropogenic activity, climate change, pollution, and exploitation of natural resources are some reasons that cause threatening of plant diversity. Silene schimperiana is an endangered plant species in Egypt and is endemic to the high mountain of Saint Katherine Protected Area in southern Sinai. The purpose of the study was the ex situ conservation of Silene schimperiana through in vitro propagation and DNA barcode analysis. RESULTS: To develop an efficient ex situ conservation program of the plant, in vitro propagation protocol has been achieved from shoot tip and stem nodal segment explants of in vitro germinated seedlings. Explants were established in vitro on Murashige and Skoog (MS) medium supplemented with 2.89 μM gibberellic acid (GA(3))(,) 1.08 μM α-naphthaleneacetic acid (NAA), and 1.16 μM kinetin (Kin). The highest number of axillary shoots (9.27) was obtained when they were transferred to MS medium supplemented with 4.48 μM 6-benzyl adenine (BA). Hundred percent of multiple axillary shoots were rooted on quarter-strength MS medium supplemented with 4.92 μM indole-3-butyric acid (IBA) and 10.75 μM NAA. Rooted plants were transferred to pots containing a soil-peat mixture (1: 2 v/v) and successfully acclimatized in the greenhouse. Plant identification is a crucial aspect to understand and conserve plant diversity from extinction. DNA barcode analysis of Silene schimperiana was carried out using two chloroplast DNA markers (cpDNA): 1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) and RNA polymerase subunit (rpoC1) and a nuclear ribosome DNA marker (ncDNA), internal transcribed spacer (ITS). Phylogenetic analysis revealed a successful identification of Silene schimperiana on the species and genus levels and supported the inclusion of Silene schimperiana in genus Silene. CONCLUSIONS: In this study, a relevant in vitro propagation method was established to facilitate the recovery of Silene schimperiana, in addition to DNA barcoding of the plant as a tool for effective management and conservation of plant genetic resources. |
format | Online Article Text |
id | pubmed-7417468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-74174682020-08-17 In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate Ghareb, Heba El-Sayed Ibrahim, Shafik Darwish Hegazi, Ghada Abd El-Moneim J Genet Eng Biotechnol Research BACKGROUND: Anthropogenic activity, climate change, pollution, and exploitation of natural resources are some reasons that cause threatening of plant diversity. Silene schimperiana is an endangered plant species in Egypt and is endemic to the high mountain of Saint Katherine Protected Area in southern Sinai. The purpose of the study was the ex situ conservation of Silene schimperiana through in vitro propagation and DNA barcode analysis. RESULTS: To develop an efficient ex situ conservation program of the plant, in vitro propagation protocol has been achieved from shoot tip and stem nodal segment explants of in vitro germinated seedlings. Explants were established in vitro on Murashige and Skoog (MS) medium supplemented with 2.89 μM gibberellic acid (GA(3))(,) 1.08 μM α-naphthaleneacetic acid (NAA), and 1.16 μM kinetin (Kin). The highest number of axillary shoots (9.27) was obtained when they were transferred to MS medium supplemented with 4.48 μM 6-benzyl adenine (BA). Hundred percent of multiple axillary shoots were rooted on quarter-strength MS medium supplemented with 4.92 μM indole-3-butyric acid (IBA) and 10.75 μM NAA. Rooted plants were transferred to pots containing a soil-peat mixture (1: 2 v/v) and successfully acclimatized in the greenhouse. Plant identification is a crucial aspect to understand and conserve plant diversity from extinction. DNA barcode analysis of Silene schimperiana was carried out using two chloroplast DNA markers (cpDNA): 1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) and RNA polymerase subunit (rpoC1) and a nuclear ribosome DNA marker (ncDNA), internal transcribed spacer (ITS). Phylogenetic analysis revealed a successful identification of Silene schimperiana on the species and genus levels and supported the inclusion of Silene schimperiana in genus Silene. CONCLUSIONS: In this study, a relevant in vitro propagation method was established to facilitate the recovery of Silene schimperiana, in addition to DNA barcoding of the plant as a tool for effective management and conservation of plant genetic resources. Springer Berlin Heidelberg 2020-08-10 /pmc/articles/PMC7417468/ /pubmed/32778978 http://dx.doi.org/10.1186/s43141-020-00052-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Ghareb, Heba El-Sayed Ibrahim, Shafik Darwish Hegazi, Ghada Abd El-Moneim In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate |
title | In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate |
title_full | In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate |
title_fullStr | In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate |
title_full_unstemmed | In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate |
title_short | In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate |
title_sort | in vitro propagation and dna barcode analysis of the endangered silene schimperiana in saint katherine protectorate |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417468/ https://www.ncbi.nlm.nih.gov/pubmed/32778978 http://dx.doi.org/10.1186/s43141-020-00052-8 |
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