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The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris

Candida auris has emerged as a medically important pathogen with considerable resistance to antifungal agents. The ability to produce biofilms is an important pathogenicity feature of this species that aids escape of host immune responses and antimicrobial agents. The objective of this study was to...

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Autores principales: Rossoni, Rodnei Dennis, de Barros, Patrícia Pimentel, Mendonça, Iatã do Carmo, Medina, Rebeca Previate, Silva, Dulce Helena Siqueira, Fuchs, Beth Burgwyn, Junqueira, Juliana Campos, Mylonakis, Eleftherios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417517/
https://www.ncbi.nlm.nih.gov/pubmed/32850495
http://dx.doi.org/10.3389/fcimb.2020.00397
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author Rossoni, Rodnei Dennis
de Barros, Patrícia Pimentel
Mendonça, Iatã do Carmo
Medina, Rebeca Previate
Silva, Dulce Helena Siqueira
Fuchs, Beth Burgwyn
Junqueira, Juliana Campos
Mylonakis, Eleftherios
author_facet Rossoni, Rodnei Dennis
de Barros, Patrícia Pimentel
Mendonça, Iatã do Carmo
Medina, Rebeca Previate
Silva, Dulce Helena Siqueira
Fuchs, Beth Burgwyn
Junqueira, Juliana Campos
Mylonakis, Eleftherios
author_sort Rossoni, Rodnei Dennis
collection PubMed
description Candida auris has emerged as a medically important pathogen with considerable resistance to antifungal agents. The ability to produce biofilms is an important pathogenicity feature of this species that aids escape of host immune responses and antimicrobial agents. The objective of this study was to verify antifungal action using in vitro and in vivo models of the Lactobacillus paracasei 28.4 probiotic cells and postbiotic activity of crude extract (LPCE) and fraction 1 (LPF1), derived from L. paracasei 28.4 supernatant. Both live cells and cells free supernatant of L. paracasei 28.4 inhibited C. auris suggesting probiotic and postbiotic effects. The minimum inhibitory concentration (MIC) for LPCE was 15 mg/mL and ranges from 3.75 to 7.5 mg/mL for LPF1. Killing kinetics determined that after 24 h treatment with LPCE or LPF1 there was a complete reduction of viable C. auris cells compared to fluconazole, which decreased the initial inoculum by 1-logCFU during the same time period. LPCE and LPF1 significantly reduced the biomass (p = 0.0001) and the metabolic activity (p = 0.0001) of C. auris biofilm. There was also a total reduction (~10(8) CFU/mL) in viability of persister C. auris cells after treatment with postbiotic elements (p < 0.0001). In an in vivo study, injection of LPCE and LPF1 into G. mellonella larvae infected with C. auris prolonged survival of these insects compared to a control group (p < 0.05) and elicited immune responses by increasing the number of circulating hemocytes and gene expression of antimicrobial peptide galiomicin. We concluded that the L. paracasei 28.4 cells and postbiotic elements (LPCE and LPF1) have antifungal activity against planktonic cells, biofilms, and persister cells of C. auris. Postbiotic supplementation derived from L. paracasei 28.4 protected G. mellonella infected with C. auris and enhanced its immune status indicating a dual function in modulating a host immune response.
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spelling pubmed-74175172020-08-25 The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris Rossoni, Rodnei Dennis de Barros, Patrícia Pimentel Mendonça, Iatã do Carmo Medina, Rebeca Previate Silva, Dulce Helena Siqueira Fuchs, Beth Burgwyn Junqueira, Juliana Campos Mylonakis, Eleftherios Front Cell Infect Microbiol Cellular and Infection Microbiology Candida auris has emerged as a medically important pathogen with considerable resistance to antifungal agents. The ability to produce biofilms is an important pathogenicity feature of this species that aids escape of host immune responses and antimicrobial agents. The objective of this study was to verify antifungal action using in vitro and in vivo models of the Lactobacillus paracasei 28.4 probiotic cells and postbiotic activity of crude extract (LPCE) and fraction 1 (LPF1), derived from L. paracasei 28.4 supernatant. Both live cells and cells free supernatant of L. paracasei 28.4 inhibited C. auris suggesting probiotic and postbiotic effects. The minimum inhibitory concentration (MIC) for LPCE was 15 mg/mL and ranges from 3.75 to 7.5 mg/mL for LPF1. Killing kinetics determined that after 24 h treatment with LPCE or LPF1 there was a complete reduction of viable C. auris cells compared to fluconazole, which decreased the initial inoculum by 1-logCFU during the same time period. LPCE and LPF1 significantly reduced the biomass (p = 0.0001) and the metabolic activity (p = 0.0001) of C. auris biofilm. There was also a total reduction (~10(8) CFU/mL) in viability of persister C. auris cells after treatment with postbiotic elements (p < 0.0001). In an in vivo study, injection of LPCE and LPF1 into G. mellonella larvae infected with C. auris prolonged survival of these insects compared to a control group (p < 0.05) and elicited immune responses by increasing the number of circulating hemocytes and gene expression of antimicrobial peptide galiomicin. We concluded that the L. paracasei 28.4 cells and postbiotic elements (LPCE and LPF1) have antifungal activity against planktonic cells, biofilms, and persister cells of C. auris. Postbiotic supplementation derived from L. paracasei 28.4 protected G. mellonella infected with C. auris and enhanced its immune status indicating a dual function in modulating a host immune response. Frontiers Media S.A. 2020-08-04 /pmc/articles/PMC7417517/ /pubmed/32850495 http://dx.doi.org/10.3389/fcimb.2020.00397 Text en Copyright © 2020 Rossoni, de Barros, Mendonça, Medina, Silva, Fuchs, Junqueira and Mylonakis. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Rossoni, Rodnei Dennis
de Barros, Patrícia Pimentel
Mendonça, Iatã do Carmo
Medina, Rebeca Previate
Silva, Dulce Helena Siqueira
Fuchs, Beth Burgwyn
Junqueira, Juliana Campos
Mylonakis, Eleftherios
The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris
title The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris
title_full The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris
title_fullStr The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris
title_full_unstemmed The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris
title_short The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris
title_sort postbiotic activity of lactobacillus paracasei 28.4 against candida auris
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417517/
https://www.ncbi.nlm.nih.gov/pubmed/32850495
http://dx.doi.org/10.3389/fcimb.2020.00397
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