Cargando…
Karyotype changes in long-term cultured tick cell lines
Tick cell lines are an easy-to-handle system for the study of viral and bacterial infections and other aspects of tick cellular processes. Tick cell cultures are often continuously cultivated, as freezing can affect their viability. However, the long-term cultivation of tick cells can influence thei...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417564/ https://www.ncbi.nlm.nih.gov/pubmed/32778731 http://dx.doi.org/10.1038/s41598-020-70330-5 |
_version_ | 1783569521820303360 |
---|---|
author | Kotsarenko, Kateryna Vechtova, Pavlina Lieskovska, Jaroslava Füssy, Zoltán Cabral-de-Mello, Diogo C. Rego, Ryan O. M. Alberdi, Pilar Collins, Marisol Bell-Sakyi, Lesley Sterba, Jan Grubhoffer, Libor |
author_facet | Kotsarenko, Kateryna Vechtova, Pavlina Lieskovska, Jaroslava Füssy, Zoltán Cabral-de-Mello, Diogo C. Rego, Ryan O. M. Alberdi, Pilar Collins, Marisol Bell-Sakyi, Lesley Sterba, Jan Grubhoffer, Libor |
author_sort | Kotsarenko, Kateryna |
collection | PubMed |
description | Tick cell lines are an easy-to-handle system for the study of viral and bacterial infections and other aspects of tick cellular processes. Tick cell cultures are often continuously cultivated, as freezing can affect their viability. However, the long-term cultivation of tick cells can influence their genome stability. In the present study, we investigated karyotype and genome size of tick cell lines. Though 16S rDNA sequencing showed the similarity between Ixodes spp. cell lines at different passages, their karyotypes differed from 2n = 28 chromosomes for parental Ixodes spp. ticks, and both increase and decrease in chromosome numbers were observed. For example, the highly passaged Ixodes scapularis cell line ISE18 and Ixodes ricinus cell lines IRE/CTVM19 and IRE/CTVM20 had modal chromosome numbers 48, 23 and 48, respectively. Also, the Ornithodoros moubata cell line OME/CTVM22 had the modal chromosome number 33 instead of 2n = 20 chromosomes for Ornithodoros spp. ticks. All studied tick cell lines had a larger genome size in comparison to the genomes of the parental ticks. Thus, highly passaged tick cell lines can be used for research purposes, but possible differences in encoded genetic information and downstream cellular processes, between different cell populations, should be taken into account. |
format | Online Article Text |
id | pubmed-7417564 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-74175642020-08-11 Karyotype changes in long-term cultured tick cell lines Kotsarenko, Kateryna Vechtova, Pavlina Lieskovska, Jaroslava Füssy, Zoltán Cabral-de-Mello, Diogo C. Rego, Ryan O. M. Alberdi, Pilar Collins, Marisol Bell-Sakyi, Lesley Sterba, Jan Grubhoffer, Libor Sci Rep Article Tick cell lines are an easy-to-handle system for the study of viral and bacterial infections and other aspects of tick cellular processes. Tick cell cultures are often continuously cultivated, as freezing can affect their viability. However, the long-term cultivation of tick cells can influence their genome stability. In the present study, we investigated karyotype and genome size of tick cell lines. Though 16S rDNA sequencing showed the similarity between Ixodes spp. cell lines at different passages, their karyotypes differed from 2n = 28 chromosomes for parental Ixodes spp. ticks, and both increase and decrease in chromosome numbers were observed. For example, the highly passaged Ixodes scapularis cell line ISE18 and Ixodes ricinus cell lines IRE/CTVM19 and IRE/CTVM20 had modal chromosome numbers 48, 23 and 48, respectively. Also, the Ornithodoros moubata cell line OME/CTVM22 had the modal chromosome number 33 instead of 2n = 20 chromosomes for Ornithodoros spp. ticks. All studied tick cell lines had a larger genome size in comparison to the genomes of the parental ticks. Thus, highly passaged tick cell lines can be used for research purposes, but possible differences in encoded genetic information and downstream cellular processes, between different cell populations, should be taken into account. Nature Publishing Group UK 2020-08-10 /pmc/articles/PMC7417564/ /pubmed/32778731 http://dx.doi.org/10.1038/s41598-020-70330-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kotsarenko, Kateryna Vechtova, Pavlina Lieskovska, Jaroslava Füssy, Zoltán Cabral-de-Mello, Diogo C. Rego, Ryan O. M. Alberdi, Pilar Collins, Marisol Bell-Sakyi, Lesley Sterba, Jan Grubhoffer, Libor Karyotype changes in long-term cultured tick cell lines |
title | Karyotype changes in long-term cultured tick cell lines |
title_full | Karyotype changes in long-term cultured tick cell lines |
title_fullStr | Karyotype changes in long-term cultured tick cell lines |
title_full_unstemmed | Karyotype changes in long-term cultured tick cell lines |
title_short | Karyotype changes in long-term cultured tick cell lines |
title_sort | karyotype changes in long-term cultured tick cell lines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417564/ https://www.ncbi.nlm.nih.gov/pubmed/32778731 http://dx.doi.org/10.1038/s41598-020-70330-5 |
work_keys_str_mv | AT kotsarenkokateryna karyotypechangesinlongtermculturedtickcelllines AT vechtovapavlina karyotypechangesinlongtermculturedtickcelllines AT lieskovskajaroslava karyotypechangesinlongtermculturedtickcelllines AT fussyzoltan karyotypechangesinlongtermculturedtickcelllines AT cabraldemellodiogoc karyotypechangesinlongtermculturedtickcelllines AT regoryanom karyotypechangesinlongtermculturedtickcelllines AT alberdipilar karyotypechangesinlongtermculturedtickcelllines AT collinsmarisol karyotypechangesinlongtermculturedtickcelllines AT bellsakyilesley karyotypechangesinlongtermculturedtickcelllines AT sterbajan karyotypechangesinlongtermculturedtickcelllines AT grubhofferlibor karyotypechangesinlongtermculturedtickcelllines |