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Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces

Spread of pathogenic microbes and antibiotic-resistant bacteria in health-care settings and public spaces is a serious public health challenge. Materials that prevent solid surface colonization or impede touch-transfer of viable microbes could provide means to decrease pathogen transfer from high-to...

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Autores principales: Rosenberg, M., Visnapuu, M., Vija, H., Kisand, V., Kasemets, K., Kahru, A., Ivask, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417576/
https://www.ncbi.nlm.nih.gov/pubmed/32778787
http://dx.doi.org/10.1038/s41598-020-70169-w
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author Rosenberg, M.
Visnapuu, M.
Vija, H.
Kisand, V.
Kasemets, K.
Kahru, A.
Ivask, A.
author_facet Rosenberg, M.
Visnapuu, M.
Vija, H.
Kisand, V.
Kasemets, K.
Kahru, A.
Ivask, A.
author_sort Rosenberg, M.
collection PubMed
description Spread of pathogenic microbes and antibiotic-resistant bacteria in health-care settings and public spaces is a serious public health challenge. Materials that prevent solid surface colonization or impede touch-transfer of viable microbes could provide means to decrease pathogen transfer from high-touch surfaces in critical applications. ZnO and Ag nanoparticles have shown great potential in antimicrobial applications. Less is known about nano-enabled surfaces. Here we demonstrate that surfaces coated with nano-ZnO or nano-ZnO/Ag composites are not cytotoxic to human keratinocytes and possess species-selective medium-dependent antibiofilm activity against Escherichia coli, Staphylococcus aureus and Candida albicans. Colonization of nano-ZnO and nano-ZnO/Ag surfaces by E. coli and S. aureus was decreased in static oligotrophic conditions (no planktonic growth). Moderate to no effect was observed for bacterial biofilms in growth medium (supporting exponential growth). Inversely, nano-ZnO surfaces enhanced biofilm formation by C. albicans in oligotrophic conditions. However, enhanced C. albicans biofilm formation on nano-ZnO surfaces was effectively counteracted by the addition of Ag. Possible selective enhancement of biofilm formation by the yeast C. albicans on Zn-enabled surfaces should be taken into account in antimicrobial surface development. Our results also indicated the importance of the use of application-appropriate test conditions and exposure medium in antimicrobial surface testing.
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spelling pubmed-74175762020-08-11 Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces Rosenberg, M. Visnapuu, M. Vija, H. Kisand, V. Kasemets, K. Kahru, A. Ivask, A. Sci Rep Article Spread of pathogenic microbes and antibiotic-resistant bacteria in health-care settings and public spaces is a serious public health challenge. Materials that prevent solid surface colonization or impede touch-transfer of viable microbes could provide means to decrease pathogen transfer from high-touch surfaces in critical applications. ZnO and Ag nanoparticles have shown great potential in antimicrobial applications. Less is known about nano-enabled surfaces. Here we demonstrate that surfaces coated with nano-ZnO or nano-ZnO/Ag composites are not cytotoxic to human keratinocytes and possess species-selective medium-dependent antibiofilm activity against Escherichia coli, Staphylococcus aureus and Candida albicans. Colonization of nano-ZnO and nano-ZnO/Ag surfaces by E. coli and S. aureus was decreased in static oligotrophic conditions (no planktonic growth). Moderate to no effect was observed for bacterial biofilms in growth medium (supporting exponential growth). Inversely, nano-ZnO surfaces enhanced biofilm formation by C. albicans in oligotrophic conditions. However, enhanced C. albicans biofilm formation on nano-ZnO surfaces was effectively counteracted by the addition of Ag. Possible selective enhancement of biofilm formation by the yeast C. albicans on Zn-enabled surfaces should be taken into account in antimicrobial surface development. Our results also indicated the importance of the use of application-appropriate test conditions and exposure medium in antimicrobial surface testing. Nature Publishing Group UK 2020-08-10 /pmc/articles/PMC7417576/ /pubmed/32778787 http://dx.doi.org/10.1038/s41598-020-70169-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Rosenberg, M.
Visnapuu, M.
Vija, H.
Kisand, V.
Kasemets, K.
Kahru, A.
Ivask, A.
Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces
title Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces
title_full Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces
title_fullStr Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces
title_full_unstemmed Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces
title_short Selective antibiofilm properties and biocompatibility of nano-ZnO and nano-ZnO/Ag coated surfaces
title_sort selective antibiofilm properties and biocompatibility of nano-zno and nano-zno/ag coated surfaces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417576/
https://www.ncbi.nlm.nih.gov/pubmed/32778787
http://dx.doi.org/10.1038/s41598-020-70169-w
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