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Cerium Oxide-Decorated γ-Fe(2)O(3) Nanoparticles: Design, Synthesis and in vivo Effects on Parameters of Oxidative Stress
Magnetic γ-Fe(2)O(3)/CeO(x) nanoparticles were obtained by basic coprecipitation/oxidation of iron chlorides with hydrogen peroxide, followed by precipitation of Ce(NO(3))(3) with ammonia. The appearance of CeO(x) on the magnetic particle surface was confirmed by X-ray photoelectron spectroscopy (XP...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417791/ https://www.ncbi.nlm.nih.gov/pubmed/32850680 http://dx.doi.org/10.3389/fchem.2020.00682 |
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author | Moskvin, Maksym Marková, Irena Malínská, Hana Miklánková, Denisa Hüttl, Martina Oliyarnyk, Olena Pop-Georgievski, Ognen Zhigunov, Alexander Petrovský, Eduard Horák, Daniel |
author_facet | Moskvin, Maksym Marková, Irena Malínská, Hana Miklánková, Denisa Hüttl, Martina Oliyarnyk, Olena Pop-Georgievski, Ognen Zhigunov, Alexander Petrovský, Eduard Horák, Daniel |
author_sort | Moskvin, Maksym |
collection | PubMed |
description | Magnetic γ-Fe(2)O(3)/CeO(x) nanoparticles were obtained by basic coprecipitation/oxidation of iron chlorides with hydrogen peroxide, followed by precipitation of Ce(NO(3))(3) with ammonia. The appearance of CeO(x) on the magnetic particle surface was confirmed by X-ray photoelectron spectroscopy (XPS), powder X-ray diffraction (XRD), and elemental analysis; a magnetometer was used to measure the magnetic properties of γ-Fe(2)O(3)/CeO(x). The relatively high saturation magnetization of the particles (41.1 A·m(2)/kg) enabled magnetic separation. The surface of γ-Fe(2)O(3)/CeO(x) particles was functionalized with PEG-neridronate of two different molecular weights to ensure colloidal stability and biocompatibility. The ability of the particles to affect oxidative stress in hereditary hypertriglyceridemic (HHTg) rats was tested by biological assay of the liver, kidney cortex, and brain tissues. An improvement was observed in both enzymatic [superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)] and non-enzymatic (reduced (GSH) and oxidized (GSSG) glutathione) levels of antioxidant defense and lipid peroxidation parameters [4-hydroxynonenal (4-HNE) and malondialdehyde (MDA)]. The results corresponded with chemical determination of antioxidant activity based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, proving that in the animal model γ-Fe(2)O(3)/CeO(x)@PEG(2,000) nanoparticles effectively scavenged radicals due to the presence of cerium oxide, in turn decreasing oxidative stress. These particles may therefore have the potential to reduce disorders associated with oxidative stress and inflammation. |
format | Online Article Text |
id | pubmed-7417791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74177912020-08-25 Cerium Oxide-Decorated γ-Fe(2)O(3) Nanoparticles: Design, Synthesis and in vivo Effects on Parameters of Oxidative Stress Moskvin, Maksym Marková, Irena Malínská, Hana Miklánková, Denisa Hüttl, Martina Oliyarnyk, Olena Pop-Georgievski, Ognen Zhigunov, Alexander Petrovský, Eduard Horák, Daniel Front Chem Chemistry Magnetic γ-Fe(2)O(3)/CeO(x) nanoparticles were obtained by basic coprecipitation/oxidation of iron chlorides with hydrogen peroxide, followed by precipitation of Ce(NO(3))(3) with ammonia. The appearance of CeO(x) on the magnetic particle surface was confirmed by X-ray photoelectron spectroscopy (XPS), powder X-ray diffraction (XRD), and elemental analysis; a magnetometer was used to measure the magnetic properties of γ-Fe(2)O(3)/CeO(x). The relatively high saturation magnetization of the particles (41.1 A·m(2)/kg) enabled magnetic separation. The surface of γ-Fe(2)O(3)/CeO(x) particles was functionalized with PEG-neridronate of two different molecular weights to ensure colloidal stability and biocompatibility. The ability of the particles to affect oxidative stress in hereditary hypertriglyceridemic (HHTg) rats was tested by biological assay of the liver, kidney cortex, and brain tissues. An improvement was observed in both enzymatic [superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)] and non-enzymatic (reduced (GSH) and oxidized (GSSG) glutathione) levels of antioxidant defense and lipid peroxidation parameters [4-hydroxynonenal (4-HNE) and malondialdehyde (MDA)]. The results corresponded with chemical determination of antioxidant activity based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, proving that in the animal model γ-Fe(2)O(3)/CeO(x)@PEG(2,000) nanoparticles effectively scavenged radicals due to the presence of cerium oxide, in turn decreasing oxidative stress. These particles may therefore have the potential to reduce disorders associated with oxidative stress and inflammation. Frontiers Media S.A. 2020-08-04 /pmc/articles/PMC7417791/ /pubmed/32850680 http://dx.doi.org/10.3389/fchem.2020.00682 Text en Copyright © 2020 Moskvin, Marková, Malínská, Miklánková, Hüttl, Oliyarnyk, Pop-Georgievski, Zhigunov, Petrovský and Horák. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Moskvin, Maksym Marková, Irena Malínská, Hana Miklánková, Denisa Hüttl, Martina Oliyarnyk, Olena Pop-Georgievski, Ognen Zhigunov, Alexander Petrovský, Eduard Horák, Daniel Cerium Oxide-Decorated γ-Fe(2)O(3) Nanoparticles: Design, Synthesis and in vivo Effects on Parameters of Oxidative Stress |
title | Cerium Oxide-Decorated γ-Fe(2)O(3) Nanoparticles: Design, Synthesis and in vivo Effects on Parameters of Oxidative Stress |
title_full | Cerium Oxide-Decorated γ-Fe(2)O(3) Nanoparticles: Design, Synthesis and in vivo Effects on Parameters of Oxidative Stress |
title_fullStr | Cerium Oxide-Decorated γ-Fe(2)O(3) Nanoparticles: Design, Synthesis and in vivo Effects on Parameters of Oxidative Stress |
title_full_unstemmed | Cerium Oxide-Decorated γ-Fe(2)O(3) Nanoparticles: Design, Synthesis and in vivo Effects on Parameters of Oxidative Stress |
title_short | Cerium Oxide-Decorated γ-Fe(2)O(3) Nanoparticles: Design, Synthesis and in vivo Effects on Parameters of Oxidative Stress |
title_sort | cerium oxide-decorated γ-fe(2)o(3) nanoparticles: design, synthesis and in vivo effects on parameters of oxidative stress |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417791/ https://www.ncbi.nlm.nih.gov/pubmed/32850680 http://dx.doi.org/10.3389/fchem.2020.00682 |
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