Cargando…
Proteome and lysine acetylome analysis reveals insights into the molecular mechanism of seed germination in wheat
Seed germination is the first stage in wheat growth and development, directly affecting grain yield and quality. As an important post-translation modification, lysine acetylation participates in diverse biological functions. However, little is known regarding the quantitative acetylproteome characte...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7418024/ https://www.ncbi.nlm.nih.gov/pubmed/32778714 http://dx.doi.org/10.1038/s41598-020-70230-8 |
_version_ | 1783569617673781248 |
---|---|
author | Guo, Weiwei Han, Liping Li, Ximei Wang, Huifang Mu, Ping Lin, Qi Liu, Qingchang Zhang, Yumei |
author_facet | Guo, Weiwei Han, Liping Li, Ximei Wang, Huifang Mu, Ping Lin, Qi Liu, Qingchang Zhang, Yumei |
author_sort | Guo, Weiwei |
collection | PubMed |
description | Seed germination is the first stage in wheat growth and development, directly affecting grain yield and quality. As an important post-translation modification, lysine acetylation participates in diverse biological functions. However, little is known regarding the quantitative acetylproteome characterization during wheat seed germination. In this study, we generated the first comparative proteomes and lysine acetylomes during wheat seed germination. In total, 5,639 proteins and 1,301 acetylated sites on 722 proteins were identified at 0, 12 and 24 h after imbibitions. Several particularly preferred amino acids were found near acetylation sites, including K(ac)S, K(ac)T, K(ac)K, K(ac)R, K(ac)H, K(ac)F, K(ac)N, K(ac)*E, FK(ac) and K(ac)*D, in the embryos during seed germination. Among them, K(ac)H, K(ac)F, FK(ac) and K(ac)K were conserved in wheat. Biosynthetic process, transcriptional regulation, ribosome and proteasome pathway related proteins were significantly enriched in both differentially expressed proteins and differentially acetylated proteins through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis. We also revealed that histone acetylation was differentially involved in epigenetic regulation during seed germination. Meanwhile, abscisic acid and stress related proteins were found with acetylation changes. In addition, we focused on 8 enzymes involved in carbohydrate metabolism, and found they were differentially acetylated during seed germination. Finally, a putative metabolic pathway was proposed to dissect the roles of protein acetylation during wheat seed germination. These results not only demonstrate that lysine acetylation may play key roles in seed germination of wheat but also reveal insights into the molecular mechanism of seed germination in this crop. |
format | Online Article Text |
id | pubmed-7418024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-74180242020-08-13 Proteome and lysine acetylome analysis reveals insights into the molecular mechanism of seed germination in wheat Guo, Weiwei Han, Liping Li, Ximei Wang, Huifang Mu, Ping Lin, Qi Liu, Qingchang Zhang, Yumei Sci Rep Article Seed germination is the first stage in wheat growth and development, directly affecting grain yield and quality. As an important post-translation modification, lysine acetylation participates in diverse biological functions. However, little is known regarding the quantitative acetylproteome characterization during wheat seed germination. In this study, we generated the first comparative proteomes and lysine acetylomes during wheat seed germination. In total, 5,639 proteins and 1,301 acetylated sites on 722 proteins were identified at 0, 12 and 24 h after imbibitions. Several particularly preferred amino acids were found near acetylation sites, including K(ac)S, K(ac)T, K(ac)K, K(ac)R, K(ac)H, K(ac)F, K(ac)N, K(ac)*E, FK(ac) and K(ac)*D, in the embryos during seed germination. Among them, K(ac)H, K(ac)F, FK(ac) and K(ac)K were conserved in wheat. Biosynthetic process, transcriptional regulation, ribosome and proteasome pathway related proteins were significantly enriched in both differentially expressed proteins and differentially acetylated proteins through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis. We also revealed that histone acetylation was differentially involved in epigenetic regulation during seed germination. Meanwhile, abscisic acid and stress related proteins were found with acetylation changes. In addition, we focused on 8 enzymes involved in carbohydrate metabolism, and found they were differentially acetylated during seed germination. Finally, a putative metabolic pathway was proposed to dissect the roles of protein acetylation during wheat seed germination. These results not only demonstrate that lysine acetylation may play key roles in seed germination of wheat but also reveal insights into the molecular mechanism of seed germination in this crop. Nature Publishing Group UK 2020-08-10 /pmc/articles/PMC7418024/ /pubmed/32778714 http://dx.doi.org/10.1038/s41598-020-70230-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Guo, Weiwei Han, Liping Li, Ximei Wang, Huifang Mu, Ping Lin, Qi Liu, Qingchang Zhang, Yumei Proteome and lysine acetylome analysis reveals insights into the molecular mechanism of seed germination in wheat |
title | Proteome and lysine acetylome analysis reveals insights into the molecular mechanism of seed germination in wheat |
title_full | Proteome and lysine acetylome analysis reveals insights into the molecular mechanism of seed germination in wheat |
title_fullStr | Proteome and lysine acetylome analysis reveals insights into the molecular mechanism of seed germination in wheat |
title_full_unstemmed | Proteome and lysine acetylome analysis reveals insights into the molecular mechanism of seed germination in wheat |
title_short | Proteome and lysine acetylome analysis reveals insights into the molecular mechanism of seed germination in wheat |
title_sort | proteome and lysine acetylome analysis reveals insights into the molecular mechanism of seed germination in wheat |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7418024/ https://www.ncbi.nlm.nih.gov/pubmed/32778714 http://dx.doi.org/10.1038/s41598-020-70230-8 |
work_keys_str_mv | AT guoweiwei proteomeandlysineacetylomeanalysisrevealsinsightsintothemolecularmechanismofseedgerminationinwheat AT hanliping proteomeandlysineacetylomeanalysisrevealsinsightsintothemolecularmechanismofseedgerminationinwheat AT liximei proteomeandlysineacetylomeanalysisrevealsinsightsintothemolecularmechanismofseedgerminationinwheat AT wanghuifang proteomeandlysineacetylomeanalysisrevealsinsightsintothemolecularmechanismofseedgerminationinwheat AT muping proteomeandlysineacetylomeanalysisrevealsinsightsintothemolecularmechanismofseedgerminationinwheat AT linqi proteomeandlysineacetylomeanalysisrevealsinsightsintothemolecularmechanismofseedgerminationinwheat AT liuqingchang proteomeandlysineacetylomeanalysisrevealsinsightsintothemolecularmechanismofseedgerminationinwheat AT zhangyumei proteomeandlysineacetylomeanalysisrevealsinsightsintothemolecularmechanismofseedgerminationinwheat |