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Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells

OBJECTIVE: Studies have demonstrated that tetrandrine reverses multidrug resistance (MDR) in animal models or cell lines derived from multiple cancer types. We examined the potential MDR reversal activity of tetrandrine in a multidrug-resistant variant of a human laryngeal cancer Hep-2 cell line and...

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Autores principales: Li, Yachun, Li, Dongjie, Wang, Ping, Zhu, Wei, Yin, Wanzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7418264/
https://www.ncbi.nlm.nih.gov/pubmed/32776811
http://dx.doi.org/10.1177/0300060520944706
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author Li, Yachun
Li, Dongjie
Wang, Ping
Zhu, Wei
Yin, Wanzhong
author_facet Li, Yachun
Li, Dongjie
Wang, Ping
Zhu, Wei
Yin, Wanzhong
author_sort Li, Yachun
collection PubMed
description OBJECTIVE: Studies have demonstrated that tetrandrine reverses multidrug resistance (MDR) in animal models or cell lines derived from multiple cancer types. We examined the potential MDR reversal activity of tetrandrine in a multidrug-resistant variant of a human laryngeal cancer Hep-2 cell line and explored potential mechanisms involved. METHODS: We developed the multidrug-resistant variant cell line (Hep-2/v) by exposing Hep-2 cells to stepwise increasing concentrations of vincristine (VCR). After Hep-2 or Hep-2/v cells were treated with tetrandrine (2.52 µg/mL), MDR was measured by MTT assay, rhodamine 123 retention was measured by flow cytometry, and mRNA and protein expression of multidrug resistance 1 (MDR1), regulator of G-protein signaling 10 (RGS10), high-temperature requirement protein A1 (HTRA1), and nuclear protein 1 (NUPR1) were detected by real-time reverse transcription-PCR and western blotting, respectively. RESULTS: Tetrandrine significantly lowered the half-maximal inhibitory concentration (IC(50)) of VCR in Hep-2/v cells, resulting in a 2.22-fold reversal of MDR. Treatment with tetrandrine increased rhodamine 123 retention, downregulated the mRNA and protein expression of MDR1 and RGS10, and upregulated expression of HTRA1 in Hep-2/v cells. CONCLUSION: We showed that tetrandrine exerts anti-MDR activity in Hep-2/v cells, possibly by inhibiting MDR1 overexpression-mediated drug efflux and by altering expression of HTRA1 and RGS10.
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spelling pubmed-74182642020-08-24 Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells Li, Yachun Li, Dongjie Wang, Ping Zhu, Wei Yin, Wanzhong J Int Med Res Pre-Clinical Research Report OBJECTIVE: Studies have demonstrated that tetrandrine reverses multidrug resistance (MDR) in animal models or cell lines derived from multiple cancer types. We examined the potential MDR reversal activity of tetrandrine in a multidrug-resistant variant of a human laryngeal cancer Hep-2 cell line and explored potential mechanisms involved. METHODS: We developed the multidrug-resistant variant cell line (Hep-2/v) by exposing Hep-2 cells to stepwise increasing concentrations of vincristine (VCR). After Hep-2 or Hep-2/v cells were treated with tetrandrine (2.52 µg/mL), MDR was measured by MTT assay, rhodamine 123 retention was measured by flow cytometry, and mRNA and protein expression of multidrug resistance 1 (MDR1), regulator of G-protein signaling 10 (RGS10), high-temperature requirement protein A1 (HTRA1), and nuclear protein 1 (NUPR1) were detected by real-time reverse transcription-PCR and western blotting, respectively. RESULTS: Tetrandrine significantly lowered the half-maximal inhibitory concentration (IC(50)) of VCR in Hep-2/v cells, resulting in a 2.22-fold reversal of MDR. Treatment with tetrandrine increased rhodamine 123 retention, downregulated the mRNA and protein expression of MDR1 and RGS10, and upregulated expression of HTRA1 in Hep-2/v cells. CONCLUSION: We showed that tetrandrine exerts anti-MDR activity in Hep-2/v cells, possibly by inhibiting MDR1 overexpression-mediated drug efflux and by altering expression of HTRA1 and RGS10. SAGE Publications 2020-08-10 /pmc/articles/PMC7418264/ /pubmed/32776811 http://dx.doi.org/10.1177/0300060520944706 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Pre-Clinical Research Report
Li, Yachun
Li, Dongjie
Wang, Ping
Zhu, Wei
Yin, Wanzhong
Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells
title Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells
title_full Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells
title_fullStr Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells
title_full_unstemmed Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells
title_short Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells
title_sort tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells
topic Pre-Clinical Research Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7418264/
https://www.ncbi.nlm.nih.gov/pubmed/32776811
http://dx.doi.org/10.1177/0300060520944706
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