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Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells
OBJECTIVE: Studies have demonstrated that tetrandrine reverses multidrug resistance (MDR) in animal models or cell lines derived from multiple cancer types. We examined the potential MDR reversal activity of tetrandrine in a multidrug-resistant variant of a human laryngeal cancer Hep-2 cell line and...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7418264/ https://www.ncbi.nlm.nih.gov/pubmed/32776811 http://dx.doi.org/10.1177/0300060520944706 |
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author | Li, Yachun Li, Dongjie Wang, Ping Zhu, Wei Yin, Wanzhong |
author_facet | Li, Yachun Li, Dongjie Wang, Ping Zhu, Wei Yin, Wanzhong |
author_sort | Li, Yachun |
collection | PubMed |
description | OBJECTIVE: Studies have demonstrated that tetrandrine reverses multidrug resistance (MDR) in animal models or cell lines derived from multiple cancer types. We examined the potential MDR reversal activity of tetrandrine in a multidrug-resistant variant of a human laryngeal cancer Hep-2 cell line and explored potential mechanisms involved. METHODS: We developed the multidrug-resistant variant cell line (Hep-2/v) by exposing Hep-2 cells to stepwise increasing concentrations of vincristine (VCR). After Hep-2 or Hep-2/v cells were treated with tetrandrine (2.52 µg/mL), MDR was measured by MTT assay, rhodamine 123 retention was measured by flow cytometry, and mRNA and protein expression of multidrug resistance 1 (MDR1), regulator of G-protein signaling 10 (RGS10), high-temperature requirement protein A1 (HTRA1), and nuclear protein 1 (NUPR1) were detected by real-time reverse transcription-PCR and western blotting, respectively. RESULTS: Tetrandrine significantly lowered the half-maximal inhibitory concentration (IC(50)) of VCR in Hep-2/v cells, resulting in a 2.22-fold reversal of MDR. Treatment with tetrandrine increased rhodamine 123 retention, downregulated the mRNA and protein expression of MDR1 and RGS10, and upregulated expression of HTRA1 in Hep-2/v cells. CONCLUSION: We showed that tetrandrine exerts anti-MDR activity in Hep-2/v cells, possibly by inhibiting MDR1 overexpression-mediated drug efflux and by altering expression of HTRA1 and RGS10. |
format | Online Article Text |
id | pubmed-7418264 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-74182642020-08-24 Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells Li, Yachun Li, Dongjie Wang, Ping Zhu, Wei Yin, Wanzhong J Int Med Res Pre-Clinical Research Report OBJECTIVE: Studies have demonstrated that tetrandrine reverses multidrug resistance (MDR) in animal models or cell lines derived from multiple cancer types. We examined the potential MDR reversal activity of tetrandrine in a multidrug-resistant variant of a human laryngeal cancer Hep-2 cell line and explored potential mechanisms involved. METHODS: We developed the multidrug-resistant variant cell line (Hep-2/v) by exposing Hep-2 cells to stepwise increasing concentrations of vincristine (VCR). After Hep-2 or Hep-2/v cells were treated with tetrandrine (2.52 µg/mL), MDR was measured by MTT assay, rhodamine 123 retention was measured by flow cytometry, and mRNA and protein expression of multidrug resistance 1 (MDR1), regulator of G-protein signaling 10 (RGS10), high-temperature requirement protein A1 (HTRA1), and nuclear protein 1 (NUPR1) were detected by real-time reverse transcription-PCR and western blotting, respectively. RESULTS: Tetrandrine significantly lowered the half-maximal inhibitory concentration (IC(50)) of VCR in Hep-2/v cells, resulting in a 2.22-fold reversal of MDR. Treatment with tetrandrine increased rhodamine 123 retention, downregulated the mRNA and protein expression of MDR1 and RGS10, and upregulated expression of HTRA1 in Hep-2/v cells. CONCLUSION: We showed that tetrandrine exerts anti-MDR activity in Hep-2/v cells, possibly by inhibiting MDR1 overexpression-mediated drug efflux and by altering expression of HTRA1 and RGS10. SAGE Publications 2020-08-10 /pmc/articles/PMC7418264/ /pubmed/32776811 http://dx.doi.org/10.1177/0300060520944706 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Pre-Clinical Research Report Li, Yachun Li, Dongjie Wang, Ping Zhu, Wei Yin, Wanzhong Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells |
title | Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells |
title_full | Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells |
title_fullStr | Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells |
title_full_unstemmed | Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells |
title_short | Tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells |
title_sort | tetrandrine partially reverses multidrug resistance of human laryngeal cancer cells |
topic | Pre-Clinical Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7418264/ https://www.ncbi.nlm.nih.gov/pubmed/32776811 http://dx.doi.org/10.1177/0300060520944706 |
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