Cargando…

Circular RNA circTNPO3 Regulates Paclitaxel Resistance of Ovarian Cancer Cells by miR-1299/NEK2 Signaling Pathway

Circular RNAs (circRNAs) were recently reported to be involved in the pathogenesis of ovarian cancer (OC); however, the molecular mechanisms of circRNAs in tumor progression and paclitaxel (PTX) resistance of OC remain largely undetermined. Here, we focused on circTNPO3 (hsa_circ_0001741), which is...

Descripción completa

Detalles Bibliográficos
Autores principales: Xia, Bing, Zhao, Zitong, Wu, Yinnayuan, Wang, Ying, Zhao, Yan, Wang, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7419276/
https://www.ncbi.nlm.nih.gov/pubmed/32791450
http://dx.doi.org/10.1016/j.omtn.2020.06.002
_version_ 1783569849094504448
author Xia, Bing
Zhao, Zitong
Wu, Yinnayuan
Wang, Ying
Zhao, Yan
Wang, Jing
author_facet Xia, Bing
Zhao, Zitong
Wu, Yinnayuan
Wang, Ying
Zhao, Yan
Wang, Jing
author_sort Xia, Bing
collection PubMed
description Circular RNAs (circRNAs) were recently reported to be involved in the pathogenesis of ovarian cancer (OC); however, the molecular mechanisms of circRNAs in tumor progression and paclitaxel (PTX) resistance of OC remain largely undetermined. Here, we focused on circTNPO3 (hsa_circ_0001741), which is located on chromosome 7 (chr7): 128655032–128658211 and derived from TNPO3 gene, and thus we termed as circTNPO3. By microarray and qRT-PCR we identified circTNPO3 to be dramatically high expressed in OC samples and correlated with PTX resistance. Functionally, knockdown of circTNPO3 enhanced cell sensitivity to PTX via promoting PTX-induced apoptosis in vitro and in vivo. In mechanism, circTNPO3 acted as a sponge for microRNA-1299 (miR-1299), and NEK2 (NIMA-related kinase 2) was revealed to be target gene of miR-1299. Subsequently, functional assays illustrated that the oncogenic effects of circTNPO3 were attributed to the regulation of miR-1299/NEK2 axis. In conclusion, circTNPO3 contributed to PTX resistance of OC cells at least partly through upregulating NEK2 expression by sponging miR-1299. circTNPO3/miR-1299/NEK2 signaling pathway might play vital roles in the tumorigenesis and chemoresistance of OC.
format Online
Article
Text
id pubmed-7419276
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher American Society of Gene & Cell Therapy
record_format MEDLINE/PubMed
spelling pubmed-74192762020-08-14 Circular RNA circTNPO3 Regulates Paclitaxel Resistance of Ovarian Cancer Cells by miR-1299/NEK2 Signaling Pathway Xia, Bing Zhao, Zitong Wu, Yinnayuan Wang, Ying Zhao, Yan Wang, Jing Mol Ther Nucleic Acids Article Circular RNAs (circRNAs) were recently reported to be involved in the pathogenesis of ovarian cancer (OC); however, the molecular mechanisms of circRNAs in tumor progression and paclitaxel (PTX) resistance of OC remain largely undetermined. Here, we focused on circTNPO3 (hsa_circ_0001741), which is located on chromosome 7 (chr7): 128655032–128658211 and derived from TNPO3 gene, and thus we termed as circTNPO3. By microarray and qRT-PCR we identified circTNPO3 to be dramatically high expressed in OC samples and correlated with PTX resistance. Functionally, knockdown of circTNPO3 enhanced cell sensitivity to PTX via promoting PTX-induced apoptosis in vitro and in vivo. In mechanism, circTNPO3 acted as a sponge for microRNA-1299 (miR-1299), and NEK2 (NIMA-related kinase 2) was revealed to be target gene of miR-1299. Subsequently, functional assays illustrated that the oncogenic effects of circTNPO3 were attributed to the regulation of miR-1299/NEK2 axis. In conclusion, circTNPO3 contributed to PTX resistance of OC cells at least partly through upregulating NEK2 expression by sponging miR-1299. circTNPO3/miR-1299/NEK2 signaling pathway might play vital roles in the tumorigenesis and chemoresistance of OC. American Society of Gene & Cell Therapy 2020-06-08 /pmc/articles/PMC7419276/ /pubmed/32791450 http://dx.doi.org/10.1016/j.omtn.2020.06.002 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Xia, Bing
Zhao, Zitong
Wu, Yinnayuan
Wang, Ying
Zhao, Yan
Wang, Jing
Circular RNA circTNPO3 Regulates Paclitaxel Resistance of Ovarian Cancer Cells by miR-1299/NEK2 Signaling Pathway
title Circular RNA circTNPO3 Regulates Paclitaxel Resistance of Ovarian Cancer Cells by miR-1299/NEK2 Signaling Pathway
title_full Circular RNA circTNPO3 Regulates Paclitaxel Resistance of Ovarian Cancer Cells by miR-1299/NEK2 Signaling Pathway
title_fullStr Circular RNA circTNPO3 Regulates Paclitaxel Resistance of Ovarian Cancer Cells by miR-1299/NEK2 Signaling Pathway
title_full_unstemmed Circular RNA circTNPO3 Regulates Paclitaxel Resistance of Ovarian Cancer Cells by miR-1299/NEK2 Signaling Pathway
title_short Circular RNA circTNPO3 Regulates Paclitaxel Resistance of Ovarian Cancer Cells by miR-1299/NEK2 Signaling Pathway
title_sort circular rna circtnpo3 regulates paclitaxel resistance of ovarian cancer cells by mir-1299/nek2 signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7419276/
https://www.ncbi.nlm.nih.gov/pubmed/32791450
http://dx.doi.org/10.1016/j.omtn.2020.06.002
work_keys_str_mv AT xiabing circularrnacirctnpo3regulatespaclitaxelresistanceofovariancancercellsbymir1299nek2signalingpathway
AT zhaozitong circularrnacirctnpo3regulatespaclitaxelresistanceofovariancancercellsbymir1299nek2signalingpathway
AT wuyinnayuan circularrnacirctnpo3regulatespaclitaxelresistanceofovariancancercellsbymir1299nek2signalingpathway
AT wangying circularrnacirctnpo3regulatespaclitaxelresistanceofovariancancercellsbymir1299nek2signalingpathway
AT zhaoyan circularrnacirctnpo3regulatespaclitaxelresistanceofovariancancercellsbymir1299nek2signalingpathway
AT wangjing circularrnacirctnpo3regulatespaclitaxelresistanceofovariancancercellsbymir1299nek2signalingpathway