A high content, phenotypic ‘scar-in-a-jar’ assay for rapid quantification of collagen fibrillogenesis using disease-derived pulmonary fibroblasts

BACKGROUND: Excessive extracellular matrix (ECM) deposition is a hallmark feature in fibrosis and tissue remodelling diseases. Typically, mesenchymal cells will produce collagens under standard 2D cell culture conditions, however these do not assemble into fibrils. Existing assays for measuring ECM...

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Detalles Bibliográficos
Autores principales: Good, Robert B., Eley, Jessica D., Gower, Elaine, Butt, Genevieve, Blanchard, Andrew D., Fisher, Andrew J., Nanthakumar, Carmel B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7422573/
https://www.ncbi.nlm.nih.gov/pubmed/32903343
http://dx.doi.org/10.1186/s42490-019-0014-z

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