High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL)

High-throughput cytostatic and cell death assays are a critical component of pharmacological screens and mechanism-based interrogations into cellular biology. We developed a method for single-cell and population-level analyses using real-time kinetic labeling (abbreviated “SPARKL”) with non-toxic fl...

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Detalles Bibliográficos
Autores principales: Gelles, Jesse D., Chipuk, Jerry E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7423019/
https://www.ncbi.nlm.nih.gov/pubmed/32793892
http://dx.doi.org/10.1016/j.xpro.2020.100034
Descripción
Sumario:High-throughput cytostatic and cell death assays are a critical component of pharmacological screens and mechanism-based interrogations into cellular biology. We developed a method for single-cell and population-level analyses using real-time kinetic labeling (abbreviated “SPARKL”) with non-toxic fluorescent probes and high-content live-cell imagers. The protocols herein detail the steps, specifics, and suggested utilization of the SPARKL method within several “label-and-go” zero-handling workflows. For complete details on the use and execution of this protocol, please refer to Gelles et al. (2019).

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