High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL)

High-throughput cytostatic and cell death assays are a critical component of pharmacological screens and mechanism-based interrogations into cellular biology. We developed a method for single-cell and population-level analyses using real-time kinetic labeling (abbreviated “SPARKL”) with non-toxic fl...

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Detalles Bibliográficos
Autores principales: Gelles, Jesse D., Chipuk, Jerry E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7423019/
https://www.ncbi.nlm.nih.gov/pubmed/32793892
http://dx.doi.org/10.1016/j.xpro.2020.100034
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author Gelles, Jesse D.
Chipuk, Jerry E.
author_facet Gelles, Jesse D.
Chipuk, Jerry E.
author_sort Gelles, Jesse D.
collection PubMed
description High-throughput cytostatic and cell death assays are a critical component of pharmacological screens and mechanism-based interrogations into cellular biology. We developed a method for single-cell and population-level analyses using real-time kinetic labeling (abbreviated “SPARKL”) with non-toxic fluorescent probes and high-content live-cell imagers. The protocols herein detail the steps, specifics, and suggested utilization of the SPARKL method within several “label-and-go” zero-handling workflows. For complete details on the use and execution of this protocol, please refer to Gelles et al. (2019).
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spelling pubmed-74230192020-08-12 High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL) Gelles, Jesse D. Chipuk, Jerry E. STAR Protoc Protocol High-throughput cytostatic and cell death assays are a critical component of pharmacological screens and mechanism-based interrogations into cellular biology. We developed a method for single-cell and population-level analyses using real-time kinetic labeling (abbreviated “SPARKL”) with non-toxic fluorescent probes and high-content live-cell imagers. The protocols herein detail the steps, specifics, and suggested utilization of the SPARKL method within several “label-and-go” zero-handling workflows. For complete details on the use and execution of this protocol, please refer to Gelles et al. (2019). Elsevier 2020-06-03 /pmc/articles/PMC7423019/ /pubmed/32793892 http://dx.doi.org/10.1016/j.xpro.2020.100034 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Gelles, Jesse D.
Chipuk, Jerry E.
High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL)
title High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL)
title_full High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL)
title_fullStr High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL)
title_full_unstemmed High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL)
title_short High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL)
title_sort high-throughput cell death assays with single-cell and population-level analyses using real-time kinetic labeling (sparkl)
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7423019/
https://www.ncbi.nlm.nih.gov/pubmed/32793892
http://dx.doi.org/10.1016/j.xpro.2020.100034
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