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The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity

Quorum sensing (QS) is a recognized phenomenon that is crucial for regulating population-related behaviors in bacteria. However, the direct specific effect of QS molecules on host biology is largely understudied. In this work, we show that the QS molecule DSF (cis-11-methyl-dodecenoic acid) produced...

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Autores principales: Tran, Tuan Minh, Ma, Zhiming, Triebl, Alexander, Nath, Sangeeta, Cheng, Yingying, Gong, Ben-Qiang, Han, Xiao, Wang, Junqi, Li, Jian-Feng, Wenk, Markus R, Torta, Federico, Mayor, Satyajit, Yang, Liang, Miao, Yansong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425213/
https://www.ncbi.nlm.nih.gov/pubmed/32788227
http://dx.doi.org/10.26508/lsa.202000720
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author Tran, Tuan Minh
Ma, Zhiming
Triebl, Alexander
Nath, Sangeeta
Cheng, Yingying
Gong, Ben-Qiang
Han, Xiao
Wang, Junqi
Li, Jian-Feng
Wenk, Markus R
Torta, Federico
Mayor, Satyajit
Yang, Liang
Miao, Yansong
author_facet Tran, Tuan Minh
Ma, Zhiming
Triebl, Alexander
Nath, Sangeeta
Cheng, Yingying
Gong, Ben-Qiang
Han, Xiao
Wang, Junqi
Li, Jian-Feng
Wenk, Markus R
Torta, Federico
Mayor, Satyajit
Yang, Liang
Miao, Yansong
author_sort Tran, Tuan Minh
collection PubMed
description Quorum sensing (QS) is a recognized phenomenon that is crucial for regulating population-related behaviors in bacteria. However, the direct specific effect of QS molecules on host biology is largely understudied. In this work, we show that the QS molecule DSF (cis-11-methyl-dodecenoic acid) produced by Xanthomonas campestris pv. campestris can suppress pathogen-associated molecular pattern–triggered immunity (PTI) in Arabidopsis thaliana, mediated by flagellin-induced activation of flagellin receptor FLS2. The DSF-mediated attenuation of innate immunity results from the alteration of FLS2 nanoclusters and endocytic internalization of plasma membrane FLS2. DSF altered the lipid profile of Arabidopsis, with a particular increase in the phytosterol species, which impairs the general endocytosis pathway mediated by clathrin and FLS2 nano-clustering on the plasma membrane. The DSF effect on receptor dynamics and host immune responses could be entirely reversed by sterol removal. Together, our results highlighted the importance of sterol homeostasis to plasma membrane organization and demonstrate a novel mechanism by which pathogenic bacteria use their communicating molecule to manipulate pathogen-associated molecular pattern–triggered host immunity.
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spelling pubmed-74252132020-08-26 The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity Tran, Tuan Minh Ma, Zhiming Triebl, Alexander Nath, Sangeeta Cheng, Yingying Gong, Ben-Qiang Han, Xiao Wang, Junqi Li, Jian-Feng Wenk, Markus R Torta, Federico Mayor, Satyajit Yang, Liang Miao, Yansong Life Sci Alliance Research Articles Quorum sensing (QS) is a recognized phenomenon that is crucial for regulating population-related behaviors in bacteria. However, the direct specific effect of QS molecules on host biology is largely understudied. In this work, we show that the QS molecule DSF (cis-11-methyl-dodecenoic acid) produced by Xanthomonas campestris pv. campestris can suppress pathogen-associated molecular pattern–triggered immunity (PTI) in Arabidopsis thaliana, mediated by flagellin-induced activation of flagellin receptor FLS2. The DSF-mediated attenuation of innate immunity results from the alteration of FLS2 nanoclusters and endocytic internalization of plasma membrane FLS2. DSF altered the lipid profile of Arabidopsis, with a particular increase in the phytosterol species, which impairs the general endocytosis pathway mediated by clathrin and FLS2 nano-clustering on the plasma membrane. The DSF effect on receptor dynamics and host immune responses could be entirely reversed by sterol removal. Together, our results highlighted the importance of sterol homeostasis to plasma membrane organization and demonstrate a novel mechanism by which pathogenic bacteria use their communicating molecule to manipulate pathogen-associated molecular pattern–triggered host immunity. Life Science Alliance LLC 2020-08-11 /pmc/articles/PMC7425213/ /pubmed/32788227 http://dx.doi.org/10.26508/lsa.202000720 Text en © 2020 Tran et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Tran, Tuan Minh
Ma, Zhiming
Triebl, Alexander
Nath, Sangeeta
Cheng, Yingying
Gong, Ben-Qiang
Han, Xiao
Wang, Junqi
Li, Jian-Feng
Wenk, Markus R
Torta, Federico
Mayor, Satyajit
Yang, Liang
Miao, Yansong
The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity
title The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity
title_full The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity
title_fullStr The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity
title_full_unstemmed The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity
title_short The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity
title_sort bacterial quorum sensing signal dsf hijacks arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425213/
https://www.ncbi.nlm.nih.gov/pubmed/32788227
http://dx.doi.org/10.26508/lsa.202000720
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