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Exploiting somatic piRNAs in Bemisia tabaci enables novel gene silencing through RNA feeding
RNAi promises to reshape pest control by being nontoxic, biodegradable, and species specific. However, due to the plastic nature of RNAi, there is a significant variability in responses. In this study, we investigate small RNA pathways and processing of ingested RNAi trigger molecules in a hemiptera...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425214/ https://www.ncbi.nlm.nih.gov/pubmed/32764103 http://dx.doi.org/10.26508/lsa.202000731 |
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author | Mondal, Mosharrof Brown, Judith K Flynt, Alex |
author_facet | Mondal, Mosharrof Brown, Judith K Flynt, Alex |
author_sort | Mondal, Mosharrof |
collection | PubMed |
description | RNAi promises to reshape pest control by being nontoxic, biodegradable, and species specific. However, due to the plastic nature of RNAi, there is a significant variability in responses. In this study, we investigate small RNA pathways and processing of ingested RNAi trigger molecules in a hemipteran plant pest, the whitefly Bemisia tabaci. Unlike Drosophila, where the paradigm for insect RNAi technology was established, whitefly has abundant somatic piwi-associated RNAs (piRNAs). Long regarded as germline restricted, piRNAs are common in the soma of many invertebrates. We sought to exploit this for a novel gene silencing approach. The main principle of piRNA biogenesis is the recruitment of target RNA fragments into the pathway. As such, we designed synthetic RNAs to possess complementarity to the loci we annotated. Following feeding of these exogenous piRNA triggers knockdown as effective as conventional siRNA-only approaches was observed. These results demonstrate a new approach for RNAi technology that could be applicable to dsRNA-recalcitrant pest species and could be fundamental to realizing insecticidal RNAi against pests. |
format | Online Article Text |
id | pubmed-7425214 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-74252142020-08-26 Exploiting somatic piRNAs in Bemisia tabaci enables novel gene silencing through RNA feeding Mondal, Mosharrof Brown, Judith K Flynt, Alex Life Sci Alliance Research Articles RNAi promises to reshape pest control by being nontoxic, biodegradable, and species specific. However, due to the plastic nature of RNAi, there is a significant variability in responses. In this study, we investigate small RNA pathways and processing of ingested RNAi trigger molecules in a hemipteran plant pest, the whitefly Bemisia tabaci. Unlike Drosophila, where the paradigm for insect RNAi technology was established, whitefly has abundant somatic piwi-associated RNAs (piRNAs). Long regarded as germline restricted, piRNAs are common in the soma of many invertebrates. We sought to exploit this for a novel gene silencing approach. The main principle of piRNA biogenesis is the recruitment of target RNA fragments into the pathway. As such, we designed synthetic RNAs to possess complementarity to the loci we annotated. Following feeding of these exogenous piRNA triggers knockdown as effective as conventional siRNA-only approaches was observed. These results demonstrate a new approach for RNAi technology that could be applicable to dsRNA-recalcitrant pest species and could be fundamental to realizing insecticidal RNAi against pests. Life Science Alliance LLC 2020-08-06 /pmc/articles/PMC7425214/ /pubmed/32764103 http://dx.doi.org/10.26508/lsa.202000731 Text en © 2020 Mondal et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Mondal, Mosharrof Brown, Judith K Flynt, Alex Exploiting somatic piRNAs in Bemisia tabaci enables novel gene silencing through RNA feeding |
title | Exploiting somatic piRNAs in Bemisia tabaci enables novel gene silencing through RNA feeding |
title_full | Exploiting somatic piRNAs in Bemisia tabaci enables novel gene silencing through RNA feeding |
title_fullStr | Exploiting somatic piRNAs in Bemisia tabaci enables novel gene silencing through RNA feeding |
title_full_unstemmed | Exploiting somatic piRNAs in Bemisia tabaci enables novel gene silencing through RNA feeding |
title_short | Exploiting somatic piRNAs in Bemisia tabaci enables novel gene silencing through RNA feeding |
title_sort | exploiting somatic pirnas in bemisia tabaci enables novel gene silencing through rna feeding |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425214/ https://www.ncbi.nlm.nih.gov/pubmed/32764103 http://dx.doi.org/10.26508/lsa.202000731 |
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