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Dehydroandrographolide inhibits mastitis by activating autophagy without affecting intestinal flora

Mastitis can seriously damage the physical and mental health of lactating women. The use of antibiotics and anti-inflammatory drugs may damage the flora balance in lactating women. To alleviate mastitis in lactating women and reduce drug-induced damage to the flora, we found that dehydroandrographol...

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Autores principales: Guo, Wenjin, Liu, Juxiong, Zhang, Yufei, Ma, He, Li, Yuhang, Gong, Qian, Cao, Yu, Hu, Guiqiu, Xie, Shengnan, Fu, Shoupeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425474/
https://www.ncbi.nlm.nih.gov/pubmed/32702668
http://dx.doi.org/10.18632/aging.103312
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author Guo, Wenjin
Liu, Juxiong
Zhang, Yufei
Ma, He
Li, Yuhang
Gong, Qian
Cao, Yu
Hu, Guiqiu
Xie, Shengnan
Fu, Shoupeng
author_facet Guo, Wenjin
Liu, Juxiong
Zhang, Yufei
Ma, He
Li, Yuhang
Gong, Qian
Cao, Yu
Hu, Guiqiu
Xie, Shengnan
Fu, Shoupeng
author_sort Guo, Wenjin
collection PubMed
description Mastitis can seriously damage the physical and mental health of lactating women. The use of antibiotics and anti-inflammatory drugs may damage the flora balance in lactating women. To alleviate mastitis in lactating women and reduce drug-induced damage to the flora, we found that dehydroandrographolide (Deh) has good anti-inflammatory and bacterial balance functions. In vivo, we found that Deh significantly inhibited the expression of MPO, IL6, IL-1β, TNF-α, COX2 and iNOS and reduced pathological damage to the mammary gland. The feces in the control and Deh groups were collected and sequenced for 16S flora. The results showed that Deh did not change the primary intestinal microflora composition of the two groups. In vitro, our study showed that Deh significantly inhibited the expression of IL6, IL-1β and TNF-α in the EpH4-Ev cell line. When an AMPK inhibitor was added, the anti-inflammatory effect of Deh was blocked. To further study the anti-inflammatory mechanism of Deh, we found that Deh significantly promoted autophagy through the phosphorylation of AMPK, Beclin and ULK1. In conclusion, our study found that Deh promoted autophagy and played an anti-inflammatory role by activating the AMPK/Beclin/ULK1 signaling pathway and did not affect intestinal flora.
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spelling pubmed-74254742020-08-25 Dehydroandrographolide inhibits mastitis by activating autophagy without affecting intestinal flora Guo, Wenjin Liu, Juxiong Zhang, Yufei Ma, He Li, Yuhang Gong, Qian Cao, Yu Hu, Guiqiu Xie, Shengnan Fu, Shoupeng Aging (Albany NY) Research Paper Mastitis can seriously damage the physical and mental health of lactating women. The use of antibiotics and anti-inflammatory drugs may damage the flora balance in lactating women. To alleviate mastitis in lactating women and reduce drug-induced damage to the flora, we found that dehydroandrographolide (Deh) has good anti-inflammatory and bacterial balance functions. In vivo, we found that Deh significantly inhibited the expression of MPO, IL6, IL-1β, TNF-α, COX2 and iNOS and reduced pathological damage to the mammary gland. The feces in the control and Deh groups were collected and sequenced for 16S flora. The results showed that Deh did not change the primary intestinal microflora composition of the two groups. In vitro, our study showed that Deh significantly inhibited the expression of IL6, IL-1β and TNF-α in the EpH4-Ev cell line. When an AMPK inhibitor was added, the anti-inflammatory effect of Deh was blocked. To further study the anti-inflammatory mechanism of Deh, we found that Deh significantly promoted autophagy through the phosphorylation of AMPK, Beclin and ULK1. In conclusion, our study found that Deh promoted autophagy and played an anti-inflammatory role by activating the AMPK/Beclin/ULK1 signaling pathway and did not affect intestinal flora. Impact Journals 2020-07-23 /pmc/articles/PMC7425474/ /pubmed/32702668 http://dx.doi.org/10.18632/aging.103312 Text en Copyright © 2020 Guo et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Guo, Wenjin
Liu, Juxiong
Zhang, Yufei
Ma, He
Li, Yuhang
Gong, Qian
Cao, Yu
Hu, Guiqiu
Xie, Shengnan
Fu, Shoupeng
Dehydroandrographolide inhibits mastitis by activating autophagy without affecting intestinal flora
title Dehydroandrographolide inhibits mastitis by activating autophagy without affecting intestinal flora
title_full Dehydroandrographolide inhibits mastitis by activating autophagy without affecting intestinal flora
title_fullStr Dehydroandrographolide inhibits mastitis by activating autophagy without affecting intestinal flora
title_full_unstemmed Dehydroandrographolide inhibits mastitis by activating autophagy without affecting intestinal flora
title_short Dehydroandrographolide inhibits mastitis by activating autophagy without affecting intestinal flora
title_sort dehydroandrographolide inhibits mastitis by activating autophagy without affecting intestinal flora
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425474/
https://www.ncbi.nlm.nih.gov/pubmed/32702668
http://dx.doi.org/10.18632/aging.103312
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