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Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma

Osteosarcoma is a malignant, life-threatening tumor that affects children and adolescents. In this study, we identified high levels of calponin 3 (CNN3) protein in osteosarcoma tissues and cell lines. The receiver operating characteristic curve analysis revealed that CNN3 has diagnostic value for pa...

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Autores principales: Dai, Fei, Luo, Fei, Zhou, Rui, Zhou, Qiang, Xu, Jianzhong, Zhang, Zehua, Xiao, Jun, Song, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425500/
https://www.ncbi.nlm.nih.gov/pubmed/32667904
http://dx.doi.org/10.18632/aging.103224
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author Dai, Fei
Luo, Fei
Zhou, Rui
Zhou, Qiang
Xu, Jianzhong
Zhang, Zehua
Xiao, Jun
Song, Lei
author_facet Dai, Fei
Luo, Fei
Zhou, Rui
Zhou, Qiang
Xu, Jianzhong
Zhang, Zehua
Xiao, Jun
Song, Lei
author_sort Dai, Fei
collection PubMed
description Osteosarcoma is a malignant, life-threatening tumor that affects children and adolescents. In this study, we identified high levels of calponin 3 (CNN3) protein in osteosarcoma tissues and cell lines. The receiver operating characteristic curve analysis revealed that CNN3 has diagnostic value for patients with osteosarcoma. We also found that high CNN3 expression was associated with tumor size, tumor stage, and lymph node and distant metastases. Moreover, high levels of CNN3 mRNA were associated with a poor overall survival rate and a shorter disease-free survival period. CNN3 silencing inhibited cell proliferation, induced apoptosis and cell cycle arrest at the G1 stage, and inhibited cell migration and invasion in vitro. Furthermore, CNN3 silencing also inhibited subcutaneous tumor growth and lung metastasis in vivo. Western blotting revealed that silencing of CNN3 resulted in downregulated expression of MMP9, VEGF, and vimentin, and upregulation of E-cadherin. CNN3 silencing also resulted in downregulation of the ERK1/2 and p38 signaling pathways. In conclusion, high CNN3 expression was found to help in the diagnosis of osteosarcoma, and was found to be associated with poor prognosis in patients. Therefore, CNN3 may play an oncogenic role during the progression of osteosarcoma by activating the ERK1/2 and p38 pathways.
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spelling pubmed-74255002020-08-25 Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma Dai, Fei Luo, Fei Zhou, Rui Zhou, Qiang Xu, Jianzhong Zhang, Zehua Xiao, Jun Song, Lei Aging (Albany NY) Research Paper Osteosarcoma is a malignant, life-threatening tumor that affects children and adolescents. In this study, we identified high levels of calponin 3 (CNN3) protein in osteosarcoma tissues and cell lines. The receiver operating characteristic curve analysis revealed that CNN3 has diagnostic value for patients with osteosarcoma. We also found that high CNN3 expression was associated with tumor size, tumor stage, and lymph node and distant metastases. Moreover, high levels of CNN3 mRNA were associated with a poor overall survival rate and a shorter disease-free survival period. CNN3 silencing inhibited cell proliferation, induced apoptosis and cell cycle arrest at the G1 stage, and inhibited cell migration and invasion in vitro. Furthermore, CNN3 silencing also inhibited subcutaneous tumor growth and lung metastasis in vivo. Western blotting revealed that silencing of CNN3 resulted in downregulated expression of MMP9, VEGF, and vimentin, and upregulation of E-cadherin. CNN3 silencing also resulted in downregulation of the ERK1/2 and p38 signaling pathways. In conclusion, high CNN3 expression was found to help in the diagnosis of osteosarcoma, and was found to be associated with poor prognosis in patients. Therefore, CNN3 may play an oncogenic role during the progression of osteosarcoma by activating the ERK1/2 and p38 pathways. Impact Journals 2020-07-14 /pmc/articles/PMC7425500/ /pubmed/32667904 http://dx.doi.org/10.18632/aging.103224 Text en Copyright © 2020 Dai et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Dai, Fei
Luo, Fei
Zhou, Rui
Zhou, Qiang
Xu, Jianzhong
Zhang, Zehua
Xiao, Jun
Song, Lei
Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma
title Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma
title_full Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma
title_fullStr Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma
title_full_unstemmed Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma
title_short Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma
title_sort calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425500/
https://www.ncbi.nlm.nih.gov/pubmed/32667904
http://dx.doi.org/10.18632/aging.103224
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