Cargando…
The effects of thylakoid-rich spinach extract and aqueous extract of caraway (Carum carvi L.) in letrozole-induced polycystic ovarian syndrome rats
BACKGROUND: Polycystic ovary syndrome (PCOS) is a common endocrine disorder. The aim of the present study was to evaluate the effects of the oral administration of thylakoid-rich spinach extract and the caraway aqueous extract in letrozole-induced polycystic ovary syndrome rats. METHODS: Sixty femal...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425561/ https://www.ncbi.nlm.nih.gov/pubmed/32787839 http://dx.doi.org/10.1186/s12906-020-03044-w |
_version_ | 1783570521457164288 |
---|---|
author | Sherafatmanesh, Saeed Ekramzadeh, Maryam Tanideh, Nader Golmakani, Mohammad-Taghi Koohpeyma, Farhad |
author_facet | Sherafatmanesh, Saeed Ekramzadeh, Maryam Tanideh, Nader Golmakani, Mohammad-Taghi Koohpeyma, Farhad |
author_sort | Sherafatmanesh, Saeed |
collection | PubMed |
description | BACKGROUND: Polycystic ovary syndrome (PCOS) is a common endocrine disorder. The aim of the present study was to evaluate the effects of the oral administration of thylakoid-rich spinach extract and the caraway aqueous extract in letrozole-induced polycystic ovary syndrome rats. METHODS: Sixty female Sprague-Dawley rats were randomly divided into five groups of 12 animals each. Letrozole (1 mg/kg) was administered orally for a period of 28 days to induce PCOS. Sham and PCOS control rats received 1 mL/day of distilled water, and the three groups of PCOS rats were given 150 mg/kg of metformin, 3 g/kg of caraway, and thylakoid at a dose of 6 mg chlorophyll/gr food intake/day by oral gavage for 8 weeks. Finally, blood samples were collected and the right ovary of rats was removed, weighed, and fixed in 4% buffered formalin to determine the biochemical and stereological parameters. RESULTS: Compared to the PCOS control group, consuming metformin, thylakoid, and caraway extracts significantly improved the fasting blood sugar (FBS), tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), luteinizing hormone (LH), insulin resistance, and body weight, increased the volume of the corpus luteum, and reduced the number of atretic follicles after 8 weeks (푃< 0.05). Although caraway treatment caused a significant increase in the HDL-C (High-density lipoprotein cholesterol) level (P < 0.001), no significant change was observed in terms of HDL-C in the thylakoid and metformin groups compared to the PCOS control group. CONCLUSION: Our data showed that the consumption of thylakoid and caraway extracts for 8 weeks may have beneficial effects on the biochemical and stereological factors in PCOS-induced rats. |
format | Online Article Text |
id | pubmed-7425561 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-74255612020-08-16 The effects of thylakoid-rich spinach extract and aqueous extract of caraway (Carum carvi L.) in letrozole-induced polycystic ovarian syndrome rats Sherafatmanesh, Saeed Ekramzadeh, Maryam Tanideh, Nader Golmakani, Mohammad-Taghi Koohpeyma, Farhad BMC Complement Med Ther Research Article BACKGROUND: Polycystic ovary syndrome (PCOS) is a common endocrine disorder. The aim of the present study was to evaluate the effects of the oral administration of thylakoid-rich spinach extract and the caraway aqueous extract in letrozole-induced polycystic ovary syndrome rats. METHODS: Sixty female Sprague-Dawley rats were randomly divided into five groups of 12 animals each. Letrozole (1 mg/kg) was administered orally for a period of 28 days to induce PCOS. Sham and PCOS control rats received 1 mL/day of distilled water, and the three groups of PCOS rats were given 150 mg/kg of metformin, 3 g/kg of caraway, and thylakoid at a dose of 6 mg chlorophyll/gr food intake/day by oral gavage for 8 weeks. Finally, blood samples were collected and the right ovary of rats was removed, weighed, and fixed in 4% buffered formalin to determine the biochemical and stereological parameters. RESULTS: Compared to the PCOS control group, consuming metformin, thylakoid, and caraway extracts significantly improved the fasting blood sugar (FBS), tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), luteinizing hormone (LH), insulin resistance, and body weight, increased the volume of the corpus luteum, and reduced the number of atretic follicles after 8 weeks (푃< 0.05). Although caraway treatment caused a significant increase in the HDL-C (High-density lipoprotein cholesterol) level (P < 0.001), no significant change was observed in terms of HDL-C in the thylakoid and metformin groups compared to the PCOS control group. CONCLUSION: Our data showed that the consumption of thylakoid and caraway extracts for 8 weeks may have beneficial effects on the biochemical and stereological factors in PCOS-induced rats. BioMed Central 2020-08-12 /pmc/articles/PMC7425561/ /pubmed/32787839 http://dx.doi.org/10.1186/s12906-020-03044-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Sherafatmanesh, Saeed Ekramzadeh, Maryam Tanideh, Nader Golmakani, Mohammad-Taghi Koohpeyma, Farhad The effects of thylakoid-rich spinach extract and aqueous extract of caraway (Carum carvi L.) in letrozole-induced polycystic ovarian syndrome rats |
title | The effects of thylakoid-rich spinach extract and aqueous extract of caraway (Carum carvi L.) in letrozole-induced polycystic ovarian syndrome rats |
title_full | The effects of thylakoid-rich spinach extract and aqueous extract of caraway (Carum carvi L.) in letrozole-induced polycystic ovarian syndrome rats |
title_fullStr | The effects of thylakoid-rich spinach extract and aqueous extract of caraway (Carum carvi L.) in letrozole-induced polycystic ovarian syndrome rats |
title_full_unstemmed | The effects of thylakoid-rich spinach extract and aqueous extract of caraway (Carum carvi L.) in letrozole-induced polycystic ovarian syndrome rats |
title_short | The effects of thylakoid-rich spinach extract and aqueous extract of caraway (Carum carvi L.) in letrozole-induced polycystic ovarian syndrome rats |
title_sort | effects of thylakoid-rich spinach extract and aqueous extract of caraway (carum carvi l.) in letrozole-induced polycystic ovarian syndrome rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425561/ https://www.ncbi.nlm.nih.gov/pubmed/32787839 http://dx.doi.org/10.1186/s12906-020-03044-w |
work_keys_str_mv | AT sherafatmaneshsaeed theeffectsofthylakoidrichspinachextractandaqueousextractofcarawaycarumcarvilinletrozoleinducedpolycysticovariansyndromerats AT ekramzadehmaryam theeffectsofthylakoidrichspinachextractandaqueousextractofcarawaycarumcarvilinletrozoleinducedpolycysticovariansyndromerats AT tanidehnader theeffectsofthylakoidrichspinachextractandaqueousextractofcarawaycarumcarvilinletrozoleinducedpolycysticovariansyndromerats AT golmakanimohammadtaghi theeffectsofthylakoidrichspinachextractandaqueousextractofcarawaycarumcarvilinletrozoleinducedpolycysticovariansyndromerats AT koohpeymafarhad theeffectsofthylakoidrichspinachextractandaqueousextractofcarawaycarumcarvilinletrozoleinducedpolycysticovariansyndromerats AT sherafatmaneshsaeed effectsofthylakoidrichspinachextractandaqueousextractofcarawaycarumcarvilinletrozoleinducedpolycysticovariansyndromerats AT ekramzadehmaryam effectsofthylakoidrichspinachextractandaqueousextractofcarawaycarumcarvilinletrozoleinducedpolycysticovariansyndromerats AT tanidehnader effectsofthylakoidrichspinachextractandaqueousextractofcarawaycarumcarvilinletrozoleinducedpolycysticovariansyndromerats AT golmakanimohammadtaghi effectsofthylakoidrichspinachextractandaqueousextractofcarawaycarumcarvilinletrozoleinducedpolycysticovariansyndromerats AT koohpeymafarhad effectsofthylakoidrichspinachextractandaqueousextractofcarawaycarumcarvilinletrozoleinducedpolycysticovariansyndromerats |