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Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens

Increased intestinal permeability (IP) and inflammation are both linked with functionality of the intestinal barrier and in particular enterocytes. Currently, almost all assessment methods of the intestinal barrier function are invasive. The present study aimed to quantify selected proteins as novel...

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Autores principales: Barekatain, Reza, Howarth, Gordon S., Willson, Nicky-Lee, Cadogan, David, Wilkinson, Stuart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425878/
https://www.ncbi.nlm.nih.gov/pubmed/32790727
http://dx.doi.org/10.1371/journal.pone.0237505
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author Barekatain, Reza
Howarth, Gordon S.
Willson, Nicky-Lee
Cadogan, David
Wilkinson, Stuart
author_facet Barekatain, Reza
Howarth, Gordon S.
Willson, Nicky-Lee
Cadogan, David
Wilkinson, Stuart
author_sort Barekatain, Reza
collection PubMed
description Increased intestinal permeability (IP) and inflammation are both linked with functionality of the intestinal barrier and in particular enterocytes. Currently, almost all assessment methods of the intestinal barrier function are invasive. The present study aimed to quantify selected proteins as novel biomarkers in excreta of broiler chickens to facilitate non-invasive assessment of gut barrier function using enzyme-linked immunosorbent assays (ELISA). It was further hypothesised that probiotics as feed additives may counteract gut barrier dysfunction. A 3 × 2 factorial arrangement of treatments was used with the main factors being gut barrier dysfunction models (control, rye-based diet, and dexamethasone–DEX) with and without probiotic supplementation (a three-strain Bacillus) using 72 male Ross 308 day-old chickens. Each of the 6 experimental treatments was replicated 12 times. On d 21 of age, fluorescein isothiocyanate dextran (FITC-d) uptake into serum was examined to test IP. Fresh excreta samples were collected on d 20. The biomarkers included alpha-1 antitrypsin (A1AT), intestinal fatty acid binding protein (I-FABP), lipocalin-2 (LCN2), fibronectin (FN), intestinal alkaline phosphatase (IAP), ovotransferrin (OVT) and superoxide dismutase [Cu-Zn] (SOD1). Only DEX increased (P<0.001) FITC-d passage to the blood on d 21 of age, indicating a greater IP. The excreta concentrations of A1AT, I-FABP and SOD1 were unaltered by the experimental treatments. DEX increased (P<0.05) FN concentration in excreta compared with control birds. Conversely, inclusion of rye in the diet reduced (P<0.05) FN but increased (P<0.001) OVT in excreta. Independently, DEX decreased IAP (P<0.05) in excreta compared with control and rye-fed birds. The excreta concentration of LCN2 tended (P = 0.086) to increase in birds injected by DEX. There was no demonstrable effect of probiotic addition on any of the studied parameters. Among the tested biomarkers, FN, IAP, and LCN2 revealed promise as biomarkers of intestinal barrier function quantified by ELISA kits.
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spelling pubmed-74258782020-08-20 Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens Barekatain, Reza Howarth, Gordon S. Willson, Nicky-Lee Cadogan, David Wilkinson, Stuart PLoS One Research Article Increased intestinal permeability (IP) and inflammation are both linked with functionality of the intestinal barrier and in particular enterocytes. Currently, almost all assessment methods of the intestinal barrier function are invasive. The present study aimed to quantify selected proteins as novel biomarkers in excreta of broiler chickens to facilitate non-invasive assessment of gut barrier function using enzyme-linked immunosorbent assays (ELISA). It was further hypothesised that probiotics as feed additives may counteract gut barrier dysfunction. A 3 × 2 factorial arrangement of treatments was used with the main factors being gut barrier dysfunction models (control, rye-based diet, and dexamethasone–DEX) with and without probiotic supplementation (a three-strain Bacillus) using 72 male Ross 308 day-old chickens. Each of the 6 experimental treatments was replicated 12 times. On d 21 of age, fluorescein isothiocyanate dextran (FITC-d) uptake into serum was examined to test IP. Fresh excreta samples were collected on d 20. The biomarkers included alpha-1 antitrypsin (A1AT), intestinal fatty acid binding protein (I-FABP), lipocalin-2 (LCN2), fibronectin (FN), intestinal alkaline phosphatase (IAP), ovotransferrin (OVT) and superoxide dismutase [Cu-Zn] (SOD1). Only DEX increased (P<0.001) FITC-d passage to the blood on d 21 of age, indicating a greater IP. The excreta concentrations of A1AT, I-FABP and SOD1 were unaltered by the experimental treatments. DEX increased (P<0.05) FN concentration in excreta compared with control birds. Conversely, inclusion of rye in the diet reduced (P<0.05) FN but increased (P<0.001) OVT in excreta. Independently, DEX decreased IAP (P<0.05) in excreta compared with control and rye-fed birds. The excreta concentration of LCN2 tended (P = 0.086) to increase in birds injected by DEX. There was no demonstrable effect of probiotic addition on any of the studied parameters. Among the tested biomarkers, FN, IAP, and LCN2 revealed promise as biomarkers of intestinal barrier function quantified by ELISA kits. Public Library of Science 2020-08-13 /pmc/articles/PMC7425878/ /pubmed/32790727 http://dx.doi.org/10.1371/journal.pone.0237505 Text en © 2020 Barekatain et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Barekatain, Reza
Howarth, Gordon S.
Willson, Nicky-Lee
Cadogan, David
Wilkinson, Stuart
Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens
title Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens
title_full Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens
title_fullStr Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens
title_full_unstemmed Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens
title_short Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens
title_sort excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425878/
https://www.ncbi.nlm.nih.gov/pubmed/32790727
http://dx.doi.org/10.1371/journal.pone.0237505
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