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Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolates
To prescribe effective antibiotics to patients with bacterial infections in a timely manner and to avoid the misuse of antibiotics, a rapid antimicrobial susceptibility test (AST) is essential. However, conventional AST methods require more than 16 h to provide results; thus, we developed an electri...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426404/ https://www.ncbi.nlm.nih.gov/pubmed/32792573 http://dx.doi.org/10.1038/s41598-020-70459-3 |
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author | Lee, Kyo-Seok Lee, Sun-Mi Oh, Jeseung Park, In Ho Song, Jun Ho Han, Myeonggil Yong, Dongeun Lim, Kook Jin Shin, Jeon-Soo Yoo, Kyung-Hwa |
author_facet | Lee, Kyo-Seok Lee, Sun-Mi Oh, Jeseung Park, In Ho Song, Jun Ho Han, Myeonggil Yong, Dongeun Lim, Kook Jin Shin, Jeon-Soo Yoo, Kyung-Hwa |
author_sort | Lee, Kyo-Seok |
collection | PubMed |
description | To prescribe effective antibiotics to patients with bacterial infections in a timely manner and to avoid the misuse of antibiotics, a rapid antimicrobial susceptibility test (AST) is essential. However, conventional AST methods require more than 16 h to provide results; thus, we developed an electrical AST (e-AST) system, which provides results within 6 h. The proposed e-AST is based on an array of 60 aptamer-functionalized capacitance sensors that are comparable to currently available AST panels and a pattern-matching algorithm. The performance of the e-AST was evaluated in comparison with that of broth microdilution as the reference test for clinical strains isolated from septic patients. A total of 4,554 tests using e-AST showed a categorical agreement of 97% with a minor error of 2.2%, major error of 0.38%, and very major error of 0.38%. We expect that the proposed e-AST could potentially aid antimicrobial stewardship efforts and lead to improved patient outcomes. |
format | Online Article Text |
id | pubmed-7426404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-74264042020-08-14 Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolates Lee, Kyo-Seok Lee, Sun-Mi Oh, Jeseung Park, In Ho Song, Jun Ho Han, Myeonggil Yong, Dongeun Lim, Kook Jin Shin, Jeon-Soo Yoo, Kyung-Hwa Sci Rep Article To prescribe effective antibiotics to patients with bacterial infections in a timely manner and to avoid the misuse of antibiotics, a rapid antimicrobial susceptibility test (AST) is essential. However, conventional AST methods require more than 16 h to provide results; thus, we developed an electrical AST (e-AST) system, which provides results within 6 h. The proposed e-AST is based on an array of 60 aptamer-functionalized capacitance sensors that are comparable to currently available AST panels and a pattern-matching algorithm. The performance of the e-AST was evaluated in comparison with that of broth microdilution as the reference test for clinical strains isolated from septic patients. A total of 4,554 tests using e-AST showed a categorical agreement of 97% with a minor error of 2.2%, major error of 0.38%, and very major error of 0.38%. We expect that the proposed e-AST could potentially aid antimicrobial stewardship efforts and lead to improved patient outcomes. Nature Publishing Group UK 2020-08-13 /pmc/articles/PMC7426404/ /pubmed/32792573 http://dx.doi.org/10.1038/s41598-020-70459-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lee, Kyo-Seok Lee, Sun-Mi Oh, Jeseung Park, In Ho Song, Jun Ho Han, Myeonggil Yong, Dongeun Lim, Kook Jin Shin, Jeon-Soo Yoo, Kyung-Hwa Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolates |
title | Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolates |
title_full | Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolates |
title_fullStr | Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolates |
title_full_unstemmed | Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolates |
title_short | Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolates |
title_sort | electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolates |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426404/ https://www.ncbi.nlm.nih.gov/pubmed/32792573 http://dx.doi.org/10.1038/s41598-020-70459-3 |
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