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Approaches for Genetic Discoveries in the Skin Commensal and Pathogenic Malassezia Yeasts
Malassezia includes yeasts belong to the subphylum Ustilaginomycotina within the Basidiomycota. Malassezia yeasts are commonly found as commensals on human and animal skin. Nevertheless, Malassezia species are also associated with several skin disorders, such as dandruff/seborrheic dermatitis, atopi...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426719/ https://www.ncbi.nlm.nih.gov/pubmed/32850491 http://dx.doi.org/10.3389/fcimb.2020.00393 |
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author | Ianiri, Giuseppe Heitman, Joseph |
author_facet | Ianiri, Giuseppe Heitman, Joseph |
author_sort | Ianiri, Giuseppe |
collection | PubMed |
description | Malassezia includes yeasts belong to the subphylum Ustilaginomycotina within the Basidiomycota. Malassezia yeasts are commonly found as commensals on human and animal skin. Nevertheless, Malassezia species are also associated with several skin disorders, such as dandruff/seborrheic dermatitis, atopic eczema, pityriasis versicolor, and folliculitis. More recently, associations of Malassezia with Crohn's disease, pancreatic ductal adenocarcinoma, and cystic fibrosis pulmonary exacerbation have been reported. The increasing availability of genomic and molecular tools have played a crucial role in understanding the genetic basis of Malassezia commensalism and pathogenicity. In the present review we report genomics advances in Malassezia highlighting unique features that potentially impacted Malassezia biology and host adaptation. Furthermore, we describe the recently developed protocols for Agrobacterium tumefaciens-mediated transformation in Malassezia, and their applications for random insertional mutagenesis or targeted gene replacement strategies. |
format | Online Article Text |
id | pubmed-7426719 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74267192020-08-25 Approaches for Genetic Discoveries in the Skin Commensal and Pathogenic Malassezia Yeasts Ianiri, Giuseppe Heitman, Joseph Front Cell Infect Microbiol Cellular and Infection Microbiology Malassezia includes yeasts belong to the subphylum Ustilaginomycotina within the Basidiomycota. Malassezia yeasts are commonly found as commensals on human and animal skin. Nevertheless, Malassezia species are also associated with several skin disorders, such as dandruff/seborrheic dermatitis, atopic eczema, pityriasis versicolor, and folliculitis. More recently, associations of Malassezia with Crohn's disease, pancreatic ductal adenocarcinoma, and cystic fibrosis pulmonary exacerbation have been reported. The increasing availability of genomic and molecular tools have played a crucial role in understanding the genetic basis of Malassezia commensalism and pathogenicity. In the present review we report genomics advances in Malassezia highlighting unique features that potentially impacted Malassezia biology and host adaptation. Furthermore, we describe the recently developed protocols for Agrobacterium tumefaciens-mediated transformation in Malassezia, and their applications for random insertional mutagenesis or targeted gene replacement strategies. Frontiers Media S.A. 2020-08-07 /pmc/articles/PMC7426719/ /pubmed/32850491 http://dx.doi.org/10.3389/fcimb.2020.00393 Text en Copyright © 2020 Ianiri and Heitman. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Ianiri, Giuseppe Heitman, Joseph Approaches for Genetic Discoveries in the Skin Commensal and Pathogenic Malassezia Yeasts |
title | Approaches for Genetic Discoveries in the Skin Commensal and Pathogenic Malassezia Yeasts |
title_full | Approaches for Genetic Discoveries in the Skin Commensal and Pathogenic Malassezia Yeasts |
title_fullStr | Approaches for Genetic Discoveries in the Skin Commensal and Pathogenic Malassezia Yeasts |
title_full_unstemmed | Approaches for Genetic Discoveries in the Skin Commensal and Pathogenic Malassezia Yeasts |
title_short | Approaches for Genetic Discoveries in the Skin Commensal and Pathogenic Malassezia Yeasts |
title_sort | approaches for genetic discoveries in the skin commensal and pathogenic malassezia yeasts |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426719/ https://www.ncbi.nlm.nih.gov/pubmed/32850491 http://dx.doi.org/10.3389/fcimb.2020.00393 |
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