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Decreased Expression of CD69 on T Cells in Tuberculosis Infection Resisters

BACKGROUND: CD69 is a biomarker of T-cell activation status, but its activation status in human Mycobacterium tuberculosis (Mtb) infection remains elusive. METHODS: A set of cohorts of patients with different tuberculosis (TB) infection status including active TB patients (ATB), latent tuberculous i...

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Autores principales: Chen, Zhen-Yan, Wang, Lei, Gu, Ling, Qu, Rong, Lowrie, Douglas B., Hu, Zhidong, Sha, Wei, Fan, Xiao-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426741/
https://www.ncbi.nlm.nih.gov/pubmed/32849474
http://dx.doi.org/10.3389/fmicb.2020.01901
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author Chen, Zhen-Yan
Wang, Lei
Gu, Ling
Qu, Rong
Lowrie, Douglas B.
Hu, Zhidong
Sha, Wei
Fan, Xiao-Yong
author_facet Chen, Zhen-Yan
Wang, Lei
Gu, Ling
Qu, Rong
Lowrie, Douglas B.
Hu, Zhidong
Sha, Wei
Fan, Xiao-Yong
author_sort Chen, Zhen-Yan
collection PubMed
description BACKGROUND: CD69 is a biomarker of T-cell activation status, but its activation status in human Mycobacterium tuberculosis (Mtb) infection remains elusive. METHODS: A set of cohorts of patients with different tuberculosis (TB) infection status including active TB patients (ATB), latent tuberculous infection patients (LTBI) and close contacts (CCs) of ATB was designed, and the expression profiles of CD69 and several T-cell markers were determined on Mtb antigen-stimulated T cells by flow cytometry. RESULTS: The frequencies of CD4(+) and CD8(+) T cells were both comparable among Mtb-infected individuals including ATB and LTBI, which guaranteed the consistency of the background level. A t-Distributed Stochastic Neighbor Embedding (tSNE) analysis on a panel of six phenotypic markers showed a unique color map axis gated on T cells in the CCs group compared with ATB and LTBI populations. By further gating on cells positive for each individual marker and then overlaying those events on top of the tSNE plots, their distribution suggested that some markers were expressed differently in the CCs group. Further analysis showed that the expression levels of CD69 on both CD4(+) and CD8(+) T cells were significantly lower in the CCs group, especially in interferon-γ-responding T cells. CONCLUSION: Our findings suggest that the T-cell activation status of CD69 is associated with Mtb infection and may have the potential to distinguish LTBI from those populations who have been exposed continuously to Mtb but have not become infected.
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spelling pubmed-74267412020-08-25 Decreased Expression of CD69 on T Cells in Tuberculosis Infection Resisters Chen, Zhen-Yan Wang, Lei Gu, Ling Qu, Rong Lowrie, Douglas B. Hu, Zhidong Sha, Wei Fan, Xiao-Yong Front Microbiol Microbiology BACKGROUND: CD69 is a biomarker of T-cell activation status, but its activation status in human Mycobacterium tuberculosis (Mtb) infection remains elusive. METHODS: A set of cohorts of patients with different tuberculosis (TB) infection status including active TB patients (ATB), latent tuberculous infection patients (LTBI) and close contacts (CCs) of ATB was designed, and the expression profiles of CD69 and several T-cell markers were determined on Mtb antigen-stimulated T cells by flow cytometry. RESULTS: The frequencies of CD4(+) and CD8(+) T cells were both comparable among Mtb-infected individuals including ATB and LTBI, which guaranteed the consistency of the background level. A t-Distributed Stochastic Neighbor Embedding (tSNE) analysis on a panel of six phenotypic markers showed a unique color map axis gated on T cells in the CCs group compared with ATB and LTBI populations. By further gating on cells positive for each individual marker and then overlaying those events on top of the tSNE plots, their distribution suggested that some markers were expressed differently in the CCs group. Further analysis showed that the expression levels of CD69 on both CD4(+) and CD8(+) T cells were significantly lower in the CCs group, especially in interferon-γ-responding T cells. CONCLUSION: Our findings suggest that the T-cell activation status of CD69 is associated with Mtb infection and may have the potential to distinguish LTBI from those populations who have been exposed continuously to Mtb but have not become infected. Frontiers Media S.A. 2020-08-07 /pmc/articles/PMC7426741/ /pubmed/32849474 http://dx.doi.org/10.3389/fmicb.2020.01901 Text en Copyright © 2020 Chen, Wang, Gu, Qu, Lowrie, Hu, Sha and Fan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Chen, Zhen-Yan
Wang, Lei
Gu, Ling
Qu, Rong
Lowrie, Douglas B.
Hu, Zhidong
Sha, Wei
Fan, Xiao-Yong
Decreased Expression of CD69 on T Cells in Tuberculosis Infection Resisters
title Decreased Expression of CD69 on T Cells in Tuberculosis Infection Resisters
title_full Decreased Expression of CD69 on T Cells in Tuberculosis Infection Resisters
title_fullStr Decreased Expression of CD69 on T Cells in Tuberculosis Infection Resisters
title_full_unstemmed Decreased Expression of CD69 on T Cells in Tuberculosis Infection Resisters
title_short Decreased Expression of CD69 on T Cells in Tuberculosis Infection Resisters
title_sort decreased expression of cd69 on t cells in tuberculosis infection resisters
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426741/
https://www.ncbi.nlm.nih.gov/pubmed/32849474
http://dx.doi.org/10.3389/fmicb.2020.01901
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