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Long non-coding RNA FENDRR regulates IFNγ-induced M1 phenotype in macrophages
Macrophages play an essential role in host defense and display remarkable plasticity in switching between classically (pro-inflammatory—M1) and alternatively activated (anti-inflammatory—M2) phenotypes. The molecular mechanisms of macrophage polarization are not fully understood. Long non-coding RNA...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426844/ https://www.ncbi.nlm.nih.gov/pubmed/32792604 http://dx.doi.org/10.1038/s41598-020-70633-7 |
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author | Munteanu, Maria Cristina Huang, Chaoqun Liang, Yurong Sathiaseelan, Roshini Zeng, Xiangming Liu, Lin |
author_facet | Munteanu, Maria Cristina Huang, Chaoqun Liang, Yurong Sathiaseelan, Roshini Zeng, Xiangming Liu, Lin |
author_sort | Munteanu, Maria Cristina |
collection | PubMed |
description | Macrophages play an essential role in host defense and display remarkable plasticity in switching between classically (pro-inflammatory—M1) and alternatively activated (anti-inflammatory—M2) phenotypes. The molecular mechanisms of macrophage polarization are not fully understood. Long non-coding RNAs (lncRNAs) with a length of > 200 nucleotides have been shown to play diverse roles in biological processes. Aberrant expression of lncRNAs is associated with a variety of pathophysiological conditions such as cancer, diabetes, cardiovascular, pulmonary diseases, and tissue fibrosis. In this study, we investigated the role of lncRNA FENDRR in human and mouse macrophage polarization. Human THP-1 monocytes were activated with phorbol-12-myristate-13-acetate (PMA) and differentiated into M1 macrophages with IFNγ or M2 macrophages with IL4. Real-time PCR analysis revealed that FENDRR was expressed 80-fold higher in M1 macrophages than that in M2 macrophages. Overexpression of FENDRR in PMA-activated THP-1 cells increased the IFNγ-induced expression of M1 markers, including IL1β and TNFα at both mRNA and protein levels. Knockdown of FENDRR had an opposite effect. Similarly, FENDRR overexpression in primary mouse bone marrow-derived macrophages increased mRNA expression of M1 markers. FENDRR overexpression increased, while FENDRR knock-down decreased, the IFNγ-induced phosphorylation of STAT1 in PMA-activated THP-1 cells. Our studies suggest that FENDRR enhances IFNγ-induced M1 macrophage polarization via the STAT1 pathway. |
format | Online Article Text |
id | pubmed-7426844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-74268442020-08-14 Long non-coding RNA FENDRR regulates IFNγ-induced M1 phenotype in macrophages Munteanu, Maria Cristina Huang, Chaoqun Liang, Yurong Sathiaseelan, Roshini Zeng, Xiangming Liu, Lin Sci Rep Article Macrophages play an essential role in host defense and display remarkable plasticity in switching between classically (pro-inflammatory—M1) and alternatively activated (anti-inflammatory—M2) phenotypes. The molecular mechanisms of macrophage polarization are not fully understood. Long non-coding RNAs (lncRNAs) with a length of > 200 nucleotides have been shown to play diverse roles in biological processes. Aberrant expression of lncRNAs is associated with a variety of pathophysiological conditions such as cancer, diabetes, cardiovascular, pulmonary diseases, and tissue fibrosis. In this study, we investigated the role of lncRNA FENDRR in human and mouse macrophage polarization. Human THP-1 monocytes were activated with phorbol-12-myristate-13-acetate (PMA) and differentiated into M1 macrophages with IFNγ or M2 macrophages with IL4. Real-time PCR analysis revealed that FENDRR was expressed 80-fold higher in M1 macrophages than that in M2 macrophages. Overexpression of FENDRR in PMA-activated THP-1 cells increased the IFNγ-induced expression of M1 markers, including IL1β and TNFα at both mRNA and protein levels. Knockdown of FENDRR had an opposite effect. Similarly, FENDRR overexpression in primary mouse bone marrow-derived macrophages increased mRNA expression of M1 markers. FENDRR overexpression increased, while FENDRR knock-down decreased, the IFNγ-induced phosphorylation of STAT1 in PMA-activated THP-1 cells. Our studies suggest that FENDRR enhances IFNγ-induced M1 macrophage polarization via the STAT1 pathway. Nature Publishing Group UK 2020-08-13 /pmc/articles/PMC7426844/ /pubmed/32792604 http://dx.doi.org/10.1038/s41598-020-70633-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Munteanu, Maria Cristina Huang, Chaoqun Liang, Yurong Sathiaseelan, Roshini Zeng, Xiangming Liu, Lin Long non-coding RNA FENDRR regulates IFNγ-induced M1 phenotype in macrophages |
title | Long non-coding RNA FENDRR regulates IFNγ-induced M1 phenotype in macrophages |
title_full | Long non-coding RNA FENDRR regulates IFNγ-induced M1 phenotype in macrophages |
title_fullStr | Long non-coding RNA FENDRR regulates IFNγ-induced M1 phenotype in macrophages |
title_full_unstemmed | Long non-coding RNA FENDRR regulates IFNγ-induced M1 phenotype in macrophages |
title_short | Long non-coding RNA FENDRR regulates IFNγ-induced M1 phenotype in macrophages |
title_sort | long non-coding rna fendrr regulates ifnγ-induced m1 phenotype in macrophages |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426844/ https://www.ncbi.nlm.nih.gov/pubmed/32792604 http://dx.doi.org/10.1038/s41598-020-70633-7 |
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