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Compression and stretch sensitive submucosal neurons of the porcine and human colon
The pig is commonly believed to be a relevant model for human gut functions—however, there are only a few comparative studies and none on neural control mechanisms. To address this lack we identified as one central aspect mechanosensitive enteric neurons (MEN) in porcine and human colon. We used neu...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7428018/ https://www.ncbi.nlm.nih.gov/pubmed/32796868 http://dx.doi.org/10.1038/s41598-020-70216-6 |
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author | Filzmayer, Anna Katharina Elfers, Kristin Michel, Klaus Buhner, Sabine Zeller, Florian Demir, Ihsan Ekin Theisen, Jörg Schemann, Michael Mazzuoli-Weber, Gemma |
author_facet | Filzmayer, Anna Katharina Elfers, Kristin Michel, Klaus Buhner, Sabine Zeller, Florian Demir, Ihsan Ekin Theisen, Jörg Schemann, Michael Mazzuoli-Weber, Gemma |
author_sort | Filzmayer, Anna Katharina |
collection | PubMed |
description | The pig is commonly believed to be a relevant model for human gut functions—however, there are only a few comparative studies and none on neural control mechanisms. To address this lack we identified as one central aspect mechanosensitive enteric neurons (MEN) in porcine and human colon. We used neuroimaging techniques to record responses to tensile or compressive forces in submucous neurons. Compression and stretch caused Ca-transients and immediate spike discharge in 5–11% of porcine and 15–24% of human enteric neurons. The majority of these MEN exclusively responded to either stimulus quality but about 9% responded to both. Most of the MEN expressed choline acetyltransferase and substance P; nitric oxide synthase-positive MEN primarily occurred in distal colon. The findings reveal common features of MEN in human and pig colon which we interpret as a result of species-independent evolutionary conservation rather than a specific functional proximity between the two species. |
format | Online Article Text |
id | pubmed-7428018 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-74280182020-08-18 Compression and stretch sensitive submucosal neurons of the porcine and human colon Filzmayer, Anna Katharina Elfers, Kristin Michel, Klaus Buhner, Sabine Zeller, Florian Demir, Ihsan Ekin Theisen, Jörg Schemann, Michael Mazzuoli-Weber, Gemma Sci Rep Article The pig is commonly believed to be a relevant model for human gut functions—however, there are only a few comparative studies and none on neural control mechanisms. To address this lack we identified as one central aspect mechanosensitive enteric neurons (MEN) in porcine and human colon. We used neuroimaging techniques to record responses to tensile or compressive forces in submucous neurons. Compression and stretch caused Ca-transients and immediate spike discharge in 5–11% of porcine and 15–24% of human enteric neurons. The majority of these MEN exclusively responded to either stimulus quality but about 9% responded to both. Most of the MEN expressed choline acetyltransferase and substance P; nitric oxide synthase-positive MEN primarily occurred in distal colon. The findings reveal common features of MEN in human and pig colon which we interpret as a result of species-independent evolutionary conservation rather than a specific functional proximity between the two species. Nature Publishing Group UK 2020-08-14 /pmc/articles/PMC7428018/ /pubmed/32796868 http://dx.doi.org/10.1038/s41598-020-70216-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Filzmayer, Anna Katharina Elfers, Kristin Michel, Klaus Buhner, Sabine Zeller, Florian Demir, Ihsan Ekin Theisen, Jörg Schemann, Michael Mazzuoli-Weber, Gemma Compression and stretch sensitive submucosal neurons of the porcine and human colon |
title | Compression and stretch sensitive submucosal neurons of the porcine and human colon |
title_full | Compression and stretch sensitive submucosal neurons of the porcine and human colon |
title_fullStr | Compression and stretch sensitive submucosal neurons of the porcine and human colon |
title_full_unstemmed | Compression and stretch sensitive submucosal neurons of the porcine and human colon |
title_short | Compression and stretch sensitive submucosal neurons of the porcine and human colon |
title_sort | compression and stretch sensitive submucosal neurons of the porcine and human colon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7428018/ https://www.ncbi.nlm.nih.gov/pubmed/32796868 http://dx.doi.org/10.1038/s41598-020-70216-6 |
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