Cargando…
Growth, physiological and transcriptomic analysis of the perennial ryegrass Lolium perenne in response to saline stress
Salinization of soil is a global environmental concern. To bioremediate or use saline-alkali lands, most studies focused on screening of halophytes and breeding of saline-tolerant non-halophyte vegetables. Seldom studies investigated effects of salinity on general landscape plants, which are importa...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7428229/ https://www.ncbi.nlm.nih.gov/pubmed/32874657 http://dx.doi.org/10.1098/rsos.200637 |
_version_ | 1783571028809613312 |
---|---|
author | Xu, Hai-Shun Guo, Su-Ming Zhu, Lin Xing, Jin-Cheng |
author_facet | Xu, Hai-Shun Guo, Su-Ming Zhu, Lin Xing, Jin-Cheng |
author_sort | Xu, Hai-Shun |
collection | PubMed |
description | Salinization of soil is a global environmental concern. To bioremediate or use saline-alkali lands, most studies focused on screening of halophytes and breeding of saline-tolerant non-halophyte vegetables. Seldom studies investigated effects of salinity on general landscape plants, which are important for landscape construction in urban areas. In the present study, effects of salinity on seed germination and seedling growth of the perennial ryegrass Lolium perenne were investigated. The final seed germination rate was not affected at salinity up to 6.4‰. Partial seedlings wilted in all saline treatments and the mortality of L. perenne was positively correlated with salinity. Treatments with salinity equal to or lower than 1.6‰ did not affect length and dry weight of shoot and root. These results suggested that L. perenne could be sowed and then grow well in low-salinity areas. To explore the underlying physiological mechanisms, contents of photosynthetic pigments and antioxidant indices were determined. The results showed that contents of chlorophyll a, b and carotenoid significantly decreased in all saline treatments, in comparison to the control. Similarly, activities of superoxide dismutase and peroxidase decreased and contents of glutathione and malondialdehyde increased in saline treatments. Additionally, transcriptome analysis identified 792 differentially expressed genes (DEGs) in L. perenne shoots between 6.4‰ saline treatment and the control. Compared with the control, genes in relation to iron transportation and amino acid metabolism were downregulated, but genes participating in energy metabolism were upregulated. These changes would inhibit toxicity of ion accumulation and provide more energy for plants to resist saline stress. |
format | Online Article Text |
id | pubmed-7428229 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-74282292020-08-31 Growth, physiological and transcriptomic analysis of the perennial ryegrass Lolium perenne in response to saline stress Xu, Hai-Shun Guo, Su-Ming Zhu, Lin Xing, Jin-Cheng R Soc Open Sci Ecology, Conservation, and Global Change Biology Salinization of soil is a global environmental concern. To bioremediate or use saline-alkali lands, most studies focused on screening of halophytes and breeding of saline-tolerant non-halophyte vegetables. Seldom studies investigated effects of salinity on general landscape plants, which are important for landscape construction in urban areas. In the present study, effects of salinity on seed germination and seedling growth of the perennial ryegrass Lolium perenne were investigated. The final seed germination rate was not affected at salinity up to 6.4‰. Partial seedlings wilted in all saline treatments and the mortality of L. perenne was positively correlated with salinity. Treatments with salinity equal to or lower than 1.6‰ did not affect length and dry weight of shoot and root. These results suggested that L. perenne could be sowed and then grow well in low-salinity areas. To explore the underlying physiological mechanisms, contents of photosynthetic pigments and antioxidant indices were determined. The results showed that contents of chlorophyll a, b and carotenoid significantly decreased in all saline treatments, in comparison to the control. Similarly, activities of superoxide dismutase and peroxidase decreased and contents of glutathione and malondialdehyde increased in saline treatments. Additionally, transcriptome analysis identified 792 differentially expressed genes (DEGs) in L. perenne shoots between 6.4‰ saline treatment and the control. Compared with the control, genes in relation to iron transportation and amino acid metabolism were downregulated, but genes participating in energy metabolism were upregulated. These changes would inhibit toxicity of ion accumulation and provide more energy for plants to resist saline stress. The Royal Society 2020-07-08 /pmc/articles/PMC7428229/ /pubmed/32874657 http://dx.doi.org/10.1098/rsos.200637 Text en © 2020 The Authors. http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Ecology, Conservation, and Global Change Biology Xu, Hai-Shun Guo, Su-Ming Zhu, Lin Xing, Jin-Cheng Growth, physiological and transcriptomic analysis of the perennial ryegrass Lolium perenne in response to saline stress |
title | Growth, physiological and transcriptomic analysis of the perennial ryegrass Lolium perenne in response to saline stress |
title_full | Growth, physiological and transcriptomic analysis of the perennial ryegrass Lolium perenne in response to saline stress |
title_fullStr | Growth, physiological and transcriptomic analysis of the perennial ryegrass Lolium perenne in response to saline stress |
title_full_unstemmed | Growth, physiological and transcriptomic analysis of the perennial ryegrass Lolium perenne in response to saline stress |
title_short | Growth, physiological and transcriptomic analysis of the perennial ryegrass Lolium perenne in response to saline stress |
title_sort | growth, physiological and transcriptomic analysis of the perennial ryegrass lolium perenne in response to saline stress |
topic | Ecology, Conservation, and Global Change Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7428229/ https://www.ncbi.nlm.nih.gov/pubmed/32874657 http://dx.doi.org/10.1098/rsos.200637 |
work_keys_str_mv | AT xuhaishun growthphysiologicalandtranscriptomicanalysisoftheperennialryegrassloliumperenneinresponsetosalinestress AT guosuming growthphysiologicalandtranscriptomicanalysisoftheperennialryegrassloliumperenneinresponsetosalinestress AT zhulin growthphysiologicalandtranscriptomicanalysisoftheperennialryegrassloliumperenneinresponsetosalinestress AT xingjincheng growthphysiologicalandtranscriptomicanalysisoftheperennialryegrassloliumperenneinresponsetosalinestress |