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Bioenergetics Consequences of Mitochondrial Transplantation in Cardiomyocytes

BACKGROUND: Mitochondrial transplantation has been recently explored for treatment of very ill cardiac patients. However, little is known about the intracellular consequences of mitochondrial transplantation. This study aims to assess the bioenergetics consequences of mitochondrial transplantation i...

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Autores principales: Ali Pour, Paria, Kenney, M. Cristina, Kheradvar, Arash
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7428632/
https://www.ncbi.nlm.nih.gov/pubmed/32200731
http://dx.doi.org/10.1161/JAHA.119.014501
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author Ali Pour, Paria
Kenney, M. Cristina
Kheradvar, Arash
author_facet Ali Pour, Paria
Kenney, M. Cristina
Kheradvar, Arash
author_sort Ali Pour, Paria
collection PubMed
description BACKGROUND: Mitochondrial transplantation has been recently explored for treatment of very ill cardiac patients. However, little is known about the intracellular consequences of mitochondrial transplantation. This study aims to assess the bioenergetics consequences of mitochondrial transplantation into normal cardiomyocytes in the short and long term. METHODS AND RESULTS: We first established the feasibility of autologous, non‐autologous, and interspecies mitochondrial transplantation. Then we quantitated the bioenergetics consequences of non‐autologous mitochondrial transplantation into cardiomyocytes up to 28 days using a Seahorse Extracellular Flux Analyzer. Compared with the control, we observed a statistically significant improvement in basal respiration and ATP production 2‐day post‐transplantation, accompanied by an increase in maximal respiration and spare respiratory capacity, although not statistically significantly. However, these initial improvements were short‐lived and the bioenergetics advantages return to the baseline level in subsequent time points. CONCLUSIONS: This study, for the first time, shows that transplantation of non‐autologous mitochondria from healthy skeletal muscle cells into normal cardiomyocytes leads to short‐term improvement of bioenergetics indicating “supercharged” state. However, over time these improved effects disappear, which suggests transplantation of mitochondria may have a potential application in settings where there is an acute stress.
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spelling pubmed-74286322020-08-17 Bioenergetics Consequences of Mitochondrial Transplantation in Cardiomyocytes Ali Pour, Paria Kenney, M. Cristina Kheradvar, Arash J Am Heart Assoc Original Research BACKGROUND: Mitochondrial transplantation has been recently explored for treatment of very ill cardiac patients. However, little is known about the intracellular consequences of mitochondrial transplantation. This study aims to assess the bioenergetics consequences of mitochondrial transplantation into normal cardiomyocytes in the short and long term. METHODS AND RESULTS: We first established the feasibility of autologous, non‐autologous, and interspecies mitochondrial transplantation. Then we quantitated the bioenergetics consequences of non‐autologous mitochondrial transplantation into cardiomyocytes up to 28 days using a Seahorse Extracellular Flux Analyzer. Compared with the control, we observed a statistically significant improvement in basal respiration and ATP production 2‐day post‐transplantation, accompanied by an increase in maximal respiration and spare respiratory capacity, although not statistically significantly. However, these initial improvements were short‐lived and the bioenergetics advantages return to the baseline level in subsequent time points. CONCLUSIONS: This study, for the first time, shows that transplantation of non‐autologous mitochondria from healthy skeletal muscle cells into normal cardiomyocytes leads to short‐term improvement of bioenergetics indicating “supercharged” state. However, over time these improved effects disappear, which suggests transplantation of mitochondria may have a potential application in settings where there is an acute stress. John Wiley and Sons Inc. 2020-03-23 /pmc/articles/PMC7428632/ /pubmed/32200731 http://dx.doi.org/10.1161/JAHA.119.014501 Text en © 2020 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Research
Ali Pour, Paria
Kenney, M. Cristina
Kheradvar, Arash
Bioenergetics Consequences of Mitochondrial Transplantation in Cardiomyocytes
title Bioenergetics Consequences of Mitochondrial Transplantation in Cardiomyocytes
title_full Bioenergetics Consequences of Mitochondrial Transplantation in Cardiomyocytes
title_fullStr Bioenergetics Consequences of Mitochondrial Transplantation in Cardiomyocytes
title_full_unstemmed Bioenergetics Consequences of Mitochondrial Transplantation in Cardiomyocytes
title_short Bioenergetics Consequences of Mitochondrial Transplantation in Cardiomyocytes
title_sort bioenergetics consequences of mitochondrial transplantation in cardiomyocytes
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7428632/
https://www.ncbi.nlm.nih.gov/pubmed/32200731
http://dx.doi.org/10.1161/JAHA.119.014501
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