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Determination of Cassiarin A Level of Cassia siamea Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method

Cassia siamea leaf has been proven in vitro and in vivo to have a strong antimalarial activity with Cassiarin A as its active compound. To obtain a source of C. siamea medicinal plant with high level of active antimalarial compound (Cassiarin A), a valid method for determining Cassiarin A level is n...

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Autores principales: Ekasari, Wiwied, Widiyastuti, Yuli, Subositi, Dyah, Hamsidi, Rini, Widyawaruyanti, Aty, Basuki, Sukmawati, Setyawan, Dwi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7428929/
https://www.ncbi.nlm.nih.gov/pubmed/32831808
http://dx.doi.org/10.1155/2020/7367836
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author Ekasari, Wiwied
Widiyastuti, Yuli
Subositi, Dyah
Hamsidi, Rini
Widyawaruyanti, Aty
Basuki, Sukmawati
Setyawan, Dwi
author_facet Ekasari, Wiwied
Widiyastuti, Yuli
Subositi, Dyah
Hamsidi, Rini
Widyawaruyanti, Aty
Basuki, Sukmawati
Setyawan, Dwi
author_sort Ekasari, Wiwied
collection PubMed
description Cassia siamea leaf has been proven in vitro and in vivo to have a strong antimalarial activity with Cassiarin A as its active compound. To obtain a source of C. siamea medicinal plant with high level of active antimalarial compound (Cassiarin A), a valid method for determining Cassiarin A level is needed. For this reason, this research conducts the validation of the Cassiarin A content with determination method using thin-layer chromatography (TLC) densitometry which includes the determination of selectivity (Rs), linearity (r), accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). Cassiarin A was chromatographed on silica gel 60 F(254) TLC plate using chloroform : ethanol (85 : 15 v/v) as a mobile phase. Cassiarin A was quantified by densitometric analysis at 368 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r = 0.9995. The method was validated for precision, recovery, repeatability. The minimum detectable amount was found to be 0.0027 μg/spot, whereas the limit of quantitation was found to be 0.008 μg/spot. The results of this validation are then used to determine the Cassiarin A level of C. siamea leaf from various regions in Indonesia. Based on the results of the study, it can be concluded that the TLC-densitometry method can be used to determine level of the Cassiarin A compound with the advantages of being fast, easy, accurate, and inexpensive. In addition, it showed that C. siamea leaves from Pacitan have the highest level of Cassiarin A compared to other areas studied.
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spelling pubmed-74289292020-08-20 Determination of Cassiarin A Level of Cassia siamea Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method Ekasari, Wiwied Widiyastuti, Yuli Subositi, Dyah Hamsidi, Rini Widyawaruyanti, Aty Basuki, Sukmawati Setyawan, Dwi ScientificWorldJournal Research Article Cassia siamea leaf has been proven in vitro and in vivo to have a strong antimalarial activity with Cassiarin A as its active compound. To obtain a source of C. siamea medicinal plant with high level of active antimalarial compound (Cassiarin A), a valid method for determining Cassiarin A level is needed. For this reason, this research conducts the validation of the Cassiarin A content with determination method using thin-layer chromatography (TLC) densitometry which includes the determination of selectivity (Rs), linearity (r), accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). Cassiarin A was chromatographed on silica gel 60 F(254) TLC plate using chloroform : ethanol (85 : 15 v/v) as a mobile phase. Cassiarin A was quantified by densitometric analysis at 368 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r = 0.9995. The method was validated for precision, recovery, repeatability. The minimum detectable amount was found to be 0.0027 μg/spot, whereas the limit of quantitation was found to be 0.008 μg/spot. The results of this validation are then used to determine the Cassiarin A level of C. siamea leaf from various regions in Indonesia. Based on the results of the study, it can be concluded that the TLC-densitometry method can be used to determine level of the Cassiarin A compound with the advantages of being fast, easy, accurate, and inexpensive. In addition, it showed that C. siamea leaves from Pacitan have the highest level of Cassiarin A compared to other areas studied. Hindawi 2020-08-07 /pmc/articles/PMC7428929/ /pubmed/32831808 http://dx.doi.org/10.1155/2020/7367836 Text en Copyright © 2020 Wiwied Ekasari et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ekasari, Wiwied
Widiyastuti, Yuli
Subositi, Dyah
Hamsidi, Rini
Widyawaruyanti, Aty
Basuki, Sukmawati
Setyawan, Dwi
Determination of Cassiarin A Level of Cassia siamea Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method
title Determination of Cassiarin A Level of Cassia siamea Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method
title_full Determination of Cassiarin A Level of Cassia siamea Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method
title_fullStr Determination of Cassiarin A Level of Cassia siamea Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method
title_full_unstemmed Determination of Cassiarin A Level of Cassia siamea Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method
title_short Determination of Cassiarin A Level of Cassia siamea Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method
title_sort determination of cassiarin a level of cassia siamea leaf obtained from various regions in indonesia using the tlc-densitometry method
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7428929/
https://www.ncbi.nlm.nih.gov/pubmed/32831808
http://dx.doi.org/10.1155/2020/7367836
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