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Maximizing transcription of nucleic acids with efficient T7 promoters
In vitro transcription using T7 bacteriophage polymerase is widely used in molecular biology. Here, we use 5′RACE-Seq to screen a randomized initially transcribed region of the T7 promoter for cross-talk with transcriptional activity. We reveal that sequences from position +4 to +8 downstream of the...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429497/ https://www.ncbi.nlm.nih.gov/pubmed/32796901 http://dx.doi.org/10.1038/s42003-020-01167-x |