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Donor hearts in the Sydney Heart Bank: reliable control but is it ‘normal’ heart?
Human heart samples from the Sydney Heart Bank have become a de facto standard against which others can be measured. Crucially, the heart bank contains a lot of donor heart material: for most researchers this is the hardest to obtain and yet is necessary since we can only study the pathological huma...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429572/ https://www.ncbi.nlm.nih.gov/pubmed/32691299 http://dx.doi.org/10.1007/s12551-020-00740-2 |
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author | Marston, Steven Jacques, Adam Bayliss, Christopher Dyer, Emma Memo, Massimiliano Papadaki, Maria Messer, Andrew |
author_facet | Marston, Steven Jacques, Adam Bayliss, Christopher Dyer, Emma Memo, Massimiliano Papadaki, Maria Messer, Andrew |
author_sort | Marston, Steven |
collection | PubMed |
description | Human heart samples from the Sydney Heart Bank have become a de facto standard against which others can be measured. Crucially, the heart bank contains a lot of donor heart material: for most researchers this is the hardest to obtain and yet is necessary since we can only study the pathological human heart in comparison with a control, preferably a normal heart sample. It is not generally realised how important the control is for human heart studies. We review our studies on donor heart samples. We report the results obtained with 17 different donor samples collected from 1994 to 2011 and measured from 2005 to 2015 by our standard methodology for in vitro motility and troponin I phosphorylation measurements. The donor heart sample parameters are consistent between the hearts, over time and with different operators indicating that Sydney Heart Bank donor hearts are a valid baseline control for comparison with pathological heart samples. We also discuss to what extent donor heart samples are representative of the normal heart. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12551-020-00740-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7429572 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-74295722020-08-20 Donor hearts in the Sydney Heart Bank: reliable control but is it ‘normal’ heart? Marston, Steven Jacques, Adam Bayliss, Christopher Dyer, Emma Memo, Massimiliano Papadaki, Maria Messer, Andrew Biophys Rev Review Human heart samples from the Sydney Heart Bank have become a de facto standard against which others can be measured. Crucially, the heart bank contains a lot of donor heart material: for most researchers this is the hardest to obtain and yet is necessary since we can only study the pathological human heart in comparison with a control, preferably a normal heart sample. It is not generally realised how important the control is for human heart studies. We review our studies on donor heart samples. We report the results obtained with 17 different donor samples collected from 1994 to 2011 and measured from 2005 to 2015 by our standard methodology for in vitro motility and troponin I phosphorylation measurements. The donor heart sample parameters are consistent between the hearts, over time and with different operators indicating that Sydney Heart Bank donor hearts are a valid baseline control for comparison with pathological heart samples. We also discuss to what extent donor heart samples are representative of the normal heart. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12551-020-00740-2) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2020-07-20 /pmc/articles/PMC7429572/ /pubmed/32691299 http://dx.doi.org/10.1007/s12551-020-00740-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Review Marston, Steven Jacques, Adam Bayliss, Christopher Dyer, Emma Memo, Massimiliano Papadaki, Maria Messer, Andrew Donor hearts in the Sydney Heart Bank: reliable control but is it ‘normal’ heart? |
title | Donor hearts in the Sydney Heart Bank: reliable control but is it ‘normal’ heart? |
title_full | Donor hearts in the Sydney Heart Bank: reliable control but is it ‘normal’ heart? |
title_fullStr | Donor hearts in the Sydney Heart Bank: reliable control but is it ‘normal’ heart? |
title_full_unstemmed | Donor hearts in the Sydney Heart Bank: reliable control but is it ‘normal’ heart? |
title_short | Donor hearts in the Sydney Heart Bank: reliable control but is it ‘normal’ heart? |
title_sort | donor hearts in the sydney heart bank: reliable control but is it ‘normal’ heart? |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429572/ https://www.ncbi.nlm.nih.gov/pubmed/32691299 http://dx.doi.org/10.1007/s12551-020-00740-2 |
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