Cargando…

A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice

Double-strand breaks (DSBs) are the most toxic type of DNA lesions. Cells repair these lesions using either end protection- or end resection-coupled mechanisms. To study DSB repair choice, we present the Color Assay Tracing-Repair (CAT-R) to simultaneously quantify DSB repair via end protection and...

Descripción completa

Detalles Bibliográficos
Autores principales:	Roidos, Paris, Sungalee, Stephanie, Benfatto, Salvatore, Serçin, Özdemirhan, Stütz, Adrian M., Abdollahi, Amir, Mauer, Jan, Zenke, Frank T., Korbel, Jan O., Mardin, Balca R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429917/ https://www.ncbi.nlm.nih.gov/pubmed/32796846 http://dx.doi.org/10.1038/s41467-020-17962-3

Ejemplares similares

Uncovering cancer vulnerabilities by machine learning prediction of synthetic lethality
por: Benfatto, Salvatore, et al.
Publicado: (2021)

A solid‐phase transfection platform for arrayed CRISPR screens
por: Serçin, Özdemirhan, et al.
Publicado: (2019)

Systematic Identification of Determinants for Single-Strand Annealing-Mediated Deletion Formation in Saccharomyces cerevisiae
por: Segura-Wang, Maia, et al.
Publicado: (2017)

Genome-wide Screens Implicate Loss of Cullin Ring Ligase 3 in Persistent Proliferation and Genome Instability in TP53-Deficient Cells
por: Drainas, Alexandros P., et al.
Publicado: (2020)

The interplay of DNA repair context with target sequence predictably biasses Cas9-generated mutations
por: Pallaseni, Ananth, et al.
Publicado: (2023)

Breaking the ties that bind: New advances in centrosome biology
por: Mardin, Balca R., et al.
Publicado: (2012)

Methods Favoring Homology-Directed Repair Choice in Response to CRISPR/Cas9 Induced-Double Strand Breaks
por: Yang, Han, et al.
Publicado: (2020)

Pan-cancer analysis distinguishes transcriptional changes of aneuploidy from proliferation
por: Buccitelli, Christopher, et al.
Publicado: (2017)

CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks
por: Cofsky, Joshua C, et al.
Publicado: (2020)

Target residence of Cas9-sgRNA influences DNA double-strand break repair pathway choices in CRISPR/Cas9 genome editing
por: Liu, Si-Cheng, et al.
Publicado: (2022)

Somatic structural variant formation is guided by and influences genome architecture
por: Sidiropoulos, Nikos, et al.
Publicado: (2022)

CRISPR/Cas9-Induced Double-Strand Break Repair in Arabidopsis Nonhomologous End-Joining Mutants
por: Shen, Hexi, et al.
Publicado: (2016)

Host Double Strand Break Repair Generates HIV-1 Strains Resistant to CRISPR/Cas9
por: Yoder, Kristine E., et al.
Publicado: (2016)

Massively parallel profiling and predictive modeling of the outcomes of CRISPR/Cas9-mediated double-strand break repair
por: Chen, Wei, et al.
Publicado: (2019)

Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in Leishmania
por: Zhang, Wen-Wei, et al.
Publicado: (2019)

Negative Selection and Chromosome Instability Induced by Mad2 Overexpression Delay Breast Cancer but Facilitate Oncogene-Independent Outgrowth
por: Rowald, Konstantina, et al.
Publicado: (2016)

A robust CRISPR–Cas9-based fluorescent reporter assay for the detection and quantification of DNA double-strand break repair
por: Eki, Rebeka, et al.
Publicado: (2020)

CRISPR base editors: genome editing without double-stranded breaks
por: Eid, Ayman, et al.
Publicado: (2018)

IncC conjugative plasmids and SXT/R391 elements repair double-strand breaks caused by CRISPR–Cas during conjugation
por: Roy, David, et al.
Publicado: (2020)

CRISPR-Cas12a induced DNA double-strand breaks are repaired by multiple pathways with different mutation profiles in Magnaporthe oryzae
por: Huang, Jun, et al.
Publicado: (2022)

Breaking-Cas—interactive design of guide RNAs for CRISPR-Cas experiments for ENSEMBL genomes
por: Oliveros, Juan C., et al.
Publicado: (2016)

A cell-based model system links chromothripsis with hyperploidy
por: Mardin, Balca R, et al.
Publicado: (2015)

Targeting DNA polymerase to DNA double-strand breaks reduces DNA deletion size and increases templated insertions generated by CRISPR/Cas9
por: Yoo, Kyung W, et al.
Publicado: (2022)

Kinetics and Fidelity of the Repair of Cas9-Induced Double-Strand DNA Breaks
por: Brinkman, Eva K., et al.
Publicado: (2018)

Break Breast Cancer Addiction by CRISPR/Cas9 Genome Editing
por: Yang, Haitao, et al.
Publicado: (2018)

Scalable CRISPR-Cas9 chemical genetic screens in non-transformed human cells
por: Lin, Kevin, et al.
Publicado: (2022)

Concurrent live imaging of DNA double-strand break repair and cell-cycle progression by CRISPR/Cas9-mediated knock-in of a tricistronic vector
por: Otsuka, Kensuke, et al.
Publicado: (2018)

Rates of homology directed repair of CRISPR-Cas9 induced double strand breaks are lower in naïve compared to primed human pluripotent stem cells
por: Dodsworth, Benjamin T., et al.
Publicado: (2020)

CRISPR-induced double-strand breaks trigger recombination between homologous chromosome arms
por: Brunner, Erich, et al.
Publicado: (2019)

Multiplexable and Biocomputational Virus Detection by CRISPR-Cas9-Mediated Strand Displacement
por: Márquez-Costa, Rosa, et al.
Publicado: (2023)

DNA double-strand break-free CRISPR interference delays Huntington’s disease progression in mice
por: Seo, Jung Hwa, et al.
Publicado: (2023)

CRISPR–Cas12a-mediated DNA clamping triggers target-strand cleavage
por: Naqvi, Mohsin M., et al.
Publicado: (2022)

Marker-free quantification of repair pathway utilization at Cas9-induced double-strand breaks
por: Feng, Wanjuan, et al.
Publicado: (2021)

Upgrading of efficient and scalable CRISPR–Cas-mediated technology for genetic engineering in thermophilic fungus Myceliophthora thermophila
por: Liu, Qian, et al.
Publicado: (2019)

Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases
por: Huang, Hongxin, et al.
Publicado: (2021)

Systems approaches identify the consequences of monosomy in somatic human cells
por: Chunduri, Narendra Kumar, et al.
Publicado: (2021)

Regulation of Gene Editing Activity Directed by Single-Stranded Oligonucleotides and CRISPR/Cas9 Systems
por: Bialk, Pawel, et al.
Publicado: (2015)

A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
por: Zhou, Wenhua, et al.
Publicado: (2018)

Rheostatic Control of Cas9-Mediated DNA Double Strand Break (DSB) Generation and Genome Editing
por: Rose, John C., et al.
Publicado: (2017)

Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance
por: Schep, Ruben, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_9c4pes0h74elto9csqp69nb4t0