Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids

Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal o...

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Detalles Bibliográficos
Autores principales: Scholler, Jules, Groux, Kassandra, Goureau, Olivier, Sahel, José-Alain, Fink, Mathias, Reichman, Sacha, Boccara, Claude, Grieve, Kate
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429964/
https://www.ncbi.nlm.nih.gov/pubmed/32864115
http://dx.doi.org/10.1038/s41377-020-00375-8
Descripción
Sumario:Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal organoids. Coloured images with an endogenous contrast linked to organelle motility are generated, with submicrometre spatial resolution and millisecond temporal resolution, creating a way to identify specific cell types in living tissue via their function.

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