Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids

Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal o...

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Detalles Bibliográficos
Autores principales: Scholler, Jules, Groux, Kassandra, Goureau, Olivier, Sahel, José-Alain, Fink, Mathias, Reichman, Sacha, Boccara, Claude, Grieve, Kate
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429964/
https://www.ncbi.nlm.nih.gov/pubmed/32864115
http://dx.doi.org/10.1038/s41377-020-00375-8
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author Scholler, Jules
Groux, Kassandra
Goureau, Olivier
Sahel, José-Alain
Fink, Mathias
Reichman, Sacha
Boccara, Claude
Grieve, Kate
author_facet Scholler, Jules
Groux, Kassandra
Goureau, Olivier
Sahel, José-Alain
Fink, Mathias
Reichman, Sacha
Boccara, Claude
Grieve, Kate
author_sort Scholler, Jules
collection PubMed
description Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal organoids. Coloured images with an endogenous contrast linked to organelle motility are generated, with submicrometre spatial resolution and millisecond temporal resolution, creating a way to identify specific cell types in living tissue via their function.
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spelling pubmed-74299642020-08-27 Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids Scholler, Jules Groux, Kassandra Goureau, Olivier Sahel, José-Alain Fink, Mathias Reichman, Sacha Boccara, Claude Grieve, Kate Light Sci Appl Letter Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal organoids. Coloured images with an endogenous contrast linked to organelle motility are generated, with submicrometre spatial resolution and millisecond temporal resolution, creating a way to identify specific cell types in living tissue via their function. Nature Publishing Group UK 2020-08-17 /pmc/articles/PMC7429964/ /pubmed/32864115 http://dx.doi.org/10.1038/s41377-020-00375-8 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Letter
Scholler, Jules
Groux, Kassandra
Goureau, Olivier
Sahel, José-Alain
Fink, Mathias
Reichman, Sacha
Boccara, Claude
Grieve, Kate
Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids
title Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids
title_full Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids
title_fullStr Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids
title_full_unstemmed Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids
title_short Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids
title_sort dynamic full-field optical coherence tomography: 3d live-imaging of retinal organoids
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429964/
https://www.ncbi.nlm.nih.gov/pubmed/32864115
http://dx.doi.org/10.1038/s41377-020-00375-8
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