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Revisiting the window of opportunity for cotranscriptional splicing in budding yeast

We reported previously that, in budding yeast, transcription rate affects both the efficiency and fidelity of pre-mRNA splicing, especially of ribosomal protein transcripts. Here, we report that the majority of ribosomal protein transcripts with non-consensus 5′ splice sites are spliced less efficie...

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Detalles Bibliográficos
Autores principales: Aslanzadeh, Vahid, Beggs, Jean D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430680/
https://www.ncbi.nlm.nih.gov/pubmed/32439718
http://dx.doi.org/10.1261/rna.075895.120
Descripción
Sumario:We reported previously that, in budding yeast, transcription rate affects both the efficiency and fidelity of pre-mRNA splicing, especially of ribosomal protein transcripts. Here, we report that the majority of ribosomal protein transcripts with non-consensus 5′ splice sites are spliced less efficiently when transcription is faster, and more efficiently with slower transcription. These results support the “window of opportunity” model, and we suggest a possible mechanism to explain these findings.