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Phylogenetic evidence for the invasion of a commercialized European Phasmarhabditis hermaphrodita lineage into North America and New Zealand

Biological control (biocontrol) as a component of pest management strategies reduces reliance on synthetic chemicals, and seemingly offers a natural approach that minimizes environmental impact. However, introducing a new organism to new environments as a classical biocontrol agent can have broad an...

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Autores principales: Howe, Dana K., Ha, Anh D., Colton, Andrew, De Ley, Irma Tandingan, Rae, Robbie G., Ross, Jenna, Wilson, Michael, Nermut, Jiří, Zhao, Zhongying, Mc Donnell, Rory J., Denver, Dee R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430733/
https://www.ncbi.nlm.nih.gov/pubmed/32804938
http://dx.doi.org/10.1371/journal.pone.0237249
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author Howe, Dana K.
Ha, Anh D.
Colton, Andrew
De Ley, Irma Tandingan
Rae, Robbie G.
Ross, Jenna
Wilson, Michael
Nermut, Jiří
Zhao, Zhongying
Mc Donnell, Rory J.
Denver, Dee R.
author_facet Howe, Dana K.
Ha, Anh D.
Colton, Andrew
De Ley, Irma Tandingan
Rae, Robbie G.
Ross, Jenna
Wilson, Michael
Nermut, Jiří
Zhao, Zhongying
Mc Donnell, Rory J.
Denver, Dee R.
author_sort Howe, Dana K.
collection PubMed
description Biological control (biocontrol) as a component of pest management strategies reduces reliance on synthetic chemicals, and seemingly offers a natural approach that minimizes environmental impact. However, introducing a new organism to new environments as a classical biocontrol agent can have broad and unanticipated biodiversity effects and conservation consequences. Nematodes are currently used in a variety of commercial biocontrol applications, including the use of Phasmarhabditis hermaphrodita as an agent targeting pest slug and snail species. This species was originally discovered in Germany, and is generally thought to have European origins. P. hermaphrodita is sold under the trade name Nemaslug®, and is available only in European markets. However, this nematode species was discovered in New Zealand and the western United States, though its specific origins remained unclear. In this study, we analyzed 45 nematode strains representing eight different Phasmarhabditis species, collected from nine countries around the world. A segment of nematode mitochondrial DNA (mtDNA) was sequenced and subjected to phylogenetic analyses. Our mtDNA phylogenies were overall consistent with previous analyses based on nuclear ribosomal RNA (rRNA) loci. The recently discovered P. hermaphrodita strains in New Zealand and the United States had mtDNA haplotypes nearly identical to that of Nemaslug®, and these were placed together in an intraspecific monophyletic clade with high support in maximum likelihood and Bayesian analyses. We also examined bacteria that co-cultured with the nematode strains isolated in Oregon, USA, by analyzing 16S rRNA sequences. Eight different bacterial genera were found to associate with these nematodes, though Moraxella osloensis, the bacteria species used in the Nemaslug® formulation, was not detected. This study provided evidence that nematodes deriving from the Nemaslug® biocontrol product have invaded countries where its use is prohibited by regulatory agencies and not commercially available.
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spelling pubmed-74307332020-08-20 Phylogenetic evidence for the invasion of a commercialized European Phasmarhabditis hermaphrodita lineage into North America and New Zealand Howe, Dana K. Ha, Anh D. Colton, Andrew De Ley, Irma Tandingan Rae, Robbie G. Ross, Jenna Wilson, Michael Nermut, Jiří Zhao, Zhongying Mc Donnell, Rory J. Denver, Dee R. PLoS One Research Article Biological control (biocontrol) as a component of pest management strategies reduces reliance on synthetic chemicals, and seemingly offers a natural approach that minimizes environmental impact. However, introducing a new organism to new environments as a classical biocontrol agent can have broad and unanticipated biodiversity effects and conservation consequences. Nematodes are currently used in a variety of commercial biocontrol applications, including the use of Phasmarhabditis hermaphrodita as an agent targeting pest slug and snail species. This species was originally discovered in Germany, and is generally thought to have European origins. P. hermaphrodita is sold under the trade name Nemaslug®, and is available only in European markets. However, this nematode species was discovered in New Zealand and the western United States, though its specific origins remained unclear. In this study, we analyzed 45 nematode strains representing eight different Phasmarhabditis species, collected from nine countries around the world. A segment of nematode mitochondrial DNA (mtDNA) was sequenced and subjected to phylogenetic analyses. Our mtDNA phylogenies were overall consistent with previous analyses based on nuclear ribosomal RNA (rRNA) loci. The recently discovered P. hermaphrodita strains in New Zealand and the United States had mtDNA haplotypes nearly identical to that of Nemaslug®, and these were placed together in an intraspecific monophyletic clade with high support in maximum likelihood and Bayesian analyses. We also examined bacteria that co-cultured with the nematode strains isolated in Oregon, USA, by analyzing 16S rRNA sequences. Eight different bacterial genera were found to associate with these nematodes, though Moraxella osloensis, the bacteria species used in the Nemaslug® formulation, was not detected. This study provided evidence that nematodes deriving from the Nemaslug® biocontrol product have invaded countries where its use is prohibited by regulatory agencies and not commercially available. Public Library of Science 2020-08-17 /pmc/articles/PMC7430733/ /pubmed/32804938 http://dx.doi.org/10.1371/journal.pone.0237249 Text en © 2020 Howe et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Howe, Dana K.
Ha, Anh D.
Colton, Andrew
De Ley, Irma Tandingan
Rae, Robbie G.
Ross, Jenna
Wilson, Michael
Nermut, Jiří
Zhao, Zhongying
Mc Donnell, Rory J.
Denver, Dee R.
Phylogenetic evidence for the invasion of a commercialized European Phasmarhabditis hermaphrodita lineage into North America and New Zealand
title Phylogenetic evidence for the invasion of a commercialized European Phasmarhabditis hermaphrodita lineage into North America and New Zealand
title_full Phylogenetic evidence for the invasion of a commercialized European Phasmarhabditis hermaphrodita lineage into North America and New Zealand
title_fullStr Phylogenetic evidence for the invasion of a commercialized European Phasmarhabditis hermaphrodita lineage into North America and New Zealand
title_full_unstemmed Phylogenetic evidence for the invasion of a commercialized European Phasmarhabditis hermaphrodita lineage into North America and New Zealand
title_short Phylogenetic evidence for the invasion of a commercialized European Phasmarhabditis hermaphrodita lineage into North America and New Zealand
title_sort phylogenetic evidence for the invasion of a commercialized european phasmarhabditis hermaphrodita lineage into north america and new zealand
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430733/
https://www.ncbi.nlm.nih.gov/pubmed/32804938
http://dx.doi.org/10.1371/journal.pone.0237249
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