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Grape seed extract alleviates radiation-induced damages in human blood lymphocytes
OBJECTIVE: Ionizing radiation induces deleterious effects in the biological systems by producing free radicals. Grape Seed Extract (GSE) as a free radical scavenger could protect the body against the damages. MATERIALS AND METHODS: In this study, 12 healthy male volunteers were divided into Groups 1...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Mashhad University of Medical Sciences
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430961/ https://www.ncbi.nlm.nih.gov/pubmed/32850296 |
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author | Ghasemnezhad Targhi, Reza Saba, Valiallah |
author_facet | Ghasemnezhad Targhi, Reza Saba, Valiallah |
author_sort | Ghasemnezhad Targhi, Reza |
collection | PubMed |
description | OBJECTIVE: Ionizing radiation induces deleterious effects in the biological systems by producing free radicals. Grape Seed Extract (GSE) as a free radical scavenger could protect the body against the damages. MATERIALS AND METHODS: In this study, 12 healthy male volunteers were divided into Groups 1, 2, 3 and 4 and received 100, 300, 600 and 1000 mg GSE, respectively. Peripheral blood samples were collected from each volunteer 15 min before, and 1, 2, and 5 hr after GSE oral administration. Blood samples were then irradiated with 150 cGy of 100 kvp X-ray (Irradiated control group, was treated with only 1.5 Gy of X-rays). Cytogenic damages were detected by micronucleus assay. RESULTS: Results showed that irradiation significantly increased the incidence of micronuclei (p<0. 001). In group 1, the mean reduction of micronucleus rate was 26.53%, 34.92%, and 31.38%, 1, 2, and 5 hr after GSE ingestion (p<0.001), respectively; this variable in group 2 was 17.38, 38.33, and 31.38 (p<0. 001), in group 3, was 35.65%, 46%, and 37.15% (p<0.001), respectively and in group 4, was 41.35%, 51.73%, and 50.55% (p<0.0001), respectively. The samples collected 1, 2, and 5 hr after ingestion of GSE exhibited a significant decrease in the incidence of micronuclei compared with the radiation control group. The maximum protection and reduction in frequency of micronuclei (51.73%) was observed 2 hr after ingestion of 1000 mg GSE. CONCLUSION: Consumption of GSE before undergoing radiation protects human lymphocytes against X-rays by reducing radiation-induced genotoxicity. |
format | Online Article Text |
id | pubmed-7430961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-74309612020-08-25 Grape seed extract alleviates radiation-induced damages in human blood lymphocytes Ghasemnezhad Targhi, Reza Saba, Valiallah Avicenna J Phytomed Original Research Article OBJECTIVE: Ionizing radiation induces deleterious effects in the biological systems by producing free radicals. Grape Seed Extract (GSE) as a free radical scavenger could protect the body against the damages. MATERIALS AND METHODS: In this study, 12 healthy male volunteers were divided into Groups 1, 2, 3 and 4 and received 100, 300, 600 and 1000 mg GSE, respectively. Peripheral blood samples were collected from each volunteer 15 min before, and 1, 2, and 5 hr after GSE oral administration. Blood samples were then irradiated with 150 cGy of 100 kvp X-ray (Irradiated control group, was treated with only 1.5 Gy of X-rays). Cytogenic damages were detected by micronucleus assay. RESULTS: Results showed that irradiation significantly increased the incidence of micronuclei (p<0. 001). In group 1, the mean reduction of micronucleus rate was 26.53%, 34.92%, and 31.38%, 1, 2, and 5 hr after GSE ingestion (p<0.001), respectively; this variable in group 2 was 17.38, 38.33, and 31.38 (p<0. 001), in group 3, was 35.65%, 46%, and 37.15% (p<0.001), respectively and in group 4, was 41.35%, 51.73%, and 50.55% (p<0.0001), respectively. The samples collected 1, 2, and 5 hr after ingestion of GSE exhibited a significant decrease in the incidence of micronuclei compared with the radiation control group. The maximum protection and reduction in frequency of micronuclei (51.73%) was observed 2 hr after ingestion of 1000 mg GSE. CONCLUSION: Consumption of GSE before undergoing radiation protects human lymphocytes against X-rays by reducing radiation-induced genotoxicity. Mashhad University of Medical Sciences 2020 /pmc/articles/PMC7430961/ /pubmed/32850296 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Article Ghasemnezhad Targhi, Reza Saba, Valiallah Grape seed extract alleviates radiation-induced damages in human blood lymphocytes |
title | Grape seed extract alleviates radiation-induced damages in human blood lymphocytes |
title_full | Grape seed extract alleviates radiation-induced damages in human blood lymphocytes |
title_fullStr | Grape seed extract alleviates radiation-induced damages in human blood lymphocytes |
title_full_unstemmed | Grape seed extract alleviates radiation-induced damages in human blood lymphocytes |
title_short | Grape seed extract alleviates radiation-induced damages in human blood lymphocytes |
title_sort | grape seed extract alleviates radiation-induced damages in human blood lymphocytes |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430961/ https://www.ncbi.nlm.nih.gov/pubmed/32850296 |
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