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Grape seed extract alleviates radiation-induced damages in human blood lymphocytes

OBJECTIVE: Ionizing radiation induces deleterious effects in the biological systems by producing free radicals. Grape Seed Extract (GSE) as a free radical scavenger could protect the body against the damages. MATERIALS AND METHODS: In this study, 12 healthy male volunteers were divided into Groups 1...

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Autores principales: Ghasemnezhad Targhi, Reza, Saba, Valiallah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430961/
https://www.ncbi.nlm.nih.gov/pubmed/32850296
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author Ghasemnezhad Targhi, Reza
Saba, Valiallah
author_facet Ghasemnezhad Targhi, Reza
Saba, Valiallah
author_sort Ghasemnezhad Targhi, Reza
collection PubMed
description OBJECTIVE: Ionizing radiation induces deleterious effects in the biological systems by producing free radicals. Grape Seed Extract (GSE) as a free radical scavenger could protect the body against the damages. MATERIALS AND METHODS: In this study, 12 healthy male volunteers were divided into Groups 1, 2, 3 and 4 and received 100, 300, 600 and 1000 mg GSE, respectively. Peripheral blood samples were collected from each volunteer 15 min before, and 1, 2, and 5 hr after GSE oral administration. Blood samples were then irradiated with 150 cGy of 100 kvp X-ray (Irradiated control group, was treated with only 1.5 Gy of X-rays). Cytogenic damages were detected by micronucleus assay. RESULTS: Results showed that irradiation significantly increased the incidence of micronuclei (p<0. 001). In group 1, the mean reduction of micronucleus rate was 26.53%, 34.92%, and 31.38%, 1, 2, and 5 hr after GSE ingestion (p<0.001), respectively; this variable in group 2 was 17.38, 38.33, and 31.38 (p<0. 001), in group 3, was 35.65%, 46%, and 37.15% (p<0.001), respectively and in group 4, was 41.35%, 51.73%, and 50.55% (p<0.0001), respectively. The samples collected 1, 2, and 5 hr after ingestion of GSE exhibited a significant decrease in the incidence of micronuclei compared with the radiation control group. The maximum protection and reduction in frequency of micronuclei (51.73%) was observed 2 hr after ingestion of 1000 mg GSE. CONCLUSION: Consumption of GSE before undergoing radiation protects human lymphocytes against X-rays by reducing radiation-induced genotoxicity.
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spelling pubmed-74309612020-08-25 Grape seed extract alleviates radiation-induced damages in human blood lymphocytes Ghasemnezhad Targhi, Reza Saba, Valiallah Avicenna J Phytomed Original Research Article OBJECTIVE: Ionizing radiation induces deleterious effects in the biological systems by producing free radicals. Grape Seed Extract (GSE) as a free radical scavenger could protect the body against the damages. MATERIALS AND METHODS: In this study, 12 healthy male volunteers were divided into Groups 1, 2, 3 and 4 and received 100, 300, 600 and 1000 mg GSE, respectively. Peripheral blood samples were collected from each volunteer 15 min before, and 1, 2, and 5 hr after GSE oral administration. Blood samples were then irradiated with 150 cGy of 100 kvp X-ray (Irradiated control group, was treated with only 1.5 Gy of X-rays). Cytogenic damages were detected by micronucleus assay. RESULTS: Results showed that irradiation significantly increased the incidence of micronuclei (p<0. 001). In group 1, the mean reduction of micronucleus rate was 26.53%, 34.92%, and 31.38%, 1, 2, and 5 hr after GSE ingestion (p<0.001), respectively; this variable in group 2 was 17.38, 38.33, and 31.38 (p<0. 001), in group 3, was 35.65%, 46%, and 37.15% (p<0.001), respectively and in group 4, was 41.35%, 51.73%, and 50.55% (p<0.0001), respectively. The samples collected 1, 2, and 5 hr after ingestion of GSE exhibited a significant decrease in the incidence of micronuclei compared with the radiation control group. The maximum protection and reduction in frequency of micronuclei (51.73%) was observed 2 hr after ingestion of 1000 mg GSE. CONCLUSION: Consumption of GSE before undergoing radiation protects human lymphocytes against X-rays by reducing radiation-induced genotoxicity. Mashhad University of Medical Sciences 2020 /pmc/articles/PMC7430961/ /pubmed/32850296 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Article
Ghasemnezhad Targhi, Reza
Saba, Valiallah
Grape seed extract alleviates radiation-induced damages in human blood lymphocytes
title Grape seed extract alleviates radiation-induced damages in human blood lymphocytes
title_full Grape seed extract alleviates radiation-induced damages in human blood lymphocytes
title_fullStr Grape seed extract alleviates radiation-induced damages in human blood lymphocytes
title_full_unstemmed Grape seed extract alleviates radiation-induced damages in human blood lymphocytes
title_short Grape seed extract alleviates radiation-induced damages in human blood lymphocytes
title_sort grape seed extract alleviates radiation-induced damages in human blood lymphocytes
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430961/
https://www.ncbi.nlm.nih.gov/pubmed/32850296
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