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Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time
Recent outbreaks of viral hemorrhagic fevers (VHFs), including Ebola virus disease (EVD) and Lassa fever (LF), highlight the urgent need for sensitive, deployable tests to diagnose these devastating human diseases. Here we develop CRISPR-Cas13a-based (SHERLOCK) diagnostics targeting Ebola virus (EBO...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7431545/ https://www.ncbi.nlm.nih.gov/pubmed/32807807 http://dx.doi.org/10.1038/s41467-020-17994-9 |
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| author | Barnes, Kayla G. Lachenauer, Anna E. Nitido, Adam Siddiqui, Sameed Gross, Robin Beitzel, Brett Siddle, Katherine J. Freije, Catherine A. Dighero-Kemp, Bonnie Mehta, Samar B. Carter, Amber Uwanibe, Jessica Ajogbasile, Fehintola Olumade, Testimony Odia, Ikponmwosa Sandi, John Demby Momoh, Mambu Metsky, Hayden C. Boehm, Chloe K. Lin, Aaron E. Kemball, Molly Park, Daniel J. Branco, Luis Boisen, Matt Sullivan, Brian Amare, Mihret F. Tiamiyu, Abdulwasiu B. Parker, Zahra F. Iroezindu, Michael Grant, Donald S. Modjarrad, Kayvon Myhrvold, Cameron Garry, Robert F. Palacios, Gustavo Hensley, Lisa E. Schaffner, Stephen F. Happi, Christian T. Colubri, Andres Sabeti, Pardis C. |
| author_facet | Barnes, Kayla G. Lachenauer, Anna E. Nitido, Adam Siddiqui, Sameed Gross, Robin Beitzel, Brett Siddle, Katherine J. Freije, Catherine A. Dighero-Kemp, Bonnie Mehta, Samar B. Carter, Amber Uwanibe, Jessica Ajogbasile, Fehintola Olumade, Testimony Odia, Ikponmwosa Sandi, John Demby Momoh, Mambu Metsky, Hayden C. Boehm, Chloe K. Lin, Aaron E. Kemball, Molly Park, Daniel J. Branco, Luis Boisen, Matt Sullivan, Brian Amare, Mihret F. Tiamiyu, Abdulwasiu B. Parker, Zahra F. Iroezindu, Michael Grant, Donald S. Modjarrad, Kayvon Myhrvold, Cameron Garry, Robert F. Palacios, Gustavo Hensley, Lisa E. Schaffner, Stephen F. Happi, Christian T. Colubri, Andres Sabeti, Pardis C. |
| author_sort | Barnes, Kayla G. |
| collection | PubMed |
| description | Recent outbreaks of viral hemorrhagic fevers (VHFs), including Ebola virus disease (EVD) and Lassa fever (LF), highlight the urgent need for sensitive, deployable tests to diagnose these devastating human diseases. Here we develop CRISPR-Cas13a-based (SHERLOCK) diagnostics targeting Ebola virus (EBOV) and Lassa virus (LASV), with both fluorescent and lateral flow readouts. We demonstrate on laboratory and clinical samples the sensitivity of these assays and the capacity of the SHERLOCK platform to handle virus-specific diagnostic challenges. We perform safety testing to demonstrate the efficacy of our HUDSON protocol in heat-inactivating VHF viruses before SHERLOCK testing, eliminating the need for an extraction. We develop a user-friendly protocol and mobile application (HandLens) to report results, facilitating SHERLOCK’s use in endemic regions. Finally, we successfully deploy our tests in Sierra Leone and Nigeria in response to recent outbreaks. |
| format | Online Article Text |
| id | pubmed-7431545 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-74315452020-08-28 Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time Barnes, Kayla G. Lachenauer, Anna E. Nitido, Adam Siddiqui, Sameed Gross, Robin Beitzel, Brett Siddle, Katherine J. Freije, Catherine A. Dighero-Kemp, Bonnie Mehta, Samar B. Carter, Amber Uwanibe, Jessica Ajogbasile, Fehintola Olumade, Testimony Odia, Ikponmwosa Sandi, John Demby Momoh, Mambu Metsky, Hayden C. Boehm, Chloe K. Lin, Aaron E. Kemball, Molly Park, Daniel J. Branco, Luis Boisen, Matt Sullivan, Brian Amare, Mihret F. Tiamiyu, Abdulwasiu B. Parker, Zahra F. Iroezindu, Michael Grant, Donald S. Modjarrad, Kayvon Myhrvold, Cameron Garry, Robert F. Palacios, Gustavo Hensley, Lisa E. Schaffner, Stephen F. Happi, Christian T. Colubri, Andres Sabeti, Pardis C. Nat Commun Article Recent outbreaks of viral hemorrhagic fevers (VHFs), including Ebola virus disease (EVD) and Lassa fever (LF), highlight the urgent need for sensitive, deployable tests to diagnose these devastating human diseases. Here we develop CRISPR-Cas13a-based (SHERLOCK) diagnostics targeting Ebola virus (EBOV) and Lassa virus (LASV), with both fluorescent and lateral flow readouts. We demonstrate on laboratory and clinical samples the sensitivity of these assays and the capacity of the SHERLOCK platform to handle virus-specific diagnostic challenges. We perform safety testing to demonstrate the efficacy of our HUDSON protocol in heat-inactivating VHF viruses before SHERLOCK testing, eliminating the need for an extraction. We develop a user-friendly protocol and mobile application (HandLens) to report results, facilitating SHERLOCK’s use in endemic regions. Finally, we successfully deploy our tests in Sierra Leone and Nigeria in response to recent outbreaks. Nature Publishing Group UK 2020-08-17 /pmc/articles/PMC7431545/ /pubmed/32807807 http://dx.doi.org/10.1038/s41467-020-17994-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| spellingShingle | Article Barnes, Kayla G. Lachenauer, Anna E. Nitido, Adam Siddiqui, Sameed Gross, Robin Beitzel, Brett Siddle, Katherine J. Freije, Catherine A. Dighero-Kemp, Bonnie Mehta, Samar B. Carter, Amber Uwanibe, Jessica Ajogbasile, Fehintola Olumade, Testimony Odia, Ikponmwosa Sandi, John Demby Momoh, Mambu Metsky, Hayden C. Boehm, Chloe K. Lin, Aaron E. Kemball, Molly Park, Daniel J. Branco, Luis Boisen, Matt Sullivan, Brian Amare, Mihret F. Tiamiyu, Abdulwasiu B. Parker, Zahra F. Iroezindu, Michael Grant, Donald S. Modjarrad, Kayvon Myhrvold, Cameron Garry, Robert F. Palacios, Gustavo Hensley, Lisa E. Schaffner, Stephen F. Happi, Christian T. Colubri, Andres Sabeti, Pardis C. Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time |
| title | Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time |
| title_full | Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time |
| title_fullStr | Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time |
| title_full_unstemmed | Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time |
| title_short | Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time |
| title_sort | deployable crispr-cas13a diagnostic tools to detect and report ebola and lassa virus cases in real-time |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7431545/ https://www.ncbi.nlm.nih.gov/pubmed/32807807 http://dx.doi.org/10.1038/s41467-020-17994-9 |
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