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Adoptive transfer of bone marrow-derived dendritic cells (BMDCs) alleviates OVA-induced allergic airway inflammation in asthmatic mice

Airway dendritic cells (DCs) are recognized as important factors in the mechanisms of allergic inflammatory diseases. Suppressor of cytokine signaling 3 (SOCS3) is involved in regulating the functions of T cells and macrophages, but the roles of SOCS3-expressing DCs in the pathogeneses of allergic i...

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Autores principales: Xu, Kan, Wu, Nan, Min, Zhihui, Li, Zheng, Zhu, Tao, Liu, Chunfang, Zeng, Yuzhen, Song, Juan, Mao, Ruolin, Ji, Hong, Jiang, Zhilong, Chen, Zhihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7431555/
https://www.ncbi.nlm.nih.gov/pubmed/32807859
http://dx.doi.org/10.1038/s41598-020-70467-3
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author Xu, Kan
Wu, Nan
Min, Zhihui
Li, Zheng
Zhu, Tao
Liu, Chunfang
Zeng, Yuzhen
Song, Juan
Mao, Ruolin
Ji, Hong
Jiang, Zhilong
Chen, Zhihong
author_facet Xu, Kan
Wu, Nan
Min, Zhihui
Li, Zheng
Zhu, Tao
Liu, Chunfang
Zeng, Yuzhen
Song, Juan
Mao, Ruolin
Ji, Hong
Jiang, Zhilong
Chen, Zhihong
author_sort Xu, Kan
collection PubMed
description Airway dendritic cells (DCs) are recognized as important factors in the mechanisms of allergic inflammatory diseases. Suppressor of cytokine signaling 3 (SOCS3) is involved in regulating the functions of T cells and macrophages, but the roles of SOCS3-expressing DCs in the pathogeneses of allergic inflammatory diseases are still controversial. We compared the effects of adoptively transferred SOCS3(−/−) and SOCS3(+/+) bone marrow-derived DCs (BMDCs) on airway inflammation in ovalbumin (OVA)-sensitized asthmatic mice. Adoptive transfer of mature DCs (lipopolysaccharide [LPS]-induced DCs, DClps) with or without SOCS3 gene expression significantly ameliorated allergic airway inflammation. SOCS3(−/−) DCs slightly attenuated BMDC-induced immunogenic tolerance. DClps migrated to OVA-sensitized lungs with higher efficiency than immature DCs (DCim). DClps with or without SOCS3 greatly improved lung pathology scores and alleviated airway inflammatory cell infiltration after adoptive transfer into mice; they also increased interleukin-10 (IL-10) and transforming growth factor-β (TGF-β) production and inhibited signal transducer and activator of transcription (STAT) 4 and STAT6 signaling in the lungs after OVA sensitization. In conclusion, the BMDC adoptive transfer-induced immunogenic tolerance in OVA-sensitized mice might not be due to SOCS3 gene depletion. BMDC adoptive transfer may be developed into a new approach that alleviates asthma by modulating the balance between immune tolerance and inflammation.
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spelling pubmed-74315552020-08-18 Adoptive transfer of bone marrow-derived dendritic cells (BMDCs) alleviates OVA-induced allergic airway inflammation in asthmatic mice Xu, Kan Wu, Nan Min, Zhihui Li, Zheng Zhu, Tao Liu, Chunfang Zeng, Yuzhen Song, Juan Mao, Ruolin Ji, Hong Jiang, Zhilong Chen, Zhihong Sci Rep Article Airway dendritic cells (DCs) are recognized as important factors in the mechanisms of allergic inflammatory diseases. Suppressor of cytokine signaling 3 (SOCS3) is involved in regulating the functions of T cells and macrophages, but the roles of SOCS3-expressing DCs in the pathogeneses of allergic inflammatory diseases are still controversial. We compared the effects of adoptively transferred SOCS3(−/−) and SOCS3(+/+) bone marrow-derived DCs (BMDCs) on airway inflammation in ovalbumin (OVA)-sensitized asthmatic mice. Adoptive transfer of mature DCs (lipopolysaccharide [LPS]-induced DCs, DClps) with or without SOCS3 gene expression significantly ameliorated allergic airway inflammation. SOCS3(−/−) DCs slightly attenuated BMDC-induced immunogenic tolerance. DClps migrated to OVA-sensitized lungs with higher efficiency than immature DCs (DCim). DClps with or without SOCS3 greatly improved lung pathology scores and alleviated airway inflammatory cell infiltration after adoptive transfer into mice; they also increased interleukin-10 (IL-10) and transforming growth factor-β (TGF-β) production and inhibited signal transducer and activator of transcription (STAT) 4 and STAT6 signaling in the lungs after OVA sensitization. In conclusion, the BMDC adoptive transfer-induced immunogenic tolerance in OVA-sensitized mice might not be due to SOCS3 gene depletion. BMDC adoptive transfer may be developed into a new approach that alleviates asthma by modulating the balance between immune tolerance and inflammation. Nature Publishing Group UK 2020-08-17 /pmc/articles/PMC7431555/ /pubmed/32807859 http://dx.doi.org/10.1038/s41598-020-70467-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Xu, Kan
Wu, Nan
Min, Zhihui
Li, Zheng
Zhu, Tao
Liu, Chunfang
Zeng, Yuzhen
Song, Juan
Mao, Ruolin
Ji, Hong
Jiang, Zhilong
Chen, Zhihong
Adoptive transfer of bone marrow-derived dendritic cells (BMDCs) alleviates OVA-induced allergic airway inflammation in asthmatic mice
title Adoptive transfer of bone marrow-derived dendritic cells (BMDCs) alleviates OVA-induced allergic airway inflammation in asthmatic mice
title_full Adoptive transfer of bone marrow-derived dendritic cells (BMDCs) alleviates OVA-induced allergic airway inflammation in asthmatic mice
title_fullStr Adoptive transfer of bone marrow-derived dendritic cells (BMDCs) alleviates OVA-induced allergic airway inflammation in asthmatic mice
title_full_unstemmed Adoptive transfer of bone marrow-derived dendritic cells (BMDCs) alleviates OVA-induced allergic airway inflammation in asthmatic mice
title_short Adoptive transfer of bone marrow-derived dendritic cells (BMDCs) alleviates OVA-induced allergic airway inflammation in asthmatic mice
title_sort adoptive transfer of bone marrow-derived dendritic cells (bmdcs) alleviates ova-induced allergic airway inflammation in asthmatic mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7431555/
https://www.ncbi.nlm.nih.gov/pubmed/32807859
http://dx.doi.org/10.1038/s41598-020-70467-3
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